| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
,
*
Beirne B. Carter Center for Immunology Research and
Department of Pathology and Microbiology, University of Virginia Health Sciences Center, Charlottesville, VA 22908
Beirne B. Carter Center for Immunology Research and
Department of Pathology and Microbiology, University of Virginia Health Sciences Center, Charlottesville, VA 22908
CTL play a major role in the clearance of respiratory syncytial
virus (RSV) during experimental pulmonary infection. The fusion (F)
glycoprotein of RSV is a protective Ag that elicits CTL and Ab response
against RSV infection in BALB/c mice. We used the strategy of screening
a panel of overlapping synthetic peptides corresponding to the RSV F
protein and identified an immunodominant H-2Kd-restricted
epitope (F85–93; KYKNAVTEL) recognized by CD8+
T cells from BALB/c mice. We enumerated the F-specific CD8+
T cell response in the lungs of infected mice by flow cytometry using
tetramer staining and intracellular cytokine synthesis. During primary
infection, F85–93-specific effector CD8+ T
cells constitute 
4.8% of pulmonary CD8+ T cells at the
peak of the primary response (day 8), whereas matrix 2-specific
CD8+ T cells constituted 50% of the responding
CD8+ T cell population in the lungs. When RSV F-immune mice
undergo a challenge RSV infection, the F-specific CD8+ T
cell response is accelerated and dominates, whereas the primary
response to the matrix 2 epitope in the lungs is reduced by 20-fold.
In addition, we found that activated F-specific effector
CD8+ T cells isolated from the lungs of RSV-infected mice
exhibited a lower than expected frequency of IFN-
-producing
CD8+ T cells and were significantly impaired in ex vivo
cytolytic activity compared with competent F-specific effector
CD8+ T cells generated in vitro. The significance of these
results for the regulation of the CD8+ T cell response to
RSV is discussed.
This article has been cited by other articles:
|
|
 |
|
  M. L. Moore, M. H. Chi, C. Luongo, N. W. Lukacs, V. V. Polosukhin, M. M. Huckabee, D. C. Newcomb, U. J. Buchholz, J. E. Crowe Jr., K. Goleniewska, et al. A Chimeric A2 Strain of Respiratory Syncytial Virus (RSV) with the Fusion Protein of RSV Strain Line 19 Exhibits Enhanced Viral Load, Mucus, and Airway Dysfunction J. Virol., May 1, 2009; 83(9): 4185 - 4194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Ruckwardt, K. L. Bonaparte, M. C. Nason, and B. S. Graham Regulatory T Cells Promote Early Influx of CD8+ T Cells in the Lungs of Respiratory Syncytial Virus-Infected Mice and Diminish Immunodominance Disparities J. Virol., April 1, 2009; 83(7): 3019 - 3028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Zhang and R. A. Tripp RNA Interference Inhibits Respiratory Syncytial Virus Replication and Disease Pathogenesis without Inhibiting Priming of the Memory Immune Response J. Virol., December 15, 2008; 82(24): 12221 - 12231. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Olson, S. M. Hartwig, and S. M. Varga The Number of Respiratory Syncytial Virus (RSV)-Specific Memory CD8 T Cells in the Lung Is Critical for Their Ability to Inhibit RSV Vaccine-Enhanced Pulmonary Eosinophilia J. Immunol., December 1, 2008; 181(11): 7958 - 7968. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Johnstone, S. Guil, M. A. Rico, B. Garcia-Barreno, D. Lopez, J. A. Melero, and M. Del Val Relevance of viral context and diversity of antigen-processing routes for respiratory syncytial virus cytotoxic T-lymphocyte epitopes J. Gen. Virol., September 1, 2008; 89(9): 2194 - 2203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. B. Fulton, M. R. Olson, and S. M. Varga Regulation of Cytokine Production by Virus-Specific CD8 T Cells in the Lungs J. Virol., August 15, 2008; 82(16): 7799 - 7811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Castilow, M. R. Olson, D. K. Meyerholz, and S. M. Varga Differential Role of Gamma Interferon in Inhibiting Pulmonary Eosinophilia and Exacerbating Systemic Disease in Fusion Protein-Immunized Mice Undergoing Challenge Infection with Respiratory Syncytial Virus J. Virol., March 1, 2008; 82(5): 2196 - 2207. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Arimilli, E. M. Palmer, and M. A. Alexander-Miller Loss of function in virus-specific lung effector T cells is independent of infection J. Leukoc. Biol., March 1, 2008; 83(3): 564 - 574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Vallbracht, B. Jessen, S. Mrusek, A. Enders, P. L. Collins, S. Ehl, and C. D. Krempl Influence of a Single Viral Epitope on T Cell Response and Disease After Infection of Mice with Respiratory Syncytial Virus J. Immunol., December 15, 2007; 179(12): 8264 - 8273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Melendi, F. Zavala, U. J. Buchholz, G. Boivin, P. L. Collins, S. R. Kleeberger, and F. P. Polack Mapping and Characterization of the Primary and Anamnestic H-2d-Restricted Cytotoxic T-Lymphocyte Response in Mice against Human Metapneumovirus J. Virol., October 15, 2007; 81(20): 11461 - 11467. