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ICA69^null Nonobese Diabetic Mice Develop Diabetes, but Resist Disease Acceleration by Cyclophosphamide¹

Shawn Winer^2,*, Igor Astsaturov^2,*, Roger Gaedigk^2,*, Denise Hammond-McKibben^*, Marc Pilon^*, Aihua Song^*, Violetta Kubiak^*, Wolfram Karges^*, Enrico Arpaia^*, Colin McKerlie, Peter Zucker, Bhagirath Singh and H.-Michael Dosch^3,*

^* The Hospital For Sick Children, Research Institute, University of Toronto, Toronto, Ontario, Canada; Departments of Pediatrics and Immunology, Sunnybrook and Women’s College Health Sciences Center, and University of Western Ontario, Department of Immunology, John P. Robarts Research Institute, London, Ontario, Canada

ICA69 (islet cell Ag 69 kDa) is a diabetes-associated autoantigen with high expression levels in cells and brain. Its function is unknown, but knockout of its Caenorhabditis elegans homologue, ric-19, compromised neurotransmission. We disrupted the murine gene, ica-1, in 129-strain mice. These animals aged normally, but speed-congenic ICA69^null nonobese diabetic (NOD) mice developed mid-life lethality, reminiscent of NOD-specific, late lethal seizures in glutamic acid decarboxylase 65-deficient mice. In contrast to wild-type and heterozygous animals, ICA69^null NOD congenics fail to generate, even after immunization, cross-reactive T cells that recognize the dominant Tep69 epitope in ICA69, and its environmental mimicry Ag, the ABBOS epitope in BSA. This antigenic mimicry is thus driven by the endogenous self Ag, and not initiated by the environmental mimic. Insulitis, spontaneous, and adoptively transferred diabetes develop normally in ICA69^null NOD congenics. Like glutamic acid decarboxylase 65, ICA69 is not an obligate autoantigen in diabetes. Unexpectedly, ICA69^null NOD mice were resistant to cyclophosphamide (CY)-accelerated diabetes. Transplantation experiments with hemopoietic and islet tissue linked CY resistance to ICA69 deficiency in islets. CY-accelerated diabetes involves not only ablation of lymphoid cells, but ICA69-dependent drug toxicity in cells that boosts autoreactivity in the regenerating lymphoid system.

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