| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
,
Departments of
*
Microbiology and Molecular Genetics and
Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824; and
BioCentrum-DTU, Section for Biochemistry and Nutrition, Technical University of Denmark, Lyngby, Denmark
Dendritic cells (DC) play a pivotal immunoregulatory role in the
Th1, Th2, and Th3 cell balance and are present throughout the
gastrointestinal tract. Thus, DC may be targets for modulation by gut
microbes, including ingested probiotics. In the present study, we
tested the hypothesis that species of Lactobacillus,
important members of the gut flora, differentially activate DC. Bone
marrow-derived murine DC were exposed to various lethally irradiated
Lactobacillus spp. and resultant culture supernatants
were analyzed for IL-6, IL-10, IL-12, and TNF-
. Substantial
differences were found among strains in the capacity to induce IL-12
and TNF- production in the DC. Similar but less pronounced
differences were observed among lactobacilli in the induction of IL-6
and IL-10. Although all strains up-regulated surface MHC class II and
B7-2 (CD86), which is indicative of DC maturation, those lactobacilli
with greatest capacity to induce IL-12 were most effective. Remarkably,
Lactobacillus reuteri DSM12246, a poor IL-12 inducer,
inhibited IL-12, IL-6, and TNF- induction by the otherwise strong
cytokine inducer L. casei CHCC3139, while IL-10
production remained unaltered. In analogous fashion, L.
reuteri reduced L. casei-induced up-regulation
of B7-2. These results suggest that different species of
Lactobacillus exert very different DC activation
patterns and, furthermore, at least one species may be capable of
inhibiting activities of other species in the genus. Thus, the
potential exists for Th1/Th2/Th3-driving capacities of the gut DC to be
modulated according to composition of gut microflora, including
ingested probiotics.
This article has been cited by other articles:
|
|
 |
|
  V. Janardhana, M. M. Broadway, M. P. Bruce, J. W. Lowenthal, M. S. Geier, R. J. Hughes, and A. G. D. Bean Prebiotics Modulate Immune Responses in the Gut-Associated Lymphoid Tissue of Chickens J. Nutr., July 1, 2009; 139(7): 1404 - 1409. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mohamadzadeh, T. Duong, S. J. Sandwick, T. Hoover, and T. R. Klaenhammer Dendritic cell targeting of Bacillus anthracis protective antigen expressed by Lactobacillus acidophilus protects mice from lethal challenge PNAS, March 17, 2009; 106(11): 4331 - 4336. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Lebeer, J. Vanderleyden, and S. C. J. De Keersmaecker Genes and Molecules of Lactobacilli Supporting Probiotic Action Microbiol. Mol. Biol. Rev., December 1, 2008; 72(4): 728 - 764. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Walter Ecological Role of Lactobacilli in the Gastrointestinal Tract: Implications for Fundamental and Biomedical Research Appl. Envir. Microbiol., August 15, 2008; 74(16): 4985 - 4996. [Full Text] [PDF]
|
|
|
|
 |
|
 M. Li, X. Zhang, X. Zheng, D. Lian, Z.-X. Zhang, H. Sun, M. Suzuki, C. Vladau, X. Huang, X. Xia, et al. Tolerogenic dendritic cells transferring hyporesponsiveness and synergizing T regulatory cells in transplant tolerance Int. Immunol., February 1, 2008; 20(2): 285 - 293. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. L. Clancy and G. Pang Probiotics - Industry Myth or a Practical Reality? J. Am. Coll. Nutr., December 1, 2007; 26(6): 691S - 694S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. N. Fink, L. H. Zeuthen, H. R. Christensen, B. Morandi, H. Frokiaer, and G. Ferlazzo Distinct gut-derived lactic acid bacteria elicit divergent dendritic cell-mediated NK cell responses Int. Immunol., December 1, 2007; 19(12): 1319 - 1327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Cogan, T. P. Beresford, J. Steele, J. Broadbent, N. P. Shah, and Z. Ustunol Invited Review: Advances in Starter Cultures and Cultured Foods J Dairy Sci, September 1, 2007; 90(9): 4005 - 4021. