HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fortin, M. Articles by Hugo, P. Search for Related Content PubMed PubMed Citation Articles by Fortin, M. Articles by Hugo, P.

Apoptosis Mediated Through CD45 Is Independent of Its Phosphatase Activity and Association with Leukocyte Phosphatase-Associated Phosphoprotein¹

Marylène Fortin^*, Ann-Muriel Steff^*, Jackie Felberg, Isabelle Ding, Burkhart Schraven, Pauline Johnson and Patrice Hugo^2,*

^* Division of Research and Development, PROCREA BioSciences, Inc., Montréal, Québec, Canada; Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada; and Otto von Guericke Universität Magdeburg, Institute of Immunology, Magdeburg, Germany

Besides the well-recognized role of CD45 as a major player in TCR signaling, we and others have demonstrated that cross-linking of CD45 with mAbs can induce cell death in T lymphocytes. To investigate the role of CD45 phosphatase activity in apoptosis induction, we expressed either wild-type or phosphatase-dead CD45 molecules in a CD45-deficient BW5147 T cell line. We show here that the phosphatase activity of CD45 was not required for apoptosis triggering after cross-linking of the molecule. It is noteworthy that a revertant of the CD45-negative BW5147 cell line, expressing a truncated form of CD45 lacking most of the cytoplasmic domain, was also susceptible to CD45-mediated death. Moreover, we also demonstrate that leukocyte phosphatase-associated phosphoprotein expression is totally dispensable for CD45-mediated apoptosis to occur. Taken together, these results strongly suggest a role for the extracellular and/or the transmembrane portion of CD45 in apoptosis signaling, which contrasts with the previously reported functions for CD45 in T lymphocytes.

This article has been cited by other articles:

				Z. Liu, R. Dawes, S. Petrova, P. C. Beverley, and E. Z Tchilian CD45 regulates apoptosis in peripheral T lymphocytes Int. Immunol., June 1, 2006; 18(6): 959 - 966. [Abstract] [Full Text] [PDF]

				A. R. Anand and R. K. Ganju HIV-1 gp120-mediated Apoptosis of T Cells Is Regulated by the Membrane Tyrosine Phosphatase CD45 J. Biol. Chem., May 5, 2006; 281(18): 12289 - 12299. [Abstract] [Full Text] [PDF]

				S. Gregori, P. Mangia, R. Bacchetta, E. Tresoldi, F. Kolbinger, C. Traversari, J. M. Carballido, J. E. de Vries, U. Korthauer, and M.-G. Roncarolo An anti-CD45RO/RB monoclonal antibody modulates T cell responses via induction of apoptosis and generation of regulatory T cells J. Exp. Med., April 18, 2005; 201(8): 1293 - 1305. [Abstract] [Full Text] [PDF]

				C. Zhou, A. Saxon, and K. Zhang Human Activation-Induced Cytidine Deaminase Is Induced by IL-4 and Negatively Regulated by CD45: Implication of CD45 as a Janus Kinase Phosphatase in Antibody Diversification J. Immunol., February 15, 2003; 170(4): 1887 - 1893. [Abstract] [Full Text] [PDF]