| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of Medicine (
*
Gastroenterology and
Developmental and Clinical Immunology),
Microbiology, and
Surgery and
¶ Pathology, University of Alabama, and
||
Veterans Affairs Medical Center, Birmingham, AL 35294;
#
Department of Pathology, George Washington University School of Medicine, Washington, DC 20037; and
**
Department of Pediatrics, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298
The intestinal mucosa normally displays minimal inflammation
despite the close proximity between mucosal macrophages and lumenal
bacteria. Macrophages interact with bacteria and their products through
CD14, a surface receptor involved in the response to LPS, and CD89, the
receptor for IgA (Fc
R). Here we show that resident macrophages
isolated from normal human intestine lack CD14 and CD89. The absence of
CD14 and CD89 was not due to the isolation procedure or mucosal cell
products, but was evident at the transcriptional level, as the
macrophages expressed neither CD14- nor CD89-specific mRNAs, but did
express Toll-like receptor 2 and 4 transcripts. Consistent with their
CD14- phenotype, lamina propria macrophages
displayed markedly reduced LPS-induced cytokine production and
LPS-enhanced phagocytosis. In addition, IgA-enhanced phagocytosis was
sharply reduced in lamina propria macrophages. Thus, the absence of
CD14 and CD89 on resident intestinal macrophages, due to down-regulated
gene transcription, causes down-modulated LPS- and IgA-mediated
functions and probably contributes to the low level of inflammation in
normal human intestinal mucosa.
This article has been cited by other articles:
|
|
 |
|
  R. Shen, H. E. Richter, R. H. Clements, L. Novak, K. Huff, D. Bimczok, S. Sankaran-Walters, S. Dandekar, P. R. Clapham, L. E. Smythies, et al. Macrophages in Vaginal but Not Intestinal Mucosa Are Monocyte-Like and Permissive to Human Immunodeficiency Virus Type 1 Infection J. Virol., April 1, 2009; 83(7): 3258 - 3267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. YOSHIOKA, Y. TANIGUCHI, A. YOSHIDA, K. NAKATA, T. NISHIZAWA, H. INAGAWA, C. KOHCHI, and G.-I. SOMA Intracellular Localization of CD14 Protein in Intestinal Macrophages Anticancer Res, March 1, 2009; 29(3): 865 - 869. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Daly, C. Lefevre, K. Nicholas, E. Deane, and P. Williamson CD14 and TLR4 are expressed early in tammar (Macropus eugenii) neonate development J. Exp. Biol., April 15, 2008; 211(8): 1344 - 1351. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Lewis, J. Caldwell, V. Phan, D. Prescott, A. Nazli, A. Wang, J. D. Soderholm, M. H. Perdue, P. M. Sherman, and D. M. McKay Decreased epithelial barrier function evoked by exposure to metabolic stress and nonpathogenic E. coli is enhanced by TNF-{alpha} Am J Physiol Gastrointest Liver Physiol, March 1, 2008; 294(3): G669 - G678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Matysiak-Budnik, I. C. Moura, M. Arcos-Fajardo, C. Lebreton, S. Menard, C. Candalh, K. Ben-Khalifa, C. Dugave, H. Tamouza, G. van Niel, et al. Secretory IgA mediates retrotranscytosis of intact gliadin peptides via the transferrin receptor in celiac disease J. Exp. Med., January 21, 2008; 205(1): 143 - 154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Maheshwari, L. E. Smythies, X. Wu, L. Novak, R. Clements, D. Eckhoff, A. J. Lazenby, W. J. Britt, and P. D. Smith Cytomegalovirus blocks intestinal stroma-induced down-regulation of macrophage HIV-1 infection. J. Leukoc. Biol., November 1, 2006; 80(5): 1111 - 1117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. E. Smythies, A. Maheshwari, R. Clements, D. Eckhoff, L. Novak, H. L. Vu, L. M. Mosteller-Barnum, M. Sellers, and P. D. Smith Mucosal IL-8 and TGF-{beta} recruit blood monocytes: evidence for cross-talk between the lamina propria stroma and myeloid cells J. Leukoc. Biol., September 1, 2006; 80(3): 492 - 499. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Desnues, M. Ihrig, D. Raoult, and J.-L. Mege Whipple's Disease: a Macrophage Disease Clin. Vaccine Immunol., February 1, 2006; 13(2): 170 - 178. [Full Text] [PDF]
|
|
|
|
 |
|
