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*
Departments of Medicine II and Gastroenterology,
Microbiology, and
Pathology, Technical University of Munich, Munich, Germany
Presence of the Helicobacter pylori adherence factor
blood group Ag-binding adhesin (BabA; binding to Lewisb
(Leb)) is associated with ulcer disease, adenocarcinoma,
and precancerous lesions. The importance of BabA for bacterial
colonization and the inflammatory response is unknown. A total of 141
antral biopsies from H. pylori-infected patients were
assessed in regard to the degree of granulocytic (G0°–G3°) and
lymphocytic (L1°–L3°) infiltration. DNA genotypes of
babA2 (the transcriptionally active gene of BabA),
cagA, and vacAs1/2 were determined by
PCR. Colonization density and Leb status on gastric
epithelial cells were determined by immunohistochemistry. Real-time
quantitative (TaqMan) RT-PCR determined mRNA expression of IL-8, TNF
-
, and the Th1 markers IFN-
and the IL-12R 
2 chain. A total of
91% of infected patients were Leb positive. The
vacAs1+/cagA+
strains harboring babA2 showed significantly higher
levels of granulocytic infiltration, bacterial colonization, and IL-8
mRNA than
vacAs1+/cagA+
strains lacking babA2. IL-8 mRNA and protein production
by KATO III cells in vitro increased dose dependently with
addition of different numbers of type 1 strains (G27 and
2808 strains, 0.1–20 bacteria/cell). The mRNA expression of TNF-,
IFN-, and IL-12R 2 was higher in H.
pylori-positive patients than in controls, but it did not
differ significantly between patients infected with different strain
types. These data suggest that BabA facilitates colonization of
H. pylori and thereby increases IL-8 response, resulting
in enhanced mucosal inflammation. Infection with strains harboring BabA
thereby augment a nonspecific immune response, whereas the Th1 response
toward H. pylori appears to be independent of BabA,
cytotoxin-associated gene A, or vacuolating
cytotoxin.
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