| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Laboratory of Molecular and Cellular Recognition, Osaka University Graduate School of Medicine; and
Institute for Molecular and Cellular Biology, Osaka University, Suita, Japan
High endothelial venule (HEV) cells support lymphocyte migration
from the peripheral blood into secondary lymphoid tissues. Using gene
expression profiling of mucosal addressin cell adhesion
molecule-1+ mesenteric lymph node HEV cells by quantitative
3'-cDNA collection, we have identified a leucine-rich protein, named
leucine-rich HEV glycoprotein (LRHG) that is selectively expressed in
these cells. Northern blot analysis revealed that LRHG mRNA is 
1.3
kb and is expressed in lymph nodes, liver, and heart. In situ
hybridization analysis demonstrated that the mRNA expression in lymph
nodes is strictly restricted to the HEV cells, and immunofluorescence
analysis with polyclonal Abs against LRHG indicated that the LRHG
protein is localized mainly to HEV cells and possibly to some lymphoid
cells surrounding the HEVs. LRHG cDNA encodes a 342-aa protein
containing 8 tandem leucine-rich repeats of 24 aa each and has high
homology to human leucine-rich 
2-glycoprotein. Similar
to some other leucine-rich repeat protein family members, LRHG can bind
extracellular matrix proteins that are expressed on the basal lamina of
HEVs, such as fibronectin, collagen IV, and laminin. In addition, LRHG
binds TGF-
. These results suggest that LRHG is likely to be
multifunctional in that it may capture TGF- and/or other related
humoral factors to modulate cell adhesion locally and may also be
involved in the adhesion of HEV cells to the surrounding basal
lamina.
This article has been cited by other articles:
|
|
 |
|
  T. Nakasaki, T. Tanaka, S. Okudaira, M. Hirosawa, E. Umemoto, K. Otani, S. Jin, Z. Bai, H. Hayasaka, Y. Fukui, et al. Involvement of the Lysophosphatidic Acid-Generating Enzyme Autotaxin in Lymphocyte-Endothelial Cell Interactions Am. J. Pathol., November 1, 2008; 173(5): 1566 - 1576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ai, L. J. Druhan, M. G. Hunter, M. J. Loveland, and B. R. Avalos LRG-accelerated differentiation defines unique G-CSFR signaling pathways downstream of PU.1 and C/EBP{epsilon} that modulate neutrophil activation J. Leukoc. Biol., May 1, 2008; 83(5): 1277 - 1285. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Umemoto, T. Tanaka, H. Kanda, S. Jin, K. Tohya, K. Otani, T. Matsutani, M. Matsumoto, Y. Ebisuno, M. H. Jang, et al. Nepmucin, a novel HEV sialomucin, mediates L-selectin-dependent lymphocyte rolling and promotes lymphocyte adhesion under flow J. Exp. Med., June 12, 2006; 203(6): 1603 - 1614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Norkina, T. G. Burnett, and R. C. De Lisle Bacterial Overgrowth in the Cystic Fibrosis Transmembrane Conductance Regulator Null Mouse Small Intestine Infect. Immun., October 1, 2004; 72(10): 6040 - 6049. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kanda, T. Tanaka, M. Matsumoto, E. Umemoto, Y. Ebisuno, M. Kinoshita, M. Noda, R. Kannagi, T. Hirata, T. Murai, et al. Endomucin, a sialomucin expressed in high endothelial venules, supports L-selectin-mediated rolling Int. Immunol., September 1, 2004; 16(9): 1265 - 1274. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-A. Lacorre, E. S. Baekkevold, I. Garrido, P. Brandtzaeg, G. Haraldsen, F. Amalric, and J.-P. Girard Plasticity of endothelial cells: rapid dedifferentiation of freshly isolated high endothelial venule endothelial cells outside the lymphoid tissue microenvironment Blood, June 1, 2004; 103(11): 4164 - 4172. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Palmeri, F.-R. Zuo, S. D. Rosen, and S. Hemmerich Differential gene expression profile of human tonsil high endothelial cells: implications for lymphocyte trafficking J. Leukoc. Biol., May 1, 2004; 75(5): 910 - 927. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kashiwazaki, T. Tanaka, H. Kanda, Y. Ebisuno, D. Izawa, N. Fukuma, N. Akimitsu, K. Sekimizu, M. Monden, and M. Miyasaka A high endothelial venule-expressing promiscuous chemokine receptor DARC can bind inflammatory, but not lymphoid, chemokines and is dispensable for lymphocyte homing under physiological conditions Int. Immunol., October 1, 2003; 15(10): 1219 - 1227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ebisuno, T. Tanaka, N. Kanemitsu, H. Kanda, K. Yamaguchi, T. Kaisho, S. Akira, and M. Miyasaka Cutting Edge: The B Cell Chemokine CXC Chemokine Ligand 13/B Lymphocyte Chemoattractant Is Expressed in the High Endothelial Venules of Lymph Nodes and Peyer's Patches and Affects B Cell Trafficking Across High Endothelial Venules J. Immunol., August 15, 2003; 171(4): 1642 - 1646. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Nagakubo, T. Murai, T. Tanaka, T. Usui, M. Matsumoto, K. Sekiguchi, and M. Miyasaka A High Endothelial Venule Secretory Protein, Mac25/Angiomodulin, Interacts with Multiple High Endothelial Venule-Associated Molecules Including Chemokines J. Immunol., July 15, 2003; 171(2): 553 - 561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Usui, T. Murai, T. Tanaka, K. Yamaguchi, D. Nagakubo, C. M. Lee, M. Kiyomi, S. Tamura, Y. Matsuzawa, and M. Miyasaka Characterization of mac25/angiomodulin expression by high endothelial venule cells in lymphoid tissues and its identification as an inducible marker for activated endothelial cells Int. Immunol., November 1, 2002; 14(11): 1273 - 1282. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
