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B Activity1
*
Institute for Nutrition Research and
Laboratory for Molecular Embryology, University of Oslo, Blindern, Oslo, Norway
A wide range of human disorders involves inappropriate regulation
of NF-
B, including cancers and numerous inflammatory conditions.
Toward our goal to define mechanisms through which NF-B leads to the
development of disease, we have developed transgenic mice that express
luciferase under the control of NF-B, enabling real-time in vivo
imaging of NF-B activity in intact animals. We show that in the
absence of extrinsic stimulation, strong luminescence is evident in
lymph nodes in the neck region, thymus, and Peyer’s patches. Treating
mice with TNF-
, IL-1, or LPS increased the luminescence in a
tissue-specific manner, with the strongest activity observed in skin,
lungs, spleen, Peyer’s patches, and the wall of the small intestine.
Liver, kidney, heart, muscle, and adipose tissue displayed less intense
activities. Also, exposure of skin to a low dose of UV radiation
increased luminescence in the exposed areas. Furthermore, induction of
chronic inflammation resembling rheumatoid arthritis produced strong
NF-B activity in the affected joints, as revealed by in vivo
imaging. Thus, we have developed a versatile model for monitoring
NF-B activation in vivo.
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