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Conventional and Surrogate Light Chains Differentially Regulate Ig µ and Dµ Heavy Chain Maturation and Surface Expression¹

Department of Microbiology and Immunology and Morse Institute for Molecular Genetics, State University of New York-Downstate Medical Center, Brooklyn, NY 11203

Positive selection of precursor (pre-) B cells by Ig membrane µ H chains (µm HC) and counterselection mediated by the truncated HC Dµ depend on the ability of each HC to form a pre-B cell receptor (pre-BCR) signaling complex with the surrogate L chain (SLC) components 5 and Vpre-B. To better understand how pre-BCR signaling output is determined by its Ig components and the SLC, we investigated the regulation of pre-BCR surface expression and HC secretory maturation in a new nonlymphoid system. We took this approach as a means to distinguish B-lineage-specific effects from pre-BCR-intrinsic properties that may influence these aspects of pre-BCR homeostasis necessary for signaling. As in pre-B cells, the SLC in nonlymphoid cells supported only a limited degree of µm HC maturation and low pre-BCR surface expression levels compared with conventional LCs, indicating that this was due to an intrinsic property of the SLC. We identified the non-Ig region of 5 as harboring the restrictive activity responsible for this phenotype. This property of 5 was also evident with Dµ, but the overall SLC- and L chain-dependent requirements for Dµ maturation and surface expression were markedly different from those for µm. Surprisingly, Dµ was modified in an unusual manner that was only dependent on Vpre-B. These results establish a novel function of 5 in limiting surface pre-BCR levels and reveal biochemical properties of Ig molecules that may underlie the diverse consequences of pre-BCR signaling in vivo by different HCs.

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