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. Bohnhorst2,*
*
Institute of Immunology and
Centre for Rheumatic Disease, Rikshospitalet, University Hospital, University of Oslo, Oslo, Norway
Analyses of B cells in the bone marrow and secondary lymphoid
tissues have revealed a broad range of cell surface markers defining B
cell subpopulations, but only a few of these have been used to analyze
B cell subpopulations in peripheral blood (PB). We report here the
delineation of circulating PB B cell subpopulations by staining for
CD19, CD38, and IgD in combination with CD10, CD44, CD77, CD95, CD23,
IgM, and the B cell memory marker CD27. The utility of this approach is
shown by the demonstration of disturbances of circulating B cell
subpopulations in patients with autoimmune disease. Five mature B cell
(Bm) subpopulations were identified in normal PB that were
comparable with the tonsillar Bm1, Bm2, early Bm5, Bm5 subpopulations
and, surprisingly, to the germinal center (GC) founder cell
subpopulation (Bm2' and Bm3
–4), suggesting that some GC
founder cells are circulating. No PB B cells resembled the Bm3 and Bm4
GC cells. Remarkably, some cells with the
CD38-IgD+ phenotype, previously known as naive
Bm1 cells, expressed CD27. The CD38-IgD+
subpopulation therefore includes both naive Bm1 cells and
IgD+ memory B cells. This new classification of B cell
developmental stages reveals disturbances in the proportions of B cell
subpopulations in primary Sjögren’s syndrome (pSS) patients
compared with healthy donors and rheumatoid arthritis patients.
Patients with pSS contained a significantly higher percentage of B
cells in two activated stages, which might reflect a disturbance in B
cell trafficking and/or alteration in B cell differentiation. These
findings could be of diagnostic significance for
pSS.
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