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Olson and S. M. Varga CD8 T Cells Inhibit Respiratory Syncytial Virus (RSV) Vaccine-Enhanced Disease J. Immunol., October 15, 2007; 179(8): 5415 - 5424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Lee, S. A. Miller, D. W. Wright, M. T. Rock, and J. E. Crowe Jr. Tissue-Specific Regulation of CD8+ T-Lymphocyte Immunodominance in Respiratory Syncytial Virus Infection J. Virol., March 1, 2007; 81(5): 2349 - 2358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Moore, M. H. Chi, W. Zhou, K. Goleniewska, J. F. O'Neal, J. N. Higginbotham, and R. S. Peebles Jr. Cutting Edge: Oseltamivir Decreases T Cell GM1 Expression and Inhibits Clearance of Respiratory Syncytial Virus: Potential Role of Endogenous Sialidase in Antiviral Immunity J. Immunol., March 1, 2007; 178(5): 2651 - 2654. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bukreyev, M. E. Serra, F. R. Laham, G. A. Melendi, S. R. Kleeberger, P. L. Collins, and F. P. Polack The Cysteine-Rich Region and Secreted Form of the Attachment G Glycoprotein of Respiratory Syncytial Virus Enhance the Cytotoxic T-Lymphocyte Response despite Lacking Major Histocompatibility Complex Class I-Restricted Epitopes. J. Virol., June 1, 2006; 80(12): 5854 - 5861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Martinez-Sobrido, N. Gitiban, A. Fernandez-Sesma, J. Cros, S. E. Mertz, N. A. Jewell, S. Hammond, E. Flano, R. K. Durbin, A. Garcia-Sastre, et al. Protection against Respiratory Syncytial Virus by a Recombinant Newcastle Disease Virus Vector J. Virol., February 1, 2006; 80(3): 1130 - 1139. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. W. Claassen, P. A. A. van der Kant, Z. S. Rychnavska, G. M. van Bleek, A. J. Easton, and R. G. van der Most Activation and Inactivation of Antiviral CD8 T Cell Responses during Murine Pneumovirus Infection J. Immunol., November 15, 2005; 175(10): 6597 - 6604. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Braciale Respiratory Syncytial Virus and T Cells: Interplay between the Virus and the Host Adaptive Immune System Proceedings of the ATS, August 1, 2005; 2(2): 141 - 146. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. P. Polack, P. M. Irusta, S. J. Hoffman, M. P. Schiatti, G. A. Melendi, M. F. Delgado, F. R. Laham, B. Thumar, R. M. Hendry, J. A. Melero, et al. The cysteine-rich region of respiratory syncytial virus attachment protein inhibits innate immunity elicited by the virus and endotoxin PNAS, June 21, 2005; 102(25): 8996 - 9001. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Johnson, S. E. Mertz, N. Gitiban, S. Hammond, R. LeGallo, R. K. Durbin, and J. E. Durbin Role for Innate IFNs in Determining Respiratory Syncytial Virus Immunopathology J. Immunol., June 1, 2005; 174(11): 7234 - 7241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. W. Lawrence, R. M. Ream, and T. J. Braciale Frequency, Specificity, and Sites of Expansion of CD8+ T Cells during Primary Pulmonary Influenza Virus Infection J. Immunol., May 1, 2005; 174(9): 5332 - 5340. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Johnstone, P. de Leon, F. Medina, J. A. Melero, B. Garcia-Barreno, and M. D. Val Shifting immunodominance pattern of two cytotoxic T-lymphocyte epitopes in the F glycoprotein of the Long strain of respiratory syncytial virus J. Gen. Virol., November 1, 2004; 85(11): 3229 - 3238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. de Waal, S. Yuksel, A. H. Brandenburg, J. P. M. Langedijk, K. Sintnicolaas, G. M. G. M. Verjans, A. D. M. E. Osterhaus, and R. L. de Swart Identification of a Common HLA-DP4-Restricted T-Cell Epitope in the Conserved Region of the Respiratory Syncytial Virus G Protein J. Virol., February 15, 2004; 78(4): 1775 - 1781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Chang, S. Y. Choi, H. T. Jin, Y. C. Sung, and T. J. Braciale Improved Effector Activity and Memory CD8 T Cell Development by IL-2 Expression during Experimental Respiratory Syncytial Virus Infection J. Immunol., January 1, 2004; 172(1): 503 - 508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Gray, G. D. Parks, and M. A. Alexander-Miller High Avidity CD8+ T Cells Are the Initial Population Elicited Following Viral Infection of the Respiratory Tract J. Immunol., January 1, 2003; 170(1): 174 - 181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. van Bleek, M. C. Poelen, R. van der Most, H. F. Brugghe, H. A. M. Timmermans, C. J. Boog, P. Hoogerhout, H. G. Otten, and C. A. C. M. van Els Identification of Immunodominant Epitopes Derived from the Respiratory Syncytial Virus Fusion Protein That Are Recognized by Human CD4 T Cells J. Virol., December 20, 2002; 77(2): 980 - 988. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Chen, K.-F. Hu, B. Rozell, C. Orvell, B. Morein, and P. Liljestrom Vaccination with Recombinant Alphavirus or Immune-Stimulating Complex Antigen Against Respiratory Syncytial Virus J. Immunol., September 15, 2002; 169(6): 3208 - 3216. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. F. Greten and J. P. Schneck Development and Use of Multimeric Major Histocompatibility Complex Molecules Clin. Vaccine Immunol., March 1, 2002; 9(2): 216 - 220. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
