HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Béchard, D. Articles by Lassalle, P. Search for Related Content PubMed PubMed Citation Articles by Béchard, D. Articles by Lassalle, P. Pubmed/NCBI databases Gene GEO Profiles HomoloGene Protein UniGene Compound via MeSH Substance via MeSH Hazardous Substances DB 12-O-TETRADECANOYLPHORBOL-13-ACETATE

Human Endothelial-Cell Specific Molecule-1 Binds Directly to the Integrin CD11a/CD18 (LFA-1) and Blocks Binding to Intercellular Adhesion Molecule-1¹

David Béchard^*, Arnaud Scherpereel^*, Hamida Hammad^*, Thibaut Gentina^*, Anne Tsicopoulos^*, Marc Aumercier, Jöel Pestel^*, Jean-Paul Dessaint, André-Bernard Tonnel^* and Philippe Lassalle^2,*

^* Institut National de la Santé et de la Recherche Médicale Unité 416, Institut Pasteur de Lille; Centre National de la Recherche Scientifique Unité Mixte de Recherche 8526, Institut de Biologie de Lille; and Laboratoire d’Immunologie, Centre Hospitalier Régional Universitaire, Lille, France

ICAMs are ligands for LFA-1, a major integrin of mononuclear cells involved in the immune and inflammatory processes. We previously showed that endothelial cell specific molecule-1 (ESM-1) is a proteoglycan secreted by endothelial cells under the control of inflammatory cytokines. Here, we demonstrate that ESM-1 binds directly to LFA-1 onto the cell surface of human blood lymphocytes, monocytes, and Jurkat cells. The binding of ESM-1 was equally dependent on Ca²⁺, Mg²⁺, or Mn²⁺ divalent ions, which are specific, saturable, and sensitive to temperature. An anti-CD11a mAb or PMA induced a transient increase in binding, peaking 5 min after activation. Direct binding of ESM-1 to LFA-1 integrin was demonstrated by specific coimmunoprecipitation by CD11a and CD18 mAbs. A cell-free system using a Biacore biosensor confirmed that ESM-1 and LFA-1 dynamically interacted in real time with high affinity (K_d = 18.7 nM). ESM-1 consistently inhibited the specific binding of soluble ICAM-1 to Jurkat cells in a dose-dependent manner. These results suggest that ESM-1 and ICAM-1 interact with LFA-1 on binding sites very close to but distinct from the I domain of CD11a. Through this mechanism, ESM-1 could be implicated in the regulation of the LFA-1/ICAM-1 pathway and may therefore influence both the recruitment of circulating lymphocytes to inflammatory sites and LFA-1-dependent leukocyte adhesion and activation.

This article has been cited by other articles:

				X. Niu, K. Gupta, J. T. Yang, M. J. Shamblott, and A. Levchenko Physical transfer of membrane and cytoplasmic components as a general mechanism of cell-cell communication J. Cell Sci., March 1, 2009; 122(5): 600 - 610. [Abstract] [Full Text] [PDF]

				J. W. Shin, R. Huggenberger, and M. Detmar Transcriptional profiling of VEGF-A and VEGF-C target genes in lymphatic endothelium reveals endothelial-specific molecule-1 as a novel mediator of lymphangiogenesis Blood, September 15, 2008; 112(6): 2318 - 2326. [Abstract] [Full Text] [PDF]

				B. D. Grigoriu, F. Depontieu, A. Scherpereel, D. Gourcerol, P. Devos, T. Ouatas, J.-J. Lafitte, M.-C. Copin, A.-B. Tonnel, P. Lassalle, et al. Endocan Expression and Relationship with Survival in Human Non-Small Cell Lung Cancer Clin. Cancer Res., August 1, 2006; 12(15): 4575 - 4582. [Abstract] [Full Text] [PDF]

				M. A. V. Landgraf, L. L. Martinez, V. M. F. Rastelli, M. d. C. P. Franco, M. Soto-Suazo, R. d. C. A. Tostes, M. H. C. Carvalho, D. Nigro, and Z. B. Fortes Intrauterine Undernutrition in Rats Interferes with Leukocyte Migration, Decreasing Adhesion Molecule Expression in Leukocytes and Endothelial Cells J. Nutr., June 1, 2005; 135(6): 1480 - 1485. [Abstract] [Full Text] [PDF]

				S. Lancel, S. Tissier, S. Mordon, X. Marechal, F. Depontieu, A. Scherpereel, C. Chopin, and R. Neviere Peroxynitrite decomposition catalysts prevent myocardial dysfunction and inflammation in endotoxemic rats J. Am. Coll. Cardiol., June 16, 2004; 43(12): 2348 - 2358. [Abstract] [Full Text] [PDF]

				A. Scherpereel, T. Gentina, B. Grigoriu, S. Senechal, A. Janin, A. Tsicopoulos, F. Plenat, D. Bechard, A.-B. Tonnel, and P. Lassalle Overexpression of Endocan Induces Tumor Formation Cancer Res., September 15, 2003; 63(18): 6084 - 6089. [Abstract] [Full Text] [PDF]

				D. Bechard, T. Gentina, M. Delehedde, A. Scherpereel, M. Lyon, M. Aumercier, R. Vazeux, C. Richet, P. Degand, B. Jude, et al. Endocan Is a Novel Chondroitin Sulfate/Dermatan Sulfate Proteoglycan That Promotes Hepatocyte Growth Factor/Scatter Factor Mitogenic Activity J. Biol. Chem., December 14, 2001; 276(51): 48341 - 48349. [Abstract] [Full Text] [PDF]