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Cutting Edge |
Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110
We report in this work the expression and characterization of
class I molecules expressed as single-chain trimers consisting of an
antigenic peptide-spacer-
2-microglobulin-spacer H chain.
Our results indicate that these single-chain constructs assemble
efficiently, maintain their covalent structure, and are unusually
stable at the cell surface. Consequently, these constructs are at least
1000-fold less accessible to exogenous peptide than class I molecules
loaded with endogenous peptides, and they are potent simulators of
peptide-specific CTL and Abs. Our combined findings suggest that
single-chain trimers may have applications as DNA vaccines against
virus infection or tumors.
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