The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schroeijers, A. B.
Right arrow Articles by Scheper, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schroeijers, A. B.
Right arrow Articles by Scheper, R. J.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Hazardous Substances DB
*DAUNORUBICIN
The Journal of Immunology, 2002, 168: 1572-1578.
Copyright © 2002 by The American Association of Immunologists

Up-Regulation of Drug Resistance-Related Vaults During Dendritic Cell Development1

Anouk B. Schroeijers*, Anneke W. Reurs*, George L. Scheffer*, Anita G. M. Stam*, Mariska C. de Jong*, Thomas Rustemeyer*, Erik A. C. Wiemer{ddagger}, Tanja D. de Gruijl{dagger} and Rik J. Scheper2,*

Departments of * Pathology and {dagger} Medical Oncology, VU University Medical Center, Amsterdam, The Netherlands; and {ddagger} Department of Hematology, Erasmus University, Rotterdam, The Netherlands

P-glycoprotein (Pgp) and vaults are associated with multidrug resistance in tumor cells, but their physiological functions are not yet clear. Pgp, the prototypical transmembrane transporter molecule, may also facilitate the migration of skin dendritic cells (DC). Vaults—ribonucleoprotein cell organelles, frequently overexpressed in Pgp-negative drug-resistant tumor cells—have also been associated with intracellular transport processes. Given the pivotal role of DC in dealing with exposure to potentially harmful substances, the present study was set out to examine the expression of Pgp and vaults during differentiation and maturation of DC. DC were obtained from different sources, including blood-derived monocytes, CD34+ mononuclear cells, and chronic myeloid leukemia cells. Whereas flow cytometric and immunocytochemical analyses showed slightly augmented levels of Pgp, up-regulation of vault expression during DC culturing was strong, readily confirmed by Western blotting, and independent of the source of DC. In further exploring the functional significance of vault expression, it was found that supplementing DC cultures with polyclonal or mAbs against the major vault protein led to lower viabilities of LPS- or TNF-{alpha}-matured monocytes-DC. Moreover, expression of critical differentiation, maturation, and costimulatory molecules, including CD1a and CD83, was reduced and their capacity to induce Ag-specific T cell proliferative and IFN-{gamma} release responses was impaired. These data point to a role for vaults in both DC survival and functioning as APC.




This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
R. van de Ven, R. Oerlemans, J. W. van der Heijden, G. L. Scheffer, T. D. de Gruijl, G. Jansen, and R. J. Scheper
ABC drug transporters and immunity: novel therapeutic targets in autoimmunity and cancer
J. Leukoc. Biol., November 1, 2009; 86(5): 1075 - 1087.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
R. van de Ven, G. L. Scheffer, A. W. Reurs, J. J. Lindenberg, R. Oerlemans, G. Jansen, J.-P. Gillet, J. N. Glasgow, A. Pereboev, D. T. Curiel, et al.
A role for multidrug resistance protein 4 (MRP4; ABCC4) in human dendritic cell migration
Blood, September 15, 2008; 112(6): 2353 - 2359.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
M. Herlevsen, G. Oxford, C. R. Owens, M. Conaway, and D. Theodorescu
Depletion of major vault protein increases doxorubicin sensitivity and nuclear accumulation and disrupts its sequestration in lysosomes
Mol. Cancer Ther., June 1, 2007; 6(6): 1804 - 1813.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
K. A. Suprenant, N. Bloom, J. Fang, and G. Lushington
The major vault protein is related to the toxic anion resistance protein (TelA) family
J. Exp. Biol., March 15, 2007; 210(6): 946 - 955.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. van de Ven, M. C. de Jong, A. W. Reurs, A. J. N. Schoonderwoerd, G. Jansen, J. H. Hooijberg, G. L. Scheffer, T. D. de Gruijl, and R. J. Scheper
Dendritic Cells Require Multidrug Resistance Protein 1 (ABCC1) Transporter Activity for Differentiation
J. Immunol., May 1, 2006; 176(9): 5191 - 5198.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E. Steiner, K. Holzmann, C. Pirker, L. Elbling, M. Micksche, H. Sutterluty, and W. Berger
The major vault protein is responsive to and interferes with interferon-{gamma}-mediated STAT1 signals
J. Cell Sci., February 1, 2006; 119(3): 459 - 469.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. H. Mossink, A. van Zon, E. Franzel-Luiten, M. Schoester, V. A. Kickhoefer, G. L. Scheffer, R. J. Scheper, P. Sonneveld, and E. A. C. Wiemer
Disruption of the Murine Major Vault Protein (MVP/LRP) Gene Does Not Induce Hypersensitivity to Cytostatics
Cancer Res., December 15, 2002; 62(24): 7298 - 7304.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2002 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2002 by The American Association of Immunologists, Inc. All rights reserved.