The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kawashima, T.
Right arrow Articles by Kitamura, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kawashima, T.
Right arrow Articles by Kitamura, T.
Right arrowPubmed/NCBI databases
*Substance via MeSH
Medline Plus Health Information
*Dietary Proteins
The Journal of Immunology, 2001, 167: 3652-3660.
Copyright © 2001 by The American Association of Immunologists

STAT5 Induces Macrophage Differentiation of M1 Leukemia Cells Through Activation of IL-6 Production Mediated by NF-{kappa}B p651

Toshiyuki Kawashima*,§, Ken Murata*, Shizuo Akira{ddagger}, Yukio Tonozuka*, Yukinori Minoshima*, Sizhou Feng*, Hidetoshi Kumagai*, Hiromichi Tsuruga*, Yasuo Ikeda§, Shigetaka Asano{dagger}, Tetsuya Nosaka* and Toshio Kitamura2,*

* Division of Hematopoietic Factors and {dagger} Department of Hematology/Oncology, Institute of Medical Science, University of Tokyo, Tokyo, Japan; {ddagger} Department of Host Defense, Core Research for Engineering, Science, and Technology of Japan Science and Technology Corporation, Research Institute for Microbial Diseases, Osaka University, Suita, Japan; and § Division of Hematology, Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan

We recently demonstrated that STAT5 can induce a variety of biological functions in mouse IL-3-dependent Ba/F3 cells; STAT5-induced expression of pim-1, p21WAF/Cip1, and suppressor of cytokine signaling-1/STAT-induced STAT inhibitor-1/Janus kinase binding protein is responsible for induction of proliferation, differentiation, and apoptosis, respectively. In the present study, using a constitutively active STAT5A (STAT5A1*6), we show that STAT5 induces macrophage differentiation of mouse leukemic M1 cells through a distinct mechanism, autocrine production of IL-6. The supernatant of STAT5A1*6-transduced cells contained sufficient concentrations of IL-6 to induce macrophage differentiation of parental M1 cells, and STAT3 was phosphorylated on their tyrosine residues in these cells. Treatment of the cells with anti-IL-6 blocking Abs profoundly inhibited the differentiation. We also found that the STAT5A1*6 transactivated the IL-6 promoter, which was mediated by the enhanced binding of NF-{kappa}B p65 (RelA) to the promoter region of IL-6. These findings indicate that STAT5A cooperates with Rel/NF-{kappa}B to induce production of IL-6, thereby inducing macrophage differentiation of M1 cells in an autocrine manner. In summary, we have shown a novel mechanism by which STAT5 induces its pleiotropic functions. Cytokines




This article has been cited by other articles:


Home page
 J. Immunol.Home page
E. Kuroda, V. Ho, J. Ruschmann, F. Antignano, M. Hamilton, M. J. Rauh, A. Antov, R. A. Flavell, L. M. Sly, and G. Krystal
SHIP Represses the Generation of IL-3-Induced M2 Macrophages by Inhibiting IL-4 Production from Basophils
J. Immunol., September 15, 2009; 183(6): 3652 - 3660.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
T. Kawashima, Y. C. Bao, Y. Nomura, Y. Moon, Y. Tonozuka, Y. Minoshima, T. Hatori, A. Tsuchiya, M. Kiyono, T. Nosaka, et al.
Rac1 and a GTPase-activating protein, MgcRacGAP, are required for nuclear translocation of STAT transcription factors
J. Cell Biol., December 18, 2006; 175(6): 937 - 946.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
Y. Kato, A. Iwama, Y. Tadokoro, K. Shimoda, M. Minoguchi, S. Akira, M. Tanaka, A. Miyajima, T. Kitamura, and H. Nakauchi
Selective activation of STAT5 unveils its role in stem cell self-renewal in normal and leukemic hematopoiesis
J. Exp. Med., July 5, 2005; 202(1): 169 - 179.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K.-T. Kim, K. Baird, J.-Y. Ahn, P. Meltzer, M. Lilly, M. Levis, and D. Small
Pim-1 is up-regulated by constitutively activated FLT3 and plays a role in FLT3-mediated cell survival
Blood, February 15, 2005; 105(4): 1759 - 1767.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
H. Nakajima, M. Takenaka, J.-Y. Kaimori, T. Hamano, H. Iwatani, T. Sugaya, T. Ito, M. Hori, and E. Imai
Activation of the Signal Transducer and Activator of Transcription Signaling Pathway in Renal Proximal Tubular Cells by Albumin
J. Am. Soc. Nephrol., February 1, 2004; 15(2): 276 - 285.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. J. Lyon, R. E. Law, and W. A. Hsueh
Minireview: Adiposity, Inflammation, and Atherogenesis
Endocrinology, June 1, 2003; 144(6): 2195 - 2200.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Ghilardi, J. Li, J.-A. Hongo, S. Yi, A. Gurney, and F. J. de Sauvage
A Novel Type I Cytokine Receptor Is Expressed on Monocytes, Signals Proliferation, and Activates STAT-3 and STAT-5
J. Biol. Chem., May 3, 2002; 277(19): 16831 - 16836.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Nakamura, R. Ouchida, T. Kodama, T. Kawashima, Y. Makino, N. Yoshikawa, S. Watanabe, C. Morimoto, T. Kitamura, and H. Tanaka
Cytokine Receptor Common beta Subunit-mediated STAT5 Activation Confers NF-kappa B Activation in Murine proB Cell Line Ba/F3 Cells
J. Biol. Chem., February 15, 2002; 277(8): 6254 - 6265.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.