| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
and 
2b Promoters in Murine B Lymphocytes: Evidence for Specific Promoter-Enhancer Interactions1
*
Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden; and
Institute of Immunology, Vilnius, Lithuania
During an immune response, activated B cells develop into high rate
Ig-secreting plasma cells. They also switch from production of IgM to
IgG, IgA, or IgE. This process requires a DNA recombination event,
which is regulated at the transcriptional level by the production of
isotype-specific, sterile germline (GL) transcripts. Induction of these
transcripts is controlled by GL promoters and, possibly, by IgH 3'
enhancers. We investigated the interaction of the GL 
and 
2b
promoters with the HS1,2 enhancer using transiently transfected mouse
primary B cells and cell lines. The constructs used for the
transfections contained a GL promoter upstream and HS1,2 downstream of
a luciferase reporter gene. Both GL and 2b promoters synergized
strongly with the HS1,2 enhancer in activated primary B cells, a mature
B cell line, and a plasma cell line. We show that the major activity of
HS1,2 in activated primary B cells occurs within a 310-bp fragment that
includes NF-
B, OCT, and NF of activated B cells (Ets/AP-1) sites. By
mutating the consensus sequences for various transcription factors, we
have determined which sites in HS1,2 are important for synergy with the
GL and 2b promoters. Our findings indicate that different sites
in HS1,2 might selectively interact with the GL and 2b
promoters. We also provide evidence that B cell-specific activator
protein is not an absolute suppressor of HS1,2
activity.
This article has been cited by other articles:
|
|
 |
|
  J. Laurencikiene, V. Tamosiunas, and E. Severinson Regulation of {epsilon} germline transcription and switch region mutations by IgH locus 3' enhancers in transgenic mice Blood, January 1, 2007; 109(1): 159 - 167. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Volgina, P.-C. Yam, and K. L. Knight A negative regulatory element in the rabbit 3'IgH chromosomal region Int. Immunol., August 1, 2005; 17(8): 973 - 982. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Lin, S. L. Brody, and S. L. Peng Restraint of B Cell Activation by Foxj1-Mediated Antagonism of NF-{kappa}B and IL-6 J. Immunol., July 15, 2005; 175(2): 951 - 958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Reynaud, L. Delpy, L. Fleury, H.-L. Dougier, C. Sirac, and M. Cogne Interallelic Class Switch Recombination Contributes Significantly to Class Switching in Mouse B Cells J. Immunol., May 15, 2005; 174(10): 6176 - 6183. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. A. Dunnick, J. Shi, K. A. Graves, and J. T. Collins The 3' end of the heavy chain constant region locus enhances germline transcription and switch recombination of the four {gamma} genes J. Exp. Med., May 2, 2005; 201(9): 1459 - 1466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ushmorov, O. Ritz, M. Hummel, F. Leithauser, P. Moller, H. Stein, and T. Wirth Epigenetic silencing of the immunoglobulin heavy-chain gene in classical Hodgkin lymphoma-derived cell lines contributes to the loss of immunoglobulin expression Blood, November 15, 2004; 104(10): 3326 - 3334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Sepulveda, A. V. Emelyanov, and B. K. Birshtein NF-{kappa}B and Oct-2 Synergize to Activate the Human 3' Igh hs4 Enhancer in B Cells J. Immunol., January 15, 2004; 172(2): 1054 - 1064. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Heckman, T. Cao, L. Somsouk, H. Duan, J. W. Mehew, C.-y. Zhang, and L. M. Boxer Critical Elements of the Immunoglobulin Heavy Chain Gene Enhancers for Deregulated Expression of Bcl-2 Cancer Res., October 15, 2003; 63(20): 6666 - 6673. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Zhang Accessibility control and machinery of immunoglobulin class switch recombination J. Leukoc. Biol., March 1, 2003; 73(3): 323 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Bae, V. C. Calhoun, M. Levine, E. B. Lewis, and R. A. Drewell Characterization of the intergenic RNA profile at abdominal-A and Abdominal-B in the Drosophila bithorax complex PNAS, December 24, 2002; 99(26): 16847 - 16852. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Peng, S. J. Szabo, and L. H. Glimcher T-bet regulates IgG class switching and pathogenic autoantibody production PNAS, April 16, 2002; 99(8): 5545 - 5550. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
