| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
-Specific CD8+ CTLs by TCR V-Derived Peptides Bound to HLA-E1
Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032
Previous studies have identified murine and human regulatory
CD8+ T cells specific for TCR-V
families expressed on
autologous activated CD4+ T cells. In the mouse, these
regulatory CD8+ T cells were shown to be restricted by the
MHC class Ib molecule, Qa-1. In the present study, we asked whether
HLA-E, the human functional equivalent of Qa-1, binds V peptides and
whether the HLA-E/V-peptide complex induces and restricts human
CD8+ CTLs. We first created stable HLA-E gene transfectants
of the C1R cell line (C1R-E). Two putative HLA-E binding nonapeptides
identified in human TCR V1 and V2 chains (SLELGDSAL and
LLLGPGSGL, respectively) were shown to bind to HLA-E. CD8+
T cells could be primed in vitro by C1R-E cells loaded with the V1
(C1R-E/V1) or V2 (C1R-E/V2) peptide to preferentially kill C1R-E
cells loaded with the respective inducing V peptide, compared with
targets loaded with the other peptides. Priming CD8+ T
cells with untreated C1R-E cells did not induce V-specific CTLs. Of
perhaps more physiological relevance was the finding that the
CD8+ CTLs primed by C1R-E/V1 also preferentially killed
activated autologous TCR V1+. Similar results were
observed in reciprocal experiments using C1R-E/V2 for priming.
Furthermore, anti-CD8 and anti-MHC class I mAbs inhibited this
V-specific killing of C1R-E and CD4+ T cell targets.
Taken together, the data provide evidence that certain TCR-V
peptides can be presented by HLA-E to further induce V-specific
CD8+ CTLs.
This article has been cited by other articles:
|
|
 |
|
  M. Sensi, G. Pietra, A. Molla, G. Nicolini, C. Vegetti, I. Bersani, E. Millo, E. Weiss, L. Moretta, M. C. Mingari, et al. Peptides with dual binding specificity for HLA-A2 and HLA-E are encoded by alternatively spliced isoforms of the antioxidant enzyme peroxiredoxin 5 Int. Immunol., March 1, 2009; 21(3): 257 - 268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Lo Monaco, L. Sibilio, E. Melucci, E. Tremante, M. Suchanek, V. Horejsi, A. Martayan, and P. Giacomini HLA-E: Strong Association with {beta}2-Microglobulin and Surface Expression in the Absence of HLA Class I Signal Sequence-Derived Peptides J. Immunol., October 15, 2008; 181(8): 5442 - 5450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Chen, L. Zhang, B. Liang, Y. Saenger, J. Li, L. Chess, and H. Jiang Perceiving the avidity of T cell activation can be translated into peripheral T cell regulation PNAS, December 18, 2007; 104(51): 20472 - 20477. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Coupel, A. Moreau, M. Hamidou, V. Horejsi, J.-P. Soulillou, and B. Charreau Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation Blood, April 1, 2007; 109(7): 2806 - 2814. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Uss, A. T. Rowshani, B. Hooibrink, N. M. Lardy, R. A. W. van Lier, and I. J. M. ten Berge CD103 Is a Marker for Alloantigen-Induced Regulatory CD8+ T Cells. J. Immunol., September 1, 2006; 177(5): 2775 - 2783. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Derre, M. Corvaisier, B. Charreau, A. Moreau, E. Godefroy, A. Moreau-Aubry, F. Jotereau, and N. Gervois Expression and Release of HLA-E by Melanoma Cells and Melanocytes: Potential Impact on the Response of Cytotoxic Effector Cells. J. Immunol., September 1, 2006; 177(5): 3100 - 3107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. K. Tennakoon, R. S. Mehta, S. B. Ortega, V. Bhoj, M. K. Racke, and N. J. Karandikar Therapeutic Induction of Regulatory, Cytotoxic CD8+ T Cells in Multiple Sclerosis. J. Immunol., June 1, 2006; 176(11): 7119 - 7129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Jiang and L. Chess Regulation of immune responses by T cells. N. Engl. J. Med., March 16, 2006; 354(11): 1166 - 1176. [Full Text] [PDF]
|
|
|
|
 |
|
 J. Nattermann, H. D. Nischalke, V. Hofmeister, G. Ahlenstiel, H. Zimmermann, L. Leifeld, E. H. Weiss, T. Sauerbruch, and U. Spengler The HLA-A2 Restricted T Cell Epitope HCV Core35-44 Stabilizes HLA-E Expression and Inhibits Cytolysis Mediated by Natural Killer Cells Am. J. Pathol., February 1, 2005; 166(2): 443 - 453. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Pietra, C. Romagnani, P. Mazzarino, M. Falco, E. Millo, A. Moretta, L. Moretta, and M. C. Mingari HLA-E-restricted recognition of cytomegalovirus-derived peptides by human CD8+ cytolytic T lymphocytes PNAS, September 16, 2003; 100(19): 10896 - 10901. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. A. Bland, M. K. Lemberg, A. J. McMichael, B. Martoglio, and V. M. Braud Requirement of the Proteasome for the Trimming of Signal Peptide-derived Epitopes Presented by the Nonclassical Major Histocompatibility Complex Class I Molecule HLA-E J. Biol. Chem., September 5, 2003; 278(36): 33747 - 33752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Miller, D. A. Weber, C. Ibegbu, J. Pohl, J. D. Altman, and P. E. Jensen Analysis of HLA-E Peptide-Binding Specificity and Contact Residues in Bound Peptide Required for Recognition by CD94/NKG2 J. Immunol., August 1, 2003; 171(3): 1369 - 1375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Strong, M. A. Holmes, P. Li, L. Braun, N. Lee, and D. E. Geraghty HLA-E Allelic Variants. CORRELATING DIFFERENTIAL EXPRESSION, PEPTIDE AFFINITIES, CRYSTAL STRUCTURES, AND THERMAL STABILITIES J. Biol. Chem., February 7, 2003; 278(7): 5082 - 5090. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Romagnani, G. Pietra, M. Falco, E. Millo, P. Mazzarino, R. Biassoni, A. Moretta, L. Moretta, and M. C. Mingari Identification of HLA-E-specific alloreactive T lymphocytes: A cell subset that undergoes preferential expansion in mixed lymphocyte culture and displays a broad cytolytic activity against allogeneic cells PNAS, August 20, 2002; 99(17): 11328 - 11333. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
