| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, and
Division of Hematology, Clinical Immunology, Department of Clinical and Experimental Medicine, University of Perugia, Perugia, Italy;
Microbiology Section, Department of Experimental Medicine, University of Rome, Tor Vergata, Rome, Italy; and
Department of Microbiology, Montana State University, Bozeman, MT 59717
Immature myeloid dendritic cells (DC) phagocytose yeasts and hyphae
of the fungus Candida albicans and induce different Th
cell responses to the fungus. Ingestion of yeasts activates DC for
production of IL-12 and Th1 priming, while ingestion of hyphae induces
IL-4 production and Th2 priming. In vivo, generation of antifungal
protective immunity is induced upon injection of DC ex vivo pulsed with
Candida yeasts but not hyphae. In the present study we
sought to determine the functional activity of DC transfected with
yeast or hyphal RNA. It was found that DC, from either spleens or bone
marrow, transfected with yeast, but not hyphal, RNA 1) express fungal
mannoproteins on their surface; 2) undergo functional maturation, as
revealed by the up-regulated expression of MHC class II Ags and
costimulatory molecules; 3) produce IL-12 but no IL-4; 4) are capable
of inducing Th1-dependent antifungal resistance when delivered s.c. in
vivo in nontransplanted mice; and 5) provide protection against the
fungus in allogeneic bone marrow-transplanted mice, by accelerating the
functional recovery of Candida-specific
IFN-
-producing CD4+ donor lymphocytes. These results
indicate the efficacy of DC pulsed with Candida yeasts
or yeast RNA as fungal vaccines and point to the potential use of
RNA-transfected DC as anti-infective vaccines in conditions that
negate the use of attenuated microorganisms or in the case of poor
availability of protective Ags.
This article has been cited by other articles:
|
|
 |
|
  P. Lupo, Y. C. Chang, B. L. Kelsall, J. M. Farber, D. Pietrella, A. Vecchiarelli, F. Leon, and K. J. Kwon-Chung The Presence of Capsule in Cryptococcus neoformans Influences the Gene Expression Profile in Dendritic Cells during Interaction with the Fungus Infect. Immun., April 1, 2008; 76(4): 1581 - 1589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. De Luca, C. Montagnoli, T. Zelante, P. Bonifazi, S. Bozza, S. Moretti, C. D'Angelo, C. Vacca, L. Boon, F. Bistoni, et al. Functional yet Balanced Reactivity to Candida albicans Requires TRIF, MyD88, and IDO-Dependent Inhibition of Rorc J. Immunol., November 1, 2007; 179(9): 5999 - 6008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Wang, L. Xiang, J. Shao, and Z. Yuan The 3' CCACCA Sequence of tRNAAla(UGC) Is the Motif That Is Important in Inducing Th1-Like Immune Response, and This Motif Can Be Recognized by Toll-Like Receptor 3. Clin. Vaccine Immunol., July 1, 2006; 13(7): 733 - 739. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. De Bernardis, R. Lucciarini, M. Boccanera, C. Amantini, S. Arancia, S. Morrone, M. Mosca, A. Cassone, and G. Santoni Phenotypic and Functional Characterization of Vaginal Dendritic Cells in a Rat Model of Candida albicans Vaginitis Infect. Immun., July 1, 2006; 74(7): 4282 - 4294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. LeBlanc, M. M. Barousse, and P. L. Fidel Jr. Role for Dendritic Cells in Immunoregulation during Experimental Vaginal Candidiasis. Infect. Immun., June 1, 2006; 74(6): 3213 - 3221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Awasthi, V. Awasthi, D. M. Magee, and J. J. Coalson Efficacy of Antigen 2/Proline-Rich Antigen cDNA-Transfected Dendritic Cells in Immunization of Mice against Coccidioides posadasii J. Immunol., September 15, 2005; 175(6): 3900 - 3906. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. de Repentigny, D. Lewandowski, and P. Jolicoeur Immunopathogenesis of Oropharyngeal Candidiasis in Human Immunodeficiency Virus Infection Clin. Microbiol. Rev., October 1, 2004; 17(4): 729 - 759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bellocchio, C. Montagnoli, S. Bozza, R. Gaziano, G. Rossi, S. S. Mambula, A. Vecchi, A. Mantovani, S. M. Levitz, and L. Romani The Contribution of the Toll-Like/IL-1 Receptor Superfamily to Innate and Adaptive Immunity to Fungal Pathogens In Vivo J. Immunol., March 1, 2004; 172(5): 3059 - 3069. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Romani, C. Montagnoli, S. Bozza, K. Perruccio, A. Spreca, P. Allavena, S. Verbeek, R. A. Calderone, F. Bistoni, and P. Puccetti The exploitation of distinct recognition receptors in dendritic cells determines the full range of host immune relationships with Candida albicans Int. Immunol., January 1, 2004; 16(1): 149 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bozza, K. Perruccio, C. Montagnoli, R. Gaziano, S. Bellocchio, E. Burchielli, G. Nkwanyuo, L. Pitzurra, A. Velardi, and L. Romani A dendritic cell vaccine against invasive aspergillosis in allogeneic hematopoietic transplantation Blood, November 15, 2003; 102(10): 3807 - 3814. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Montagnoli, A. Bacci, S. Bozza, R. Gaziano, P. Mosci, A. H. Sharpe, and L. Romani B7/CD28-Dependent CD4+CD25+ Regulatory T Cells Are Essential Components of the Memory-Protective Immunity to Candida albicans J. Immunol., December 1, 2002; 169(11): 6298 - 6308. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
