| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago, IL 60612; and
Department of Pathology, Program in Immunology, Tufts University School of Medicine, Boston, MA 02111
Ig class switch recombination (CSR) occurs by an intrachromosomal
deletional process between switch (S) regions in B cells. To facilitate
the study of CSR, we derived a new B cell line, 1.B4.B6, which is
uniquely capable of µ
3, µ
, and µ
, but not
µ1 CSR at its endogenous loci. The 1.B4.B6 cell line was used
in combination with plasmid-based isotype-specific S substrates in
transient transfection assays to test for the presence of trans-acting
switching activities. The 1.B4.B6 cell line supports µ3, but
not µ1 recombination, on S substrates. In contrast, normal
splenic B cells activated with LPS and IL-4 are capable of
plasmid-based µ1 CSR and demonstrate that this S plasmid is
active. Activation-induced deaminase (AID) was used as a marker to
identify existing B cell lines as possible candidates for supporting
CSR. The M12 and A20 cell lines were identified as AID positive and,
following activation with CD40L and other activators, were found to
differentially support µ and µ plasmid-based CSR. These
studies provide evidence for two new switching activities for
µ1 and µ CSR, which are distinct from µ3 and
µ switching activities previously described. AID is expressed
in all the B cell lines capable of CSR, but cannot account for the
isotype specificity defined by the S plasmid assay. These results are
consistent with a model in which isotype-specific switching factors are
either isotype-specific recombinases or DNA binding proteins with
sequence specificity for S DNA.
This article has been cited by other articles:
|
|
 |
|
  L. Wang, R. Wuerffel, S. Feldman, A. A. Khamlichi, and A. L. Kenter S region sequence, RNA polymerase II, and histone modifications create chromatin accessibility during class switch recombination J. Exp. Med., August 3, 2009; 206(8): 1817 - 1830. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Zarrin, P. H. Goff, K. Senger, and F. W. Alt S{gamma}3 switch sequences function in place of endogenous S{gamma}1 to mediate antibody class switching J. Exp. Med., July 7, 2008; 205(7): 1567 - 1572. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. P. O'Connor, L. A. Vogel, W. Zhang, W. Loo, D. Shnider, E. F. Lind, M. Ratliff, R. J. Noelle, and L. D. Erickson Imprinting the Fate of Antigen-Reactive B Cells through the Affinity of the B Cell Receptor J. Immunol., December 1, 2006; 177(11): 7723 - 7732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Bradley, D. A. Kaminski, A. H. F. M. Peters, T. Jenuwein, and J. Stavnezer The Histone Methyltransferase Suv39h1 Increases Class Switch Recombination Specifically to IgA J. Immunol., July 15, 2006; 177(2): 1179 - 1188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wang, N. Whang, R. Wuerffel, and A. L. Kenter AID-dependent histone acetylation is detected in immunoglobulin S regions J. Exp. Med., January 23, 2006; 203(1): 215 - 226. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Li, Z. Luo, and M. D. Scharff Differential regulation of histone acetylation and generation of mutations in switch regions is associated with Ig class switching PNAS, October 26, 2004; 101(43): 15428 - 15433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. X. Spillmann and M. Wabl Endogenous Expression of Activation-Induced Cytidine Deaminase in Cell Line WEHI-231 J. Immunol., August 1, 2004; 173(3): 1858 - 1867. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Wang and M. Wabl DNA Acrobats of the Ig Class Switch J. Immunol., May 15, 2004; 172(10): 5815 - 5821. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Khamlichi, F. Glaudet, Z. Oruc, V. Denis, M. Le Bert, and M. Cogne Immunoglobulin class-switch recombination in mice devoid of any S{micro} tandem repeat Blood, May 15, 2004; 103(10): 3828 - 3836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Kenter, R. Wuerffel, C. Dominguez, A. Shanmugam, and H. Zhang Mapping of a Functional Recombination Motif that Defines Isotype Specificity for {micro}->{gamma}3 Switch Recombination Implicates NF-{kappa}B p50 as the Isotype-specific Switching Factor J. Exp. Med., March 1, 2004; 199(5): 617 - 627. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Schrader, J. Vardo, and J. Stavnezer Mlh1 Can Function in Antibody Class Switch Recombination Independently of Msh2 J. Exp. Med., May 19, 2003; 197(10): 1377 - 1383. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Zhang Accessibility control and machinery of immunoglobulin class switch recombination J. Leukoc. Biol., March 1, 2003; 73(3): 323 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E. Drouin, C. E. Schrader, J. Stavnezer, and U. Hansen The Ubiquitously Expressed DNA-Binding Protein Late SV40 Factor Binds Ig Switch Regions and Represses Class Switching to IgA J. Immunol., March 15, 2002; 168(6): 2847 - 2856. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
