The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Takeba, Y.
Right arrow Articles by Suzuki, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Takeba, Y.
Right arrow Articles by Suzuki, N.
The Journal of Immunology, 2002, 168: 2365-2370.
Copyright © 2002 by The American Association of Immunologists

Txk, a Member of Nonreceptor Tyrosine Kinase of Tec Family, Acts as a Th1 Cell-Specific Transcription Factor and Regulates IFN-{gamma} Gene Transcription1

Yuko Takeba2,*, Hiroko Nagafuchi2,{dagger}, Mitsuhiro Takeno*, Jun-ichi Kashiwakura* and Noboru Suzuki3,*

* Departments of Immunology and Medicine, and {dagger} Institute of Medical Science, St. Marianna University School of Medicine, Kawasaki, Kanagawa, Japan

Precise mechanisms responsible for Th1 cell activation and differentiation are not fully elucidated. We have recently reported that Txk, a member of Tec family nonreceptor tyrosine kinase, is expressed on Th1/Th0 cells, and Txk regulates specifically IFN-{gamma} gene expression. In this study, we found that Txk bound to IFN-{gamma} promoter region. Txk transfection increased transcriptional activity of IFN-{gamma} promoter plus luciferase constructs severalfold, including IFN-{gamma} promoter -538, -208, and -53. IFN-{gamma} promoter -39 was refractory to the Txk transfection. The actual site to which Txk bound was the element consisting of -53 and -39 bp from the transcription start site of human IFN-{gamma} gene, a site distinct from several previously characterized binding sites. We found that the entire -53/-39 region was necessary for the binding to and function of Txk, because mutant promoter oligoDNA that contained contiguous five base substitutions dispersed throughout the -53/-39 inhibited the binding, and the mutant promoters did not respond to the Txk transfection. Similar sequences of this element are found within the 5' flanking regions of several Th1 cell-associated protein genes. Thus, Txk is expressed on Th1/Th0 cells with the IFN-{gamma} production and acts as a Th1 cell-specific transcription factor.




This article has been cited by other articles:


Home page
 J Mol Cell BiolHome page
Y. Y. Wan and R. A. Flavell
How Diverse--CD4 Effector T Cells and their Functions
J Mol Cell Biol, October 1, 2009; 1(1): 20 - 36.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Sahu, A. M. Venegas, D. Jankovic, W. Mitzner, J. Gomez-Rodriguez, J. L. Cannons, C. Sommers, P. Love, A. Sher, P. L. Schwartzberg, et al.
Selective Expression Rather than Specific Function of Txk and Itk Regulate Th1 and Th2 Responses
J. Immunol., November 1, 2008; 181(9): 6125 - 6131.
[Abstract] [Full Text] [PDF]

Home page
 BioinformaticsHome page
L. L. Elo, H. Jarvenpaa, M. Oresic, R. Lahesmaa, and T. Aittokallio
Systematic construction of gene coexpression networks with applications to human T helper cell differentiation process
Bioinformatics, August 15, 2007; 23(16): 2096 - 2103.
[Abstract] [Full Text] [PDF]

Home page
 Clin Med ResHome page
N. Suzuki, K. Nara, and T. Suzuki
Skewed Th1 Responses Caused by Excessive Expression of Txk, a Member of the Tec Family of Tyrosine Kinases, in Patients with Behcet's Disease.
Clin. Med. Res., June 1, 2006; 4(2): 147 - 151.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. R. Marshall, E. Olivas, S. Andreansky, N. L. La Gruta, G. A. Neale, A. Gutierrez, D. G. Wichlan, S. Wingo, C. Cheng, P. C. Doherty, et al.
Effector CD8+ T cells recovered from an influenza pneumonia differentiate to a state of focused gene expression
PNAS, April 26, 2005; 102(17): 6074 - 6079.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Lu, P. Zagouras, J. E. Fischer, J. Lu, B. Li, and R. A. Flavell
Kinetic analysis of genomewide gene expression reveals molecule circuitries that control T cell activation and Th1/2 differentiation
PNAS, March 2, 2004; 101(9): 3023 - 3028.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J.-z. Zhang, M. Sinha, B. A. Luxon, and X.-j. Yu
Survival Strategy of Obligately Intracellular Ehrlichia chaffeensis: Novel Modulation of Immune Response and Host Cell Cycles
Infect. Immun., January 1, 2004; 72(1): 498 - 507.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Z. Chen, R. Lund, T. Aittokallio, M. Kosonen, O. Nevalainen, and R. Lahesmaa
Identification of Novel IL-4/Stat6-Regulated Genes in T Lymphocytes
J. Immunol., October 1, 2003; 171(7): 3627 - 3635.
[Abstract] [Full Text] [PDF]

Home page
 JDRHome page
F. Carinci, F. Francioso, A. Piattelli, C. Rubini, M. Fioroni, R. Evangelisti, D. Arcelli, L. Tosi, F. Pezzetti, P. Carinci, et al.
Genetic Expression Profiling of Six Odontogenic Tumors
Journal of Dental Research, July 1, 2003; 82(7): 551 - 557.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Takesono, L. D. Finkelstein, and P. L. Schwartzberg
Beyond calcium: new signaling pathways for Tec family kinases
J. Cell Sci., January 8, 2002; 115(15): 3039 - 3048.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2002 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2002 by The American Association of Immunologists, Inc. All rights reserved.