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5-Deficient Mice1
,
,
,||
*
Division of Developmental and Clinical Immunology, and Departments of
Medicine,
Pediatrics,
Microbiology, and
¶ Pathology, University of Alabama, Birmingham, AL 35294;
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The Howard Hughes Medical Institute, Birmingham, AL 35294; and
#
Department of Immune Regulation, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan
The VpreB/
5 surrogate L chain complex is an essential component
of the pre-B cell receptor, the expression of which serves as an
important checkpoint in B cell development. Surrogate L chains also may
serve as components of murine pro-B cell receptors whose function is
unknown. We have produced two new mAbs, R3 and R5, that recognize a
different VpreB epitope than the one recognized by the previously
described VP245 anti-mouse VpreB Ab. These Abs were used to confirm
the expression of surrogate L chains on wild-type pro-B and pre-B cell
lines. Although undetectable on the cell surface, VpreB was found to be
normally expressed within B lineage cells of 5-deficient mice.
Nevertheless, VpreB expression was extinguished at the B cell stage of
differentiation in these mice. The normal pattern of VpreB expression
in 5-deficient mice excludes an essential role for pro-B and pre-B
cell receptors in VpreB
regulation.
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