| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
,
*
Department of Immunology, University of Washington, Seattle, WA 98185;
Institute for Systems Biology, Seattle, WA 98105;
Department of Biotechnology, University of Washington, Seattle, WA 98195;
Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226;
¶ Department of Obstetrics and Gynaecology, St. Mary’s Hospital, University of Manchester, Manchester, United Kingdom; and
||
Department of Medicine, Monash University, Melbourne, Australia
After i.p. infection of mice with the intracellular bacterium
Mycobacterium bovis bacillus Calmette-Guérin,
macrophages recovered from the peritoneal cavity display classical
signs of immune activation. We have identified a member of the serine
protease inhibitor (serpin) family which is highly induced in
macrophages during bacillus Calmette-Guérin infection. Serpin 2a
(spi2a) expression is also induced in macrophages in vivo during
infection with Salmonella typhimurium and
Listeria monocytogenes, and in vitro by a variety of
bacteria and bacterial products. The cytokine IFN-
also induces
spi2a expression in macrophages, and this induction is synergistic with
bacterial products. We also demonstrate here that a ubiquitin homolog,
IFN-stimulated gene of 15-kDa (ISG15), is strongly induced during in
vitro and in vivo activation of macrophages and that it conjugates to
spi2a in activated macrophages. The ISG15-spi2a conjugates were
identified by tandem mass spectrometry and contained spi2a conjugated
to either one or two molecules of ISG15. Whereas spi2a was induced by
either bacterial products or IFN-, ISG15 was induced only by
bacterial products. Although many protein targets have been described
for ubiquitin conjugation, spi2a is the first ISG15-modified protein to
be reported. Macrophage activation is accompanied by the activation of
a variety of proteases. It is of interest that a member of the serine
protease inhibitor family is concomitantly induced and modified by a
ubiquitin-like protein.
This article has been cited by other articles:
|
|
 |
|
  C. Lai, J. J. Struckhoff, J. Schneider, L. Martinez-Sobrido, T. Wolff, A. Garcia-Sastre, D.-E. Zhang, and D. J. Lenschow Mice Lacking the ISG15 E1 Enzyme UbE1L Demonstrate Increased Susceptibility to both Mouse-Adapted and Non-Mouse-Adapted Influenza B Virus Infection J. Virol., January 15, 2009; 83(2): 1147 - 1151. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Herrmann, L. O. Lerman, and A. Lerman Ubiquitin and Ubiquitin-Like Proteins in Protein Regulation Circ. Res., May 11, 2007; 100(9): 1276 - 1291. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Andersson, B. Hartmanova, R. KuoLee, P. Ryden, W. Conlan, W. Chen, and A. Sjostedt Transcriptional profiling of host responses in mouse lungs following aerosol infection with type A Francisella tularensis. J. Med. Microbiol., March 1, 2006; 55(Pt 3): 263 - 271. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Zou and D.-E. Zhang The Interferon-inducible Ubiquitin-protein Isopeptide Ligase (E3) EFP Also Functions as an ISG15 E3 Ligase J. Biol. Chem., February 17, 2006; 281(7): 3989 - 3994. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Klein, S. Bauersachs, S. E. Ulbrich, R. Einspanier, H. H.D. Meyer, S. E.M. Schmidt, H.-D. Reichenbach, M. Vermehren, F. Sinowatz, H. Blum, et al. Monozygotic Twin Model Reveals Novel Embryo-Induced Transcriptome Changes of Bovine Endometrium in the Preattachment Period Biol Reprod, February 1, 2006; 74(2): 253 - 264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. I. Kim, M. Yan, O. Malakhova, J.-K. Luo, M.-F. Shen, W. Zou, J. C. de la Torre, and D.-E. Zhang Ube1L and Protein ISGylation Are Not Essential for Alpha/Beta Interferon Signaling Mol. Cell. Biol., January 15, 2006; 26(2): 472 - 479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Desai, A. L. Haas, L. M. Wood, Y.-C. Tsai, S. Pestka, E. H. Rubin, A. Saleem, A. Nur-E-Kamal, and L. F. Liu Elevated Expression of ISG15 in Tumor Cells Interferes with the Ubiquitin/26S Proteasome Pathway Cancer Res., January 15, 2006; 66(2): 921 - 928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-P. Knobeloch, O. Utermohlen, A. Kisser, M. Prinz, and I. Horak Reexamination of the Role of Ubiquitin-Like Modifier ISG15 in the Phenotype of UBP43-Deficient Mice Mol. Cell. Biol., December 15, 2005; 25(24): 11030 - 11034. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Takeuchi, S. Iwahara, Y. Saeki, H. Sasajima, and H. Yokosawa Link between the Ubiquitin Conjugation System and the ISG15 Conjugation System: ISG15 Conjugation to the UbcH6 Ubiquitin E2 Enzyme J. Biochem., December 1, 2005; 138(6): 711 - 719. