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Stability of Surface H-2K^b, H-2D^b, and Peptide-Receptive H-2K^b on Splenocytes¹

Ontario Cancer Institute, Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada

We have used flow cytometry to study the stability and peptide-binding capability of MHC class I (MHC-I) on the surface of normal C57BL/6 mouse T lymphoblasts. The MHC-I molecules on each cell are nearly evenly divided into two populations with mean half-life values of 1 and 20 h. Our observations suggest that members of the later contain peptide bound with medium to high affinity. Cell surface MHC-I molecules capable of binding exogenous peptide (thus, "peptide-receptive") belong almost entirely to the less stable population. Before exogenous peptide can bind, MHC-I must undergo a change, probably loss of a very low affinity peptide. For MHC-I-K^b, we found that the maximum rate for binding of exogenous peptide corresponds to a t_1/2 value of 12 min. To maintain the 50:50 steady-state distribution of long- vs short-lived MHC-I molecules on the cell surface, 20 short-lived molecules must be exported to the cell surface for each long-lived molecule.

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