The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Koralnik, I. J.
Right arrow Articles by Letvin, N. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koralnik, I. J.
Right arrow Articles by Letvin, N. L.
The Journal of Immunology, 2002, 168: 499-504.
Copyright © 2002 by The American Association of Immunologists

Association of Prolonged Survival in HLA-A2+ Progressive Multifocal Leukoencephalopathy Patients with a CTL Response Specific for a Commonly Recognized JC Virus Epitope1

Igor J. Koralnik2,*,{dagger}, Renaud A. Du Pasquier*,{dagger}, Marcelo J. Kuroda{dagger}, Jörn E. Schmitz{dagger}, Xin Dang{dagger}, Yue Zheng{dagger}, Michelle Lifton{dagger} and Norman L. Letvin{dagger}

* Neurology Department and {dagger} Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215

The role of JC virus (JCV)-specific CTL was explored in the immunopathogenesis of progressive multifocal leukoencephalopathy (PML). We identified a 9-aa epitope of the JCV capsid protein VP1, the VP1p100 peptide ILMWEAVTL, which is recognized by CTL of HLA-A2+ HIV+/PML survivors. We then constructed an HLA-A*0201/VP1p100 tetrameric complex that allowed us to assess by flow cytometry the PBMC of 13 PML patients and 11 control subjects for the presence of JCV-specific CTL. VP1p100-specific CTL were detected by tetramer binding in VP1p100-stimulated PBMC of five of seven (71%) PML survivors and zero of six PML progressors (p = 0.02). Two of three HIV+ patients with a leukoencephalopathy resembling PML, but with no virologic evidence of JCV infection, also had detectable VP1p100-specific CTL in their PBMC. PBMC of eight HIV+ patients with other neurologic diseases and healthy control subjects had no detectable JCV-specific CTL. These data suggest that the JCV-specific cellular immune response may be important in the containment of PML, and the tetramer-staining assay may provide a useful prognostic tool in the clinical management of these patients.




This article has been cited by other articles:


Home page
 NeurologyHome page
A. Marzocchetti, T. Tompkins, D. B. Clifford, R. T. Gandhi, S. Kesari, J. R. Berger, D. M. Simpson, M. Prosperi, A. De Luca, and I. J. Koralnik
Determinants of survival in progressive multifocal leukoencephalopathy
Neurology, November 10, 2009; 73(19): 1551 - 1558.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
Y. Chen, E. Bord, T. Tompkins, J. Miller, C. S. Tan, R. P. Kinkel, M. C. Stein, R. P. Viscidi, L. H. Ngo, and I. J. Koralnik
Asymptomatic Reactivation of JC Virus in Patients Treated with Natalizumab
N. Engl. J. Med., September 10, 2009; 361(11): 1067 - 1074.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
N. Khanna, M. Wolbers, N. J. Mueller, C. Garzoni, R. A. Du Pasquier, C. A. Fux, P. Vernazza, E. Bernasconi, R. Viscidi, M. Battegay, et al.
JC Virus-Specific Immune Responses in Human Immunodeficiency Virus Type 1 Patients with Progressive Multifocal Leukoencephalopathy
J. Virol., May 1, 2009; 83(9): 4404 - 4411.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
P. A. Swanson II, C. D. Pack, A. Hadley, C.-R. Wang, I. Stroynowski, P. E. Jensen, and A. E. Lukacher
An MHC class Ib-restricted CD8 T cell response confers antiviral immunity
J. Exp. Med., July 7, 2008; 205(7): 1647 - 1657.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
W Yang, E. Beaudoin, L Lu, R. Du Pasquier, M. Kuroda, R. Willemsen, I. Koralnik, and R. Junghans
Chimeric immune receptors (CIRs) specific to JC virus for immunotherapy in progressive multifocal leukoencephalopathy (PML)
Int. Immunol., September 1, 2007; 19(9): 1083 - 1093.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. A. Lima, A. Marzocchetti, P. Autissier, T. Tompkins, Y. Chen, J. Gordon, D. B. Clifford, R. T. Gandhi, N. Venna, J. R. Berger, et al.
Frequency and Phenotype of JC Virus-Specific CD8+ T Lymphocytes in the Peripheral Blood of Patients with Progressive Multifocal Leukoencephalopathy
J. Virol., April 1, 2007; 81(7): 3361 - 3368.
[Abstract] [Full Text] [PDF]

Home page
 Arch NeurolHome page
O. Stuve, C. M. Marra, P. D. Cravens, M. P. Singh, W. Hu, A. Lovett-Racke, N. L. Monson, J. T. Phillips, J. W. C. Tervaert, R. A. Nash, et al.
Potential Risk of Progressive Multifocal Leukoencephalopathy With Natalizumab Therapy: Possible Interventions
Arch Neurol, February 1, 2007; 64(2): 169 - 176.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
Y. Chen, J. Trofe, J. Gordon, R. A. Du Pasquier, P. Roy-Chaudhury, M. J. Kuroda, E. S. Woodle, K. Khalili, and I. J. Koralnik
Interplay of Cellular and Humoral Immune Responses against BK Virus in Kidney Transplant Recipients with Polyomavirus Nephropathy.
J. Virol., April 1, 2006; 80(7): 3495 - 3505.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
L. Krymskaya, M. C. Sharma, J. Martinez, W. Haq, E. C. Huang, A. P. Limaye, D. J. Diamond, and S. F. Lacey
Cross-Reactivity of T Lymphocytes Recognizing a Human Cytotoxic T-Lymphocyte Epitope within BK and JC Virus VP1 Polypeptides
J. Virol., September 1, 2005; 79(17): 11170 - 11178.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. A. Du Pasquier, J. E. Schmitz, J. Jean-Jacques, Y. Zheng, J. Gordon, K. Khalili, N. L. Letvin, and I. J. Koralnik
Detection of JC Virus-Specific Cytotoxic T Lymphocytes in Healthy Individuals
J. Virol., September 15, 2004; 78(18): 10206 - 10210.
[Abstract] [Full Text] [PDF]

Home page
 BrainHome page
R. A. Du Pasquier, M. J. Kuroda, Y. Zheng, J. Jean-Jacques, N. L. Letvin, and I. J. Koralnik
A prospective study demonstrates an association between JC virus-specific cytotoxic T lymphocytes and the early control of progressive multifocal leukoencephalopathy
Brain, September 1, 2004; 127(9): 1970 - 1978.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
I. J. Koralnik, D. Schellingerhout, and M. P. Frosch
Case 14-2004 - A 66-Year-Old Man with Progressive Neurologic Deficits
N. Engl. J. Med., April 29, 2004; 350(18): 1882 - 1893.
[Full Text] [PDF]

Home page
 J. Virol.Home page
R. A. Du Pasquier, M. J. Kuroda, J. E. Schmitz, Y. Zheng, K. Martin, F. W. Peyerl, M. Lifton, D. Gorgone, P. Autissier, N. L. Letvin, et al.
Low Frequency of Cytotoxic T Lymphocytes against the Novel HLA-A*0201-Restricted JC Virus Epitope VP1p36 in Patients with Proven or Possible Progressive Multifocal Leukoencephalopathy
J. Virol., November 15, 2003; 77(22): 11918 - 11926.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2002 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2002 by The American Association of Immunologists, Inc. All rights reserved.