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Lenschow, N. V. Giannakopoulos, L. J. Gunn, C. Johnston, A. K. O'Guin, R. E. Schmidt, B. Levine, and H. W. Virgin IV Identification of Interferon-Stimulated Gene 15 as an Antiviral Molecule during Sindbis Virus Infection In Vivo J. Virol., November 15, 2005; 79(22): 13974 - 13983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Osiak, O. Utermohlen, S. Niendorf, I. Horak, and K.-P. Knobeloch ISG15, an Interferon-Stimulated Ubiquitin-Like Protein, Is Not Essential for STAT1 Signaling and Responses against Vesicular Stomatitis and Lymphocytic Choriomeningitis Virus Mol. Cell. Biol., August 1, 2005; 25(15): 6338 - 6345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Narasimhan, M. Wang, Z. Fu, J. M. Klein, A. L. Haas, and J.-J. P. Kim Crystal Structure of the Interferon-induced Ubiquitin-like Protein ISG15 J. Biol. Chem., July 22, 2005; 280(29): 27356 - 27365. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Zhao, C. Denison, J. M. Huibregtse, S. Gygi, and R. M. Krug Human ISG15 conjugation targets both IFN-induced and constitutively expressed proteins functioning in diverse cellular pathways PNAS, July 19, 2005; 102(29): 10200 - 10205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. I. Kim, O. A. Malakhova, K. Hoebe, M. Yan, B. Beutler, and D.-E. Zhang Enhanced Antibacterial Potential in UBP43-Deficient Mice against Salmonella typhimurium Infection by Up-Regulating Type I IFN Signaling J. Immunol., July 15, 2005; 175(2): 847 - 854. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Joyce, F. J. White, R. C. Burghardt, J. J. Muniz, T. E. Spencer, F. W. Bazer, and G. A. Johnson Interferon Stimulated Gene 15 Conjugates to Endometrial Cytosolic Proteins and Is Expressed at the Uterine-Placental Interface throughout Pregnancy in Sheep Endocrinology, February 1, 2005; 146(2): 675 - 684. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Rempel, B. R. Francis, K. J. Austin, and T. R. Hansen Isolation and Sequence of an Interferon-{tau}-Inducible, Pregnancy- and Bovine Interferon-Stimulated Gene Product 15 (ISG15)-Specific, Bovine Ubiquitin-Activating E1-Like (UBE1L) Enzyme Biol Reprod, February 1, 2005; 72(2): 365 - 372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. I. Kim, N. V. Giannakopoulos, H. W. Virgin, and D.-E. Zhang Interferon-Inducible Ubiquitin E2, Ubc8, Is a Conjugating Enzyme for Protein ISGylation Mol. Cell. Biol., November 1, 2004; 24(21): 9592 - 9600. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Staub Ubiquitylation and Isgylation: Overlapping Enzymatic Cascades Do the Job Sci. Signal., August 10, 2004; 2004(245): pe43 - pe43. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Zhao, S. L. Beaudenon, M. L. Kelley, M. B. Waddell, W. Yuan, B. A. Schulman, J. M. Huibregtse, and R. M. Krug The UbcH8 ubiquitin E2 enzyme is also the E2 enzyme for ISG15, an IFN-{alpha}/{beta}-induced ubiquitin-like protein PNAS, May 18, 2004; 101(20): 7578 - 7582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Pitha-Rowe, B. A. Hassel, and E. Dmitrovsky Involvement of UBE1L in ISG15 Conjugation during Retinoid-induced Differentiation of Acute Promyelocytic Leukemia J. Biol. Chem., April 30, 2004; 279(18): 18178 - 18187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hemelaar, A. Borodovsky, B. M. Kessler, D. Reverter, J. Cook, N. Kolli, T. Gan-Erdene, K. D. Wilkinson, G. Gill, C. D. Lima, et al. Specific and Covalent Targeting of Conjugating and Deconjugating Enzymes of Ubiquitin-Like Proteins Mol. Cell. Biol., January 1, 2004; 24(1): 84 - 95. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Gan-Erdene, K. Nagamalleswari, L. Yin, K. Wu, Z.-Q. Pan, and K. D. Wilkinson Identification and Characterization of DEN1, a Deneddylase of the ULP Family J. Biol. Chem., August 1, 2003; 278(31): 28892 - 28900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Malakhov, K. I. Kim, O. A. Malakhova, B. S. Jacobs, E. C. Borden, and D.-E. Zhang High-throughput Immunoblotting. UBIQUITIN-LIKE PROTEIN ISG15 MODIFIES KEY REGULATORS OF SIGNAL TRANSDUCTION J. Biol. Chem., May 2, 2003; 278(19): 16608 - 16613. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Liu, X.-L. Li, and B. A. Hassel Proteasomes Modulate Conjugation to the Ubiquitin-like Protein, ISG15 J. Biol. Chem., January 10, 2003; 278(3): 1594 - 1602. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
