The JI Acurri Cytometers
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schmeissner, P. J.
Right arrow Articles by Marcantonio, E. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schmeissner, P. J.
Right arrow Articles by Marcantonio, E. E.
The Journal of Immunology, 2001, 167: 3715-3724.
Copyright © 2001 by The American Association of Immunologists

Integrin Functions Play a Key Role in the Differentiation of Thymocytes In Vivo1

Peter J. Schmeissner*, Haichun Xie*, Lubomir B. Smilenov*, Fengyu Shu{dagger} and Eugene E. Marcantonio2,*

* Departments of Pathology and Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032; and {dagger} Department of Microbiology, University of Alabama, Birmingham, AL 35294

T cells express a variety of surface proteins as they develop to maturity in the thymus. In addition to the TCR-CD3 complex and the two major coreceptors, CD4 and CD8, other surface proteins expressed include receptors for cytokines, growth factors, counterreceptors, and extracellular matrix molecules. To determine the role of integrin adhesion receptors in T cell development, we have expressed a trans-dominant inhibitor of integrin function in the thymus. This inhibitor leads to a block of adhesion to fibronectin due to reduced activation of integrin receptors. This reduced adhesion leads to a partial block in differentiation from CD4-CD8- cells to CD4+CD8+ cells, after the CD25+ stage, suggesting that integrins are important during Lck-mediated differentiation. Furthermore, the overall production of CD4+ cells is reduced compared with that of CD8+ cells without changes in negative selection, suggesting that integrins may be involved in the determination of the fate of the cell as well. These results demonstrate that integrin receptor function is required for proper thymocyte development in vivo.




This article has been cited by other articles:


Home page
 FASEB J.Home page
Y. Gong, R. Zhang, J. Zhang, L. Xu, F. Zhang, W. Xu, Y. Wang, Y. Chu, and S. Xiong
{alpha}-Dystroglycan is involved in positive selection of thymocytes by participating in immunological synapse formation
FASEB J, May 1, 2008; 22(5): 1426 - 1439.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. N. Wu, S. Gheith, N. A. Bezman, Q.-H. Liu, L. V. Fostel, A. M. Swanson, B. D. Freedman, G. A. Koretzky, and E. J. Peterson
Adhesion- and Degranulation-Promoting Adapter Protein Is Required for Efficient Thymocyte Development and Selection.
J. Immunol., June 1, 2006; 176(11): 6681 - 6689.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. L. Scimone, I. Aifantis, I. Apostolou, H. von Boehmer, and U. H. von Andrian
A multistep adhesion cascade for lymphoid progenitor cell homing to the thymus
PNAS, May 2, 2006; 103(18): 7006 - 7011.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
F. Vianello, P. Kraft, Y. T. Mok, W. K. Hart, N. White, and M. C. Poznansky
A CXCR4-Dependent Chemorepellent Signal Contributes to the Emigration of Mature Single-Positive CD4 Cells from the Fetal Thymus
J. Immunol., October 15, 2005; 175(8): 5115 - 5125.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. Lombardi, D. Burzyn, J. Mundinano, P. Berguer, P. Bekinschtein, H. Costa, L. F. Castillo, A. Goldman, R. Meiss, I. Piazzon, et al.
Cathepsin-L Influences the Expression of Extracellular Matrix in Lymphoid Organs and Plays a Role in the Regulation of Thymic Output and of Peripheral T Cell Number
J. Immunol., June 1, 2005; 174(11): 7022 - 7032.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. A. Calderwood, V. Tai, G. Di Paolo, P. De Camilli, and M. H. Ginsberg
Competition for Talin Results in Trans-dominant Inhibition of Integrin Activation
J. Biol. Chem., July 9, 2004; 279(28): 28889 - 28895.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Wu, J. R. Rodgers, X.-Y. D. Perrard, J. L. Perrard, J. E. Prince, Y. Abe, B. K. Davis, G. Dietsch, C. W. Smith, and C. M. Ballantyne
Deficiency of CD11b or CD11d Results in Reduced Staphylococcal Enterotoxin-Induced T Cell Response and T Cell Phenotypic Changes
J. Immunol., July 1, 2004; 173(1): 297 - 306.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Laakko and R. L. Juliano
Adhesion Regulation of Stromal Cell-derived Factor-1 Activation of ERK in Lymphocytes by Phosphatases
J. Biol. Chem., August 22, 2003; 278(34): 31621 - 31628.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. Niu, H. Xie, and E. E. Marcantonio
Integrin-mediated Tyrosine Phosphorylation of Shc in T Cells Is Regulated by Protein Kinase C-dependent Phosphorylations of Lck
Mol. Biol. Cell, February 1, 2003; 14(2): 349 - 360.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Kutlesa, J. T. Wessels, A. Speiser, I. Steiert, C. A. Muller, and G. Klein
E-cadherin-mediated interactions of thymic epithelial cells with CD103+ thymocytes lead to enhanced thymocyte cell proliferation
J. Cell Sci., January 12, 2002; 115(23): 4505 - 4515.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.