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Isakow, L. E. Morrow, and M. H. Kollef Probiotics for Preventing and Treating Nosocomial Infections: Review of Current Evidence and Recommendations Chest, July 1, 2007; 132(1): 286 - 294. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Li, X. Zhang, X. Zheng, D. Lian, Z.-X. Zhang, W. Ge, J. Yang, C. Vladau, M. Suzuki, D. Chen, et al. Immune Modulation and Tolerance Induction by RelB-Silenced Dendritic Cells through RNA Interference J. Immunol., May 1, 2007; 178(9): 5480 - 5487. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Forsythe, M. D. Inman, and J. Bienenstock Oral Treatment with Live Lactobacillus reuteri Inhibits the Allergic Airway Response in Mice Am. J. Respir. Crit. Care Med., March 15, 2007; 175(6): 561 - 569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Winkler, D. Ghadimi, J. Schrezenmeir, and J.-P. Kraehenbuhl Molecular and Cellular Basis of Microflora-Host Interactions J. Nutr., March 1, 2007; 137(3): 756S - 772S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Corthesy, H. R. Gaskins, and A. Mercenier Cross-Talk between Probiotic Bacteria and the Host Immune System J. Nutr., March 1, 2007; 137(3): 781S - 790S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Savino, E. Pelle, E. Palumeri, R. Oggero, and R. Miniero Lactobacillus reuteri (American Type Culture Collection Strain 55730) Versus Simethicone in the Treatment of Infantile Colic: A Prospective Randomized Study Pediatrics, January 1, 2007; 119(1): e124 - e130. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Shida, T. Suzuki, J. Kiyoshima-Shibata, S.-i. Shimada, and M. Nanno Essential Roles of Monocytes in Stimulating Human Peripheral Blood Mononuclear Cells with Lactobacillus casei To Produce Cytokines and Augment Natural Killer Cell Activity. Clin. Vaccine Immunol., September 1, 2006; 13(9): 997 - 1003. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Shida, J. Kiyoshima-Shibata, M. Nagaoka, K. Watanabe, and M. Nanno Induction of interleukin-12 by lactobacillus strains having a rigid cell wall resistant to intracellular digestion. J Dairy Sci, September 1, 2006; 89(9): 3306 - 3317. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J Boyle, R. M Robins-Browne, and M. L. Tang Probiotic use in clinical practice: what are the risks? Am. J. Clinical Nutrition, June 1, 2006; 83(6): 1256 - 1264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R L Clancy, M Gleeson, A Cox, R Callister, M Dorrington, C D'Este, G Pang, D Pyne, P Fricker, and A Henriksson Reversal in fatigued athletes of a defect in interferon {gamma} secretion after administration of Lactobacillus acidophilus. Br. J. Sports Med., April 1, 2006; 40(4): 351 - 354. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. O'Mahony, L. O'Callaghan, J. McCarthy, D. Shilling, P. Scully, S. Sibartie, E. Kavanagh, W. O. Kirwan, H. P. Redmond, J. K. Collins, et al. Differential cytokine response from dendritic cells to commensal and pathogenic bacteria in different lymphoid compartments in humans Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G839 - G845. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. H. Zeuthen, H. R. Christensen, and H. Frokiaer Lactic Acid Bacteria Inducing a Weak Interleukin-12 and Tumor Necrosis Factor Alpha Response in Human Dendritic Cells Inhibit Strongly Stimulating Lactic Acid Bacteria but Act Synergistically with Gram-Negative Bacteria. Clin. Vaccine Immunol., March 1, 2006; 13(3): 365 - 375. