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Identification, Cloning, and Recombinant Expression of Procalin, a Major Triatomine Allergen¹

Christopher D. Paddock^*, James H. McKerrow^2,,, Elizabeth Hansell, K. W. Foreman, Ivy Hsieh and Neal Marshall^¶

^* Viral and Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333; Departments of Pathology and Pharmaceutical Chemistry, University of California, and Department of Veterans Affairs Medical Center, San Francisco, CA 94121; and ^¶ Department of Natural Sciences, Notre Dame de Namur University, Belmont, CA 94002

Among the most frequent anaphylactic reactions to insects are those attributed to reduviid bugs. We report the purification and identification of the major salivary allergen of these insects. This 20-kDa protein (procalin) is a member of the lipocalin family, which includes salivary allergens from other invertebrates and mammals. An expression system capable of producing reagent quantities of recombinant allergen was developed in Saccharomyces cerevisiae. Antisera produced against recombinant protein cross-reacts with ELISA with salivary allergen. Recombinant Ag is also shown to react with sera from an allergic patient but not with control sera. By immunolocalization, the source of the salivary Ag is the salivary gland epithelium and its secretions.

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