| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
,
,,,
*
Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University, and
Departments of Medicine, Microbiology, and Immunology, Stanford University School of Medicine, Stanford, CA 94305; and
Veterans Affairs Palo Alto Health Care System, Palo Alto, CA 94304
The intestinal homing receptor,
4
7, helps target lymphocytes to Peyer’s
patches (PP) and intestinal lamina propria (ILP). We have previously
shown that protective immunity to rotavirus (RV), an intestinal
pathogen, resides in memory B cells expressing
47. In this study, using a novel FACS
assay, we have directly studied the phenotype of B cells that express
surface RV-specific Ig during the in vivo RV immune response. During
primary infection, RV-specific B cells first appear as large
IgD-B220low47-
and 47+ cells (presumptive
extrafollicular, Ab-secreting B cells), and then as large and small
IgD-B220high47-
cells (presumptive germinal center B cells). The appearance of B cells
with the phenotype of large
IgD-B220low47+
cells in PP and most notably in mesenteric lymph nodes coincides with
the emergence of RV-specific Ab-secreting cells (ASC) in the ILP. Thus,
these B lymphocytes are good candidates for the migratory population
giving rise to the RV-specific ASC in the ILP. RV-specific long-term
memory B cells preferentially accumulate in PP and express
47. Nine months after infection most
RV-specific IgA ASC are found in PP and ILP and at lower frequency in
bone marrow and spleen. This study is the first to follow changes in
tissue-specific homing receptor expression during Ag-specific B cell
development in response to a natural host, tissue-specific pathogen.
These results show that 47 is tightly
regulated during the Ag-specific B cell response to RV and is expressed
concurrently with the specific migration of memory and effector B cells
to intestinal tissues.
This article has been cited by other articles:
|
|
 |
|
  H. E. Mei, T. Yoshida, W. Sime, F. Hiepe, K. Thiele, R. A. Manz, A. Radbruch, and T. Dorner Blood-borne human plasma cells in steady state are derived from mucosal immune responses Blood, March 12, 2009; 113(11): 2461 - 2469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Chachu, D. W. Strong, A. D. LoBue, C. E. Wobus, R. S. Baric, and H. W. Virgin IV Antibody Is Critical for the Clearance of Murine Norovirus Infection J. Virol., July 1, 2008; 82(13): 6610 - 6617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. T. Pals, D. J. J. de Gorter, and M. Spaargaren Lymphoma dissemination: the other face of lymphocyte homing Blood, November 1, 2007; 110(9): 3102 - 3111. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Di Martino, C. Basset, A. Ogier, A. Charpilienne, D. Poncet, and E. Kohli Distribution and phenotype of rotavirus-specific B cells induced during the antigen-driven primary response to 2/6 virus-like particles administered by the intrarectal and the intranasal routes J. Leukoc. Biol., October 1, 2007; 82(4): 821 - 828. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Graham, J. A. O'Donnell, Y. Tan, N. Sanders, E. M. Carrington, J. Allison, and B. S. Coulson Rotavirus Infection of Infant and Young Adult Nonobese Diabetic Mice Involves Extraintestinal Spread and Delays Diabetes Onset J. Virol., June 15, 2007; 81(12): 6446 - 6458. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Feng, M. C. Jaimes, N. H. Lazarus, D. Monak, C. Zhang, E. C. Butcher, and H. B. Greenberg Redundant Role of Chemokines CCL25/TECK and CCL28/MEC in IgA+ Plasmablast Recruitment to the Intestinal Lamina Propria After Rotavirus Infection J. Immunol., May 15, 2006; 176(10): 5749 - 5759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Graham, F. E. Fleming, P. Halasz, M. J. Hewish, H. S. Nagesha, I. H. Holmes, Y. Takada, and B. S. Coulson Rotaviruses interact with {alpha}4{beta}7 and {alpha}4{beta}1 integrins by binding the same integrin domains as natural ligands J. Gen. Virol., December 1, 2005; 86(12): 3397 - 3408. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Valosky, H. Hishiki, T. E. Zaoutis, and S. E. Coffin Induction of Mucosal B-Cell Memory by Intranasal Immunization of Mice with Respiratory Syncytial Virus Clin. Vaccine Immunol., January 1, 2005; 12(1): 171 - 179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Parez, A. Garbarg-Chenon, C. Fourgeux, F. Le Deist, A. Servant-Delmas, A. Charpilienne, J. Cohen, and I. Schwartz-Cornil The VP6 Protein of Rotavirus Interacts with a Large Fraction of Human Naive B Cells via Surface Immunoglobulins J. Virol., November 15, 2004; 78(22): 12489 - 12496. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Jaimes, O. L. Rojas, E. J. Kunkel, N. H. Lazarus, D. Soler, E. C. Butcher, D. Bass, J. Angel, M. A. Franco, and H. B. Greenberg Maturation and Trafficking Markers on Rotavirus-Specific B Cells during Acute Infection and Convalescence in Children J. Virol., October 15, 2004; 78(20): 10967 - 10976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hieshima, Y. Kawasaki, H. Hanamoto, T. Nakayama, D. Nagakubo, A. Kanamaru, and O. Yoshie CC Chemokine Ligands 25 and 28 Play Essential Roles in Intestinal Extravasation of IgA Antibody-Secreting Cells J. Immunol., September 15, 2004; 173(6): 3668 - 3675. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. R. Vuillemenot, J. F. Rodriguez, and G. W. Hoyle Lymphoid Tissue and Emphysema in the Lungs of Transgenic Mice Inducibly Expressing Tumor Necrosis Factor-{alpha} Am. J. Respir. Cell Mol. Biol., April 1, 2004; 30(4): 438 - 448. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. H. Lazarus, E. J. Kunkel, B. Johnston, E. Wilson, K. R. Youngman, and E. C. Butcher A Common Mucosal Chemokine (Mucosae-Associated Epithelial Chemokine/CCL28) Selectively Attracts IgA Plasmablasts J. Immunol., April 1, 2003; 170(7): 3799 - 3805. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