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. B. Flohe, H. Agrawal, D. Schmitz, M. Gertz, S. Flohe, and F. U. Schade Dendritic cells during polymicrobial sepsis rapidly mature but fail to initiate a protective Th1-type immune response J. Leukoc. Biol., March 1, 2006; 79(3): 473 - 481. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-T. Tien, S. E. Girardin, B. Regnault, L. Le Bourhis, M.-A. Dillies, J.-Y. Coppee, R. Bourdet-Sicard, P. J. Sansonetti, and T. Pedron Anti-Inflammatory Effect of Lactobacillus casei on Shigella-Infected Human Intestinal Epithelial Cells J. Immunol., January 15, 2006; 176(2): 1228 - 1237. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. R. Haghighi, J. Gong, C. L. Gyles, M. A. Hayes, B. Sanei, P. Parvizi, H. Gisavi, J. R. Chambers, and S. Sharif Modulation of Antibody-Mediated Immune Response by Probiotics in Chickens Clin. Vaccine Immunol., December 1, 2005; 12(12): 1387 - 1392. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. I. Haza, A. Zabala, N. Arranz, and P. Morales Protective Effect of a Lactobacillus salivarius Strain of Human Origin Food Science and Technology International, August 1, 2005; 11(4): 251 - 259. [Abstract] [PDF]
|
|
|
|
|
|
C. Grangette, S. Nutten, E. Palumbo, S. Morath, C. Hermann, J. Dewulf, B. Pot, T. Hartung, P. Hols, and A. Mercenier From The Cover: Enhanced antiinflammatory capacity of a Lactobacillus plantarum mutant synthesizing modified teichoic acids PNAS, July 19, 2005; 102(29): 10321 - 10326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Verginis, H. S. Li, and G. Carayanniotis Tolerogenic Semimature Dendritic Cells Suppress Experimental Autoimmune Thyroiditis by Activation of Thyroglobulin-Specific CD4+CD25+ T Cells J. Immunol., June 1, 2005; 174(11): 7433 - 7439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Salminen, M. Gueimonde, and E. Isolauri Probiotics That Modify Disease Risk J. Nutr., May 1, 2005; 135(5): 1294 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Rochman, W. E. Paul, and S. Z. Ben-Sasson IL-6 Increases Primed Cell Expansion and Survival J. Immunol., April 15, 2005; 174(8): 4761 - 4767. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G A W Rook and L R Brunet Microbes, immunoregulation, and the gut Gut, March 1, 2005; 54(3): 317 - 320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Shanahan Physiological Basis for Novel Drug Therapies Used to Treat the Inflammatory Bowel Diseases I. Pathophysiological basis and prospects for probiotic therapy in inflammatory bowel disease Am J Physiol Gastrointest Liver Physiol, March 1, 2005; 288(3): G417 - G421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mohamadzadeh, S. Olson, W. V. Kalina, G. Ruthel, G. L. Demmin, K. L. Warfield, S. Bavari, and T. R. Klaenhammer Lactobacilli activate human dendritic cells that skew T cells toward T helper 1 polarization PNAS, February 22, 2005; 102(8): 2880 - 2885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Pena, A. B. Rogers, Z. Ge, V. Ng, S. Y. Li, J. G. Fox, and J. Versalovic Probiotic Lactobacillus spp. Diminish Helicobacter hepaticus-Induced Inflammatory Bowel Disease in Interleukin-10-Deficient Mice Infect. Immun., February 1, 2005; 73(2): 912 - 920. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A L Hart, K Lammers, P Brigidi, B Vitali, F Rizzello, P Gionchetti, M Campieri, M A Kamm, S C Knight, and A J Stagg Modulation of human dendritic cell phenotype and function by probiotic bacteria Gut, November 1, 2004; 53(11): 1602 - 1609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Young, M. A. Simon, M. A. Baird, G. W. Tannock, R. Bibiloni, K. Spencely, J. M. Lane, P. Fitzharris, J. Crane, I. Town, et al. Bifidobacterial Species Differentially Affect Expression of Cell Surface Markers and Cytokines of Dendritic Cells Harvested from Cord Blood Clin. Vaccine Immunol., July 1, 2004; 11(4): 686 - 690. [Abstract] [Full Text]
