HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nair, D. T. Articles by Salunke, D. M. Search for Related Content PubMed PubMed Citation Articles by Nair, D. T. Articles by Salunke, D. M.

Epitope Recognition by Diverse Antibodies Suggests Conformational Convergence in an Antibody Response¹

Deepak T. Nair^*, Kavita Singh^*, Zaved Siddiqui, Bishnu P. Nayak, Kanury V. S. Rao and Dinakar M. Salunke^2,*

^* Structural Biology Unit, National Institute of Immunology, and Immunology Group, International Center of Genetic Engineering and Biotechnology, New Delhi, India

Crystal structures of distinct mAbs that recognize a common epitope of a peptide Ag have been determined and analyzed in the unbound and bound forms. These Abs display dissimilar binding site structures in the absence of the Ag. The dissimilarity is primarily expressed in the conformations of complementarity-determining region H3, which is responsible for defining the epitope specificity. Interestingly, however, the three Abs exhibit similar complementarity-determining region conformations in the Ag binding site while recognizing the common epitope, indicating that different pathways of binding are used for Ag recognition. The epitope also exhibits conformational similarity when bound to each of these Abs, although the peptide Ag was otherwise flexible. The observed conformational convergence in the epitope and the Ag binding site was facilitated by the plasticity in the nature of interactions.

This article has been cited by other articles:

				G. Fenalti, C. S. Hampe, Y. Arafat, R. H.P. Law, J. P. Banga, I. R. Mackay, J. C. Whisstock, A. M. Buckle, and M. J. Rowley COOH-Terminal Clustering of Autoantibody and T-Cell Determinants on the Structure of GAD65 Provide Insights Into the Molecular Basis of Autoreactivity Diabetes, May 1, 2008; 57(5): 1293 - 1301. [Abstract] [Full Text] [PDF]

				S.-W. Chi, D.-H. Kim, S.-H. Lee, I. Chang, and K.-H. Han Pre-structured motifs in the natively unstructured preS1 surface antigen of hepatitis B virus Protein Sci., October 1, 2007; 16(10): 2108 - 2117. [Abstract] [Full Text] [PDF]

				S. M. McLachlan, Y. Nagayama, and B. Rapoport Insight into Graves' Hyperthyroidism from Animal Models Endocr. Rev., October 1, 2005; 26(6): 800 - 832. [Abstract] [Full Text] [PDF]

				M. Goel, L. Krishnan, S. Kaur, K. J. Kaur, and D. M. Salunke Plasticity within the Antigen-Combining Site May Manifest as Molecular Mimicry in the Humoral Immune Response J. Immunol., December 15, 2004; 173(12): 7358 - 7367. [Abstract] [Full Text] [PDF]

				R. Pantophlet, I. A. Wilson, and D. R. Burton Hyperglycosylated Mutants of Human Immunodeficiency Virus (HIV) Type 1 Monomeric gp120 as Novel Antigens for HIV Vaccine Design J. Virol., May 15, 2003; 77(10): 5889 - 5901. [Abstract] [Full Text] [PDF]

				D. T. Nair, K. J. Kaur, K. Singh, P. Mukherjee, D. Rajagopal, A. George, V. Bal, S. Rath, K. V. S. Rao, and D. M. Salunke Mimicry of Native Peptide Antigens by the Corresponding Retro-Inverso Analogs Is Dependent on Their Intrinsic Structure and Interaction Propensities J. Immunol., February 1, 2003; 170(3): 1362 - 1373. [Abstract] [Full Text] [PDF]