| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
and Cyclic Adenosine 5'-Monophosphate Response Element Binding Protein1
Centre de Recherche en Infectiologie, Hôpital CHUL, Centre Hospitalier Universitaire de Québec, and Département de Biologie Médicale, Faculté de Médecine, Université Laval, Ste-Foy, Québec, Canada
Previous work indicates that treatment of human T cells with
PGE2 results in an increase of HIV-1 long terminal repeat
(LTR) transcriptional activity. The noticed PGE2-mediated
activation of virus gene activity required the participation of
specific intracellular second messengers such as calcium and two
transcription factors, i.e., NF-
B and CREB. We report in this work
that the nuclear transcription factor CCAAT/enhancer binding protein
(C/EBP) is also important for PGE2-dependent up-regulation
of HIV-1 LTR-driven gene activity. The implication of C/EBP was shown
by using a trans-dominant negative inhibitor of C/EBP
(i.e., liver-enriched transcriptional inhibitory protein) and
several molecular constructs carrying site-directed mutations in the
C/EBP binding sites located within the HIV-1 LTR. Mutated HIV-1 LTR
constructs also revealed the involvement of the two most proximal C/EBP
binding sites. Data from cotransfection experiments with vectors coding
for dominant negative mutants and gel mobility shift assays indicated
that PGE2-mediated induction of HIV-1 LTR activity results
from a cooperative interaction between C/EBP
and CREB, two members
of the basic leucine zipper family of transcription factors. Altogether
these findings indicate that treatment of human T cells with
PGE2 induces HIV-1 LTR activity through a complex interplay
between C/EBP and CREB. Such a combinatorial regulation may
represent a mechanism that permits a fine regulation of HIV-1
expression by PGE2 in human T cells.
This article has been cited by other articles:
|
|
 |
|
  T. Khayrullina, J.-H. Yen, H. Jing, and D. Ganea In Vitro Differentiation of Dendritic Cells in the Presence of Prostaglandin E2 Alters the IL-12/IL-23 Balance and Promotes Differentiation of Th17 Cells J. Immunol., July 1, 2008; 181(1): 721 - 735. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Karamanlidis, A. Karamitri, K. Docherty, D. G. Hazlerigg, and M. A. Lomax C/EBPbeta Reprograms White 3T3-L1 Preadipocytes to a Brown Adipocyte Pattern of Gene Expression J. Biol. Chem., August 24, 2007; 282(34): 24660 - 24669. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Alfranca, M. A. Iniguez, M. Fresno, and J. M. Redondo Prostanoid signal transduction and gene expression in the endothelium: Role in cardiovascular diseases Cardiovasc Res, June 1, 2006; 70(3): 446 - 456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Karaya, S. Mori, H. Kimoto, Y. Shima, Y. Tsuji, H. Kurooka, S. Akira, and Y. Yokota Regulation of Id2 expression by CCAAT/enhancer binding protein {beta} Nucleic Acids Res., April 4, 2005; 33(6): 1924 - 1934. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. D. Burkart, A. Mukherjee, E. Sterneck, P. F. Johnson, and K. E. Mayo Repression of the Inhibin {alpha}-Subunit Gene by the Transcription Factor CCAAT/Enhancer-Binding Protein-{beta} Endocrinology, April 1, 2005; 146(4): 1909 - 1921. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Moriuchi and H. Moriuchi Cell-Type-Dependent Effect of Transforming Growth Factor {beta}, a Major Cytokine in Breast Milk, on Human Immunodeficiency Virus Type 1 Infection of Mammary Epithelial MCF-7 Cells or Macrophages J. Virol., December 1, 2004; 78(23): 13046 - 13052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gerlo, P. Verdood, B. Gellersen, E. L. Hooghe-Peters, and R. Kooijman Mechanism of Prostaglandin (PG)E2-Induced Prolactin Expression in Human T Cells: Cooperation of Two PGE2 Receptor Subtypes, E-Prostanoid (EP) 3 and EP4, Via Calcium- and Cyclic Adenosine 5'-Monophosphate-Mediated Signaling Pathways J. Immunol., November 15, 2004; 173(10): 5952 - 5962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. A. Rossen, J. Bouma, R. H. C. Raatgeep, H. A. Buller, and A. W. C. Einerhand Inhibition of Cyclooxygenase Activity Reduces Rotavirus Infection at a Postbinding Step J. Virol., September 15, 2004; 78(18): 9721 - 9730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Symensma, D. Martinez-Guzman, Q. Jia, E. Bortz, T.-T. Wu, N. Rudra-Ganguly, S. Cole, H. Herschman, and R. Sun COX-2 Induction during Murine Gammaherpesvirus 68 Infection Leads to Enhancement of Viral Gene Expression J. Virol., December 1, 2003; 77(23): 12753 - 12763. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Rohr, C. Marban, D. Aunis, and E. Schaeffer Regulation of HIV-1 gene transcription: from lymphocytes to microglial cells J. Leukoc. Biol., November 1, 2003; 74(5): 736 - 749. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Dumais, M.-E. Pare, S. Mercier, S. Bounou, S. J. Marriot, B. Barbeau, and M. J. Tremblay T-Cell Receptor/CD28 Engagement When Combined with Prostaglandin E2 Treatment Leads to Potent Activation of Human T-Cell Leukemia Virus Type 1 J. Virol., October 15, 2003; 77(20): 11170 - 11179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. S. Sun, K.-Y. Hsiao, C.-C. Hsu, M.-H. Wu, and S.-J. Tsai Transactivation of Steroidogenic Acute Regulatory Protein in Human Endometriotic Stromal Cells Is Mediated by the Prostaglandin EP2 Receptor Endocrinology, September 1, 2003; 144(9): 3934 - 3942. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Chen, S. Zhuang, S. Cassenaer, D. E. Casteel, T. Gudi, G. R. Boss, and R. B. Pilz Synergism between Calcium and Cyclic GMP in Cyclic AMP Response Element-Dependent Transcriptional Regulation Requires Cooperation between CREB and C/EBP-{beta} Mol. Cell. Biol., June 15, 2003; 23(12): 4066 - 4082. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hannenhalli and S. Levy Predicting transcription factor synergism Nucleic Acids Res., October 1, 2002; 30(19): 4278 - 4284. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