|
|
|
|
|
|
M. Drakes, T. Blanchard, and S. Czinn Bacterial Probiotic Modulation of Dendritic Cells Infect. Immun., June 1, 2004; 72(6): 3299 - 3309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Robinson, L. M. Chamberlain, M. C. Lopez, C. M. Rush, H. Marcotte, R. W. F. Le Page, and J. M. Wells Mucosal and Cellular Immune Responses Elicited by Recombinant Lactococcus lactis Strains Expressing Tetanus Toxin Fragment C Infect. Immun., May 1, 2004; 72(5): 2753 - 2761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S Ghosh, D van Heel, and R J Playford Probiotics in inflammatory bowel disease: is it all gut flora modulation? Gut, May 1, 2004; 53(5): 620 - 622. [Full Text] [PDF]
|
|
|
|
|
|
B Sheil, J McCarthy, L O'Mahony, M W Bennett, P Ryan, J J Fitzgibbon, B Kiely, J K Collins, and F Shanahan Is the mucosal route of administration essential for probiotic function? Subcutaneous administration is associated with attenuation of murine colitis and arthritis Gut, May 1, 2004; 53(5): 694 - 700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Veckman, M. Miettinen, J. Pirhonen, J. Siren, S. Matikainen, and I. Julkunen Streptococcus pyogenes and Lactobacillus rhamnosus differentially induce maturation and production of Th1-type cytokines and chemokines in human monocyte-derived dendritic cells J. Leukoc. Biol., May 1, 2004; 75(5): 764 - 771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Valeur, P. Engel, N. Carbajal, E. Connolly, and K. Ladefoged Colonization and Immunomodulation by Lactobacillus reuteri ATCC 55730 in the Human Gastrointestinal Tract Appl. Envir. Microbiol., February 1, 2004; 70(2): 1176 - 1181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Pena, S. Y. Li, P. H. Wilson, S. A. Thibodeau, A. J. Szary, and J. Versalovic Genotypic and Phenotypic Studies of Murine Intestinal Lactobacilli: Species Differences in Mice with and without Colitis Appl. Envir. Microbiol., January 1, 2004; 70(1): 558 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Reid, J. Jass, M. T. Sebulsky, and J. K. McCormick Potential Uses of Probiotics in Clinical Practice Clin. Microbiol. Rev., October 1, 2003; 16(4): 658 - 672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Benyacoub, G. L. Czarnecki-Maulden, C. Cavadini, T. Sauthier, R. E. Anderson, E. J. Schiffrin, and T. von der Weid Supplementation of Food with Enterococcus faecium (SF68) Stimulates Immune Functions in Young Dogs J. Nutr., April 1, 2003; 133(4): 1158 - 1162. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. R. Krehbiel, S. R. Rust, G. Zhang, and S. E. Gilliland Bacterial direct-fed microbials in ruminant diets: Performance response and mode of action J Anim Sci, February 1, 2003; 81(14_suppl_2): E120 - 132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. de Waard, E. Claassen, G. C. A. M. Bokken, B. Buiting, J. Garssen, and J. G. Vos Enhanced Immunological Memory Responses to Listeria monocytogenes in Rodents, as Measured by Delayed-Type Hypersensitivity (DTH), Adoptive Transfer of DTH, and Protective Immunity, following Lactobacillus casei Shirota Ingestion Clin. Vaccine Immunol., January 1, 2003; 10(1): 59 - 65. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ibnou-Zekri, S. Blum, E. J. Schiffrin, and T. von der Weid Divergent Patterns of Colonization and Immune Response Elicited from Two Intestinal Lactobacillus Strains That Display Similar Properties In Vitro Infect. Immun., January 1, 2003; 71(1): 428 - 436. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
