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v
3 and v5 Integrins Are Not Essential for MHC Class I Cross-Presentation of Cell-Associated Antigen by CD8+ Murine Dendritic Cells1
* Immunobiology Laboratory,
Lymphocyte Molecular Biology Laboratory, and
Cell Adhesion and Disease Laboratory, Cancer Research UK, London Research Institute, London, United Kingdom; and
Division of Hematology and Medical Oncology, Department of Medicine, Weill Medical College of Cornell University, New York, NY 10021
Cross-presentation of cell-associated Ag is thought to involve
receptor-mediated uptake of apoptotic cells by dendritic cells (DC),
and studies with human DC strongly implicate the endocytic receptor
CD36 and the integrins 
v
3 and/or
v5 in this process. In the mouse,
cross-presentation was recently shown to be a function of
CD8+ DC. Here we report that CD36 is expressed on
CD8+, but not on CD8-, DC. To address
the role of CD36 in cross-presentation we compared
CD36-/- and CD36+/+ H-2b DC for
their ability to stimulate naive OT-1 T cells specific for OVA plus
H-2Kb in the presence of OVA-loaded MHC-mismatched
splenocytes as a source of cell-associated Ag for cross-presentation.
Surprisingly, no difference was seen between CD36-/- and
CD36+/+ CD8+ DC in their ability to
cross-present cell-associated OVA or to capture OVA-bearing cells.
Furthermore, the proliferation of CFSE-labeled OT-1 cells in response
to OVA cross-presentation in vivo was normal in CD36-/-
bone marrow chimeras, also arguing against a necessary role for CD36 in
cross-presentation by DC or other APC. DC doubly deficient for
3 and 5 integrins were similarly
unimpaired in their ability to cross-present OVA-bearing cells in
vitro. These data demonstrate that in the mouse, receptors other than
CD36 or 3 and 5 integrins can support the
specialized cross-presenting function of CD8+
DC.
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