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Cutting Edge |
Dermatology Branch, National Cancer Institute, Bethesda, MD 20892
The binding of a T cell to an Ag-laden dendritic cell (DC) is a
critical step of the acquired immune response. Herein, we address
whether a DC-produced chemokine can induce the arrest of T cells on DC
under dynamic flow conditions. Ag-primed T cells and a T cell line were
observed to rapidly (
0.5 s) bind to immobilized DC at low shear
stress (0.1–0.2 dynes/cm2) in a pertussis toxin-sensitive
fashion. Quantitatively, Ag-primed T cells displayed 2- to 3-fold
enhanced binding to DC compared with unprimed T cells
(p < 0.01). In contrast to naive T cells, primed T
cell arrest was largely inhibited by pertussis toxin, neutralization of
the CC chemokine, macrophage-derived chemokine (CCL22), or by
desensitization of the CCL22 receptor, CCR4. Our results demonstrate
that DC-derived CCL22 induces rapid binding of activated T cells under
dynamic conditions and that Ag-primed and naive T cells fundamentally
differ with respect to chemokine-dependent binding to
DC.
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