HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brown, K. L. Articles by Johnson, P. Search for Related Content PubMed PubMed Citation Articles by Brown, K. L. Articles by Johnson, P.

Role of Sulfation in CD44-Mediated Hyaluronan Binding Induced by Inflammatory Mediators in Human CD14⁺ Peripheral Blood Monocytes¹

Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada

Activation of T cells by Ag or stimulation of monocytes with inflammatory cytokines induces CD44 to bind to hyaluronan (HA), an adhesion event implicated in leukocyte-leukocyte, leukocyte-endothelial cell, and leukocyte-stromal cell interactions. We have previously shown that TNF- induces CD44 sulfation in a leukemic cell line, which correlated with the induction of HA binding and CD44-mediated adhesion. In this study, we establish that TNF- and IFN- induce HA binding and the sulfation of CD44 in CD14⁺ PBMC, whereas no induced HA binding or CD44 sulfation was observed in CD14^- PBMC stimulated with TNF-. Treatment of cells with NaClO₃, an inhibitor of sulfation, prevented HA binding in a significant percentage of CD14⁺ PBMC induced by TNF-, LPS, IL-1, or IFN-. Furthermore, stimulation with TNF- or IFN- in the presence of NaClO₃ reduced the ability of isolated CD44H to bind HA, demonstrating a direct effect of CD44H sulfation on HA binding. In contrast, the transient induction of HA binding in T cells by PHA was not affected by NaClO₃, suggesting that activated T cells do not use sulfation as a mechanism to regulate HA binding. Overall, these results demonstrate that inducible sulfation of CD44H is one mechanism used by CD14⁺ peripheral blood monocytes to induce HA binding in response to inflammatory agents such as TNF- and IFN-.

This article has been cited by other articles:

				H. Yamawaki, S. Hirohata, T. Miyoshi, K. Takahashi, H. Ogawa, R. Shinohata, K. Demircan, S. Kusachi, K. Yamamoto, and Y. Ninomiya Hyaluronan receptors involved in cytokine induction in monocytes Glycobiology, January 1, 2009; 19(1): 83 - 92. [Abstract] [Full Text] [PDF]

				G.-Y. Chen, K. Sakuma, and R. Kannagi Significance of NF-{kappa}B/GATA Axis in Tumor Necrosis Factor-{alpha}-induced Expression of 6-Sulfated Cell Recognition Glycans in Human T-lymphocytes J. Biol. Chem., December 12, 2008; 283(50): 34563 - 34570. [Abstract] [Full Text] [PDF]

				L. Zhao, J. A. Hall, N. Levenkova, E. Lee, M. K. Middleton, A. M. Zukas, D. J. Rader, J. J. Rux, and E. Pure CD44 Regulates Vascular Gene Expression in a Proatherogenic Environment Arterioscler Thromb Vasc Biol, April 1, 2007; 27(4): 886 - 892. [Abstract] [Full Text] [PDF]

				L. Zhuo, A. Kanamori, R. Kannagi, N. Itano, J. Wu, M. Hamaguchi, N. Ishiguro, and K. Kimata SHAP Potentiates the CD44-mediated Leukocyte Adhesion to the Hyaluronan Substratum J. Biol. Chem., July 21, 2006; 281(29): 20303 - 20314. [Abstract] [Full Text] [PDF]

				C. S. Bonder, S. R. Clark, M. U. Norman, P. Johnson, and P. Kubes Use of CD44 by CD4+ Th1 and Th2 lymphocytes to roll and adhere Blood, June 15, 2006; 107(12): 4798 - 4806. [Abstract] [Full Text] [PDF]

				S. L. Tjew, K. L. Brown, R. Kannagi, and P. Johnson Expression of N-acetylglucosamine 6-O-sulfotransferases (GlcNAc6STs)-1 and -4 in human monocytes: GlcNAc6ST-1 is implicated in the generation of the 6-sulfo N-acetyllactosamine/Lewis x epitope on CD44 and is induced by TNF-{alpha} Glycobiology, July 1, 2005; 15(7): 7C - 13C. [Abstract] [Full Text] [PDF]

				J Grisar, M Aringer, M D Koller, G H Stummvoll, D Eselbock, B Zwolfer, C W Steiner, B Zierhut, L Wagner, P Pietschmann, et al. Leflunomide inhibits transendothelial migration of peripheral blood mononuclear cells Ann Rheum Dis, December 1, 2004; 63(12): 1632 - 1637. [Abstract] [Full Text] [PDF]

				A. Krettek, G. K. Sukhova, U. Schonbeck, and P. Libby Enhanced Expression of CD44 Variants in Human Atheroma and Abdominal Aortic Aneurysm: Possible Role for a Feedback Loop in Endothelial Cells Am. J. Pathol., November 1, 2004; 165(5): 1571 - 1581. [Abstract] [Full Text] [PDF]

				K. Gee, M. Kozlowski, and A. Kumar Tumor Necrosis Factor-{alpha} Induces Functionally Active Hyaluronan-adhesive CD44 by Activating Sialidase through p38 Mitogen-activated Protein Kinase in Lipopolysaccharide-stimulated Human Monocytic Cells J. Biol. Chem., September 26, 2003; 278(39): 37275 - 37287. [Abstract] [Full Text] [PDF]

				J. Cichy and E. Pure The liberation of CD44 J. Cell Biol., June 9, 2003; 161(5): 839 - 843. [Abstract] [Full Text] [PDF]

				J. K. Lee, A. Bistrup, A. Van Zante, and S. D. Rosen Activities and expression pattern of the carbohydrate sulfotransferase GlcNAc6ST-3 (I-GlcNAc6ST): functional implications Glycobiology, April 1, 2003; 13(4): 245 - 254. [Abstract] [Full Text] [PDF]

				M. Delcommenne, R. Kannagi, and P. Johnson TNF-{alpha} increases the carbohydrate sulfation of CD44: induction of 6-sulfo N-acetyl lactosamine on N- and O-linked glycans Glycobiology, October 1, 2002; 12(10): 613 - 622. [Abstract] [Full Text] [PDF]