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T Cells Stimulated with Soluble or Cellular Antigens1
Institut National de la Santé et de la Recherche Médicale, Centre Hospitalier Universitaire Purpan, Toulouse, France
B, 
T, and NK lymphocytes establish immunological
synapses (IS) with their targets to enable recognition. Transfer of
target cell-derived Ags together with proximal molecules onto the
effector cell appears also to occur through synapses. Little is known
about the molecular basis of this transfer, but it is assumed to result
from Ag receptor internalization. Because human 
T cells
recognize soluble nonpeptidic phosphoantigens as well as tumor cells
such as Daudi, it is unknown whether they establish IS with, and
extract molecules from, target cells. Using flow cytometry and confocal
microscopy, we show in this work that Ag-stimulated human V9/V2 T
cells conjugate to, and perform molecular transfer from, various tumor
cell targets. The molecular transfer appears to be linked to IS
establishment, evolves in a dose-dependent manner in the presence of
either soluble or cellular Ag, and requires TCR ligation, Src
family kinase signaling, and participation of the actin cytoskeleton.
Although CD45 exclusion characterized the IS performed by T
cells, no obvious capping of the TCR was detected. The synaptic
transfer mediated by T cells involved target molecules unrelated
to the cognate Ag and occurred independently of MHC class I expression
by target cells. From these observations, we conclude thatm despite the
particular features of T cell activation, both synapse formation
and molecular transfer of determinants belonging to target cell
characterize T cell recognition of Ags.
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