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The Journal of Immunology, 2002, 168: 1167-1171.
Copyright © 2002 by The American Association of Immunologists

Functional Avidity of Tumor Antigen-Specific CTL Recognition Directly Correlates with the Stability of MHC/Peptide Multimer Binding to TCR1

Valérie Dutoit*, Verena Rubio-Godoy*, Marie-Agnès Doucey1,{ddagger}, Pascal Batard*, Danielle Liénard*,{dagger}, Donata Rimoldi{ddagger}, Daniel Speiser*, Philippe Guillaume{ddagger}, Jean-Charles Cerottini*,{ddagger}, Pedro Romero* and Danila Valmori2,*

* Division of Clinical Onco-Immunology, Ludwig Institute for Cancer Research, and {dagger} Multidisciplinary Oncology Center, University Hospital, Lausanne, Switzerland; and {ddagger} Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, Epalinges, Switzerland

Avidity of Ag recognition by tumor-specific T cells is one of the main parameters that determines the potency of a tumor rejection Ag. In this study we show that the relative efficiency of staining of tumor Ag-specific T lymphocytes with the corresponding fluorescent MHC class I/peptide multimeric complexes can considerably vary with staining conditions and does not necessarily correlate with avidity of Ag recognition. Instead, we found a clear correlation between avidity of Ag recognition and the stability of MHC class I/peptide multimeric complexes interaction with TCR as measured in dissociation kinetic experiments. These findings are relevant for both identification and isolation of tumor-reactive CTL.




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