| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of
*
Biochemistry and
Internal Medicine II, Fukushima Medical University School of Medicine, Fukushima, Japan;
Department of Biochemistry, Graduate School of Pharmaceutical Science, Hokkaido University, Sapporo, Japan; and
Department of Applied Biochemistry and Institute of Glycotechnology, Tokai University, Hiratsuka, Japan
Mannose-binding lectin (MBL) is a C-type lectin involved in the
first line of host defense against pathogens and it requires
MBL-associated serine protease (MASP) for activation of the complement
lectin pathway. To elucidate the origin and evolution of MBL, MBL-like
lectin was isolated from the plasma of a urochordate, the solitary
ascidian Halocynthia roretzi, using affinity
chromatography on a yeast mannan-Sepharose. SDS-PAGE of the eluted
proteins revealed a major band of 
36 kDa (p36). p36 cDNA was cloned
from an ascidian hepatopancreas cDNA library. Sequence analysis
revealed that the carboxy-terminal half of the ascidian lectin contains
a carbohydrate recognition domain (CRD) that is homologous to C-type
lectin, but it lacks a collagen-like domain that is present in
mammalian MBLs. Purified p36 binds specifically to glucose but not to
mannose or N-acetylglucosamine, and it was designated
glucose-binding lectin (GBL). The two ascidian MASPs associated with
GBL activate ascidian C3, which had been reported to act as an opsonin.
The removal of GBL-MASPs complex from ascidian plasma using Ab against
GBL inhibits C3-dependent phagocytosis. These observations strongly
suggest that GBL acts as a recognition molecule and that the primitive
complement system, consisting of the lectin-proteases complex and C3,
played a major role in innate immunity before the evolution of an
adaptive immune system in vertebrates.
This article has been cited by other articles:
|
|
 |
|
  S. Huang, S. Yuan, L. Guo, Y. Yu, J. Li, T. Wu, T. Liu, M. Yang, K. Wu, H. Liu, et al. Genomic analysis of the immune gene repertoire of amphioxus reveals extraordinary innate complexity and diversity Genome Res., July 1, 2008; 18(7): 1112 - 1126. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A Bulgakov, M. G Eliseikina, I. Y. Petrova, E. L Nazarenko, S. N Kovalchuk, V. B Kozhemyako, and V. A Rasskazov Molecular and Biological Characterization of a Mannan-Binding Lectin from the Holothurian Apostichopus Japonicus Glycobiology, December 1, 2007; 17(12): 1284 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nakao, T. Kajiya, Y. Sato, T. Somamoto, Y. Kato-Unoki, M. Matsushita, M. Nakata, T. Fujita, and T. Yano Lectin Pathway of Bony Fish Complement: Identification of Two Homologs of the Mannose-Binding Lectin Associated with MASP2 in the Common Carp (Cyprinus carpio) J. Immunol., October 15, 2006; 177(8): 5471 - 5479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. M. Kim, K.-I. Park, K.-S. Choi, R. A. Alvarez, R. D. Cummings, and M. Cho Lectin from the Manila Clam Ruditapes philippinarum Is Induced upon Infection with the Protozoan Parasite Perkinsus olseni J. Biol. Chem., September 15, 2006; 281(37): 26854 - 26864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Takahashi, D. Iwaki, A. Matsushita, M. Nakata, M. Matsushita, Y. Endo, and T. Fujita Cloning and characterization of mannose-binding lectin from lamprey (agnathans). J. Immunol., April 15, 2006; 176(8): 4861 - 4868. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Matsushita, A. Matsushita, Y. Endo, M. Nakata, N. Kojima, T. Mizuochi, and T. Fujita Origin of the classical complement pathway: Lamprey orthologue of mammalian C1q acts as a lectin PNAS, July 6, 2004; 101(27): 10127 - 10131. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Woods, K. E. Roper, M. Gauthier, L. M. Bebell, K. Sung, B. M. Degnan, and M. F. Lavin Gene expression during early ascidian metamorphosis requires signalling by Hemps, an EGF-like protein Development, June 15, 2004; 131(12): 2921 - 2933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Endo, M. Nonaka, H. Saiga, Y. Kakinuma, A. Matsushita, M. Takahashi, M. Matsushita, and T. Fujita Origin of Mannose-Binding Lectin-Associated Serine Protease (MASP)-1 and MASP-3 Involved in the Lectin Complement Pathway Traced Back to the Invertebrate, Amphioxus J. Immunol., May 1, 2003; 170(9): 4701 - 4707. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Quesenberry, H. Ahmed, M. T. Elola, N. O'Leary, and G. R. Vasta Diverse Lectin Repertoires in Tunicates Mediate Broad Recognition and Effector Innate Immune Responses Integr. Comp. Biol., April 1, 2003; 43(2): 323 - 330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Davidson and B. J. Swalla A molecular analysis of ascidian metamorphosis reveals activation of an innate immune response Development, March 12, 2003; 129(20): 4739 - 4751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ambrus, P. Gal, M. Kojima, K. Szilagyi, J. Balczer, J. Antal, L. Graf, A. Laich, B. E. Moffatt, W. Schwaeble, et al. Natural Substrates and Inhibitors of Mannan-Binding Lectin-Associated Serine Protease-1 and -2: A Study on Recombinant Catalytic Fragments J. Immunol., February 1, 2003; 170(3): 1374 - 1382. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