 C Mueller and A J Macpherson Layers of mutualism with commensal bacteria protect us from intestinal inflammation Gut, February 1, 2006; 55(2): 276 - 284. [Full Text] [PDF]
|
|
|
|
 |
|
 N. Kamada, T. Hisamatsu, S. Okamoto, T. Sato, K. Matsuoka, K. Arai, T. Nakai, A. Hasegawa, N. Inoue, N. Watanabe, et al. Abnormally Differentiated Subsets of Intestinal Macrophage Play a Key Role in Th1-Dominant Chronic Colitis through Excess Production of IL-12 and IL-23 in Response to Bacteria J. Immunol., November 15, 2005; 175(10): 6900 - 6908. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hausmann, F. Bataille, T. Spoettl, K. Schreiter, W. Falk, J. Schoelmerich, H. Herfarth, and G. Rogler Physiological Role of Macrophage Inflammatory Protein-3{alpha} Induction during Maturation of Intestinal Macrophages J. Immunol., August 1, 2005; 175(3): 1389 - 1398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E Cario BACTERIAL INTERACTIONS WITH CELLS OF THE INTESTINAL MUCOSA: TOLL-LIKE RECEPTORS AND NOD2 Gut, August 1, 2005; 54(8): 1182 - 1193. [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Schiffrin, M. E. Yousfi, M. Faure, L. Combaret, A. Donnet, S. Blum, C. Obled, and D. Breuille Milk Casein-Based Diet Containing TGF-{beta} Controls the Inflammatory Reaction in the HLA-B27 Transgenic Rat Model JPEN J Parenter Enteral Nutr, July 1, 2005; 29(4_suppl): S141 - S150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Fort, A. Mozaffarian, A. G. Stover, J. d. S. Correia, D. A. Johnson, R. T. Crane, R. J. Ulevitch, D. H. Persing, H. Bielefeldt-Ohmann, P. Probst, et al. A Synthetic TLR4 Antagonist Has Anti-Inflammatory Effects in Two Murine Models of Inflammatory Bowel Disease J. Immunol., May 15, 2005; 174(10): 6416 - 6423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Abreu, M. Fukata, and M. Arditi TLR Signaling in the Gut in Health and Disease J. Immunol., April 15, 2005; 174(8): 4453 - 4460. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Schenk, A. Bouchon, S. Birrer, M. Colonna, and C. Mueller Macrophages Expressing Triggering Receptor Expressed on Myeloid Cells-1 Are Underrepresented in the Human Intestine J. Immunol., January 1, 2005; 174(1): 517 - 524. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Chang, P. McCluskey, and D. Wakefield Expression of Toll-like Receptor 4 and Its Associated Lipopolysaccharide Receptor Complex by Resident Antigen-Presenting Cells in the Human Uvea Invest. Ophthalmol. Vis. Sci., June 1, 2004; 45(6): 1871 - 1878. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Reljic, C. Crawford, S. Challacombe, and J. Ivanyi Mouse IgA inhibits cell growth by stimulating tumor necrosis factor-{alpha} production and apoptosis of macrophage cell lines Int. Immunol., April 1, 2004; 16(4): 607 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Zughaier, Y.-L. Tzeng, S. M. Zimmer, A. Datta, R. W. Carlson, and D. S. Stephens Neisseria meningitidis Lipooligosaccharide Structure-Dependent Activation of the Macrophage CD14/Toll-Like Receptor 4 Pathway Infect. Immun., January 1, 2004; 72(1): 371 - 380. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. D. Smith, G. Meng, J. F. Salazar-Gonzalez, and G. M. Shaw Macrophage HIV-1 infection and the gastrointestinal tract reservoir J. Leukoc. Biol., November 1, 2003; 74(5): 642 - 649. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Hornef, B. H. Normark, A. Vandewalle, and S. Normark Intracellular Recognition of Lipopolysaccharide by Toll-like Receptor 4 in Intestinal Epithelial Cells J. Exp. Med., October 20, 2003; 198(8): 1225 - 1235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Melmed, L. S. Thomas, N. Lee, S. Y. Tesfay, K. Lukasek, K. S. Michelsen, Y. Zhou, B. Hu, M. Arditi, and M. T. Abreu Human Intestinal Epithelial Cells Are Broadly Unresponsive to Toll-Like Receptor 2-Dependent Bacterial Ligands: Implications for Host-Microbial Interactions in the Gut J. Immunol., February 1, 2003; 170(3): 1406 - 1415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. F. Gomez, T. J. Ochoa, I. Herrera-Insua, L. G. Carlin, and T. G. Cleary Lactoferrin Protects Rabbits from Shigella flexneri-Induced Inflammatory Enteritis Infect. Immun., December 1, 2002; 70(12): 7050 - 7053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Hornef, T. Frisan, A. Vandewalle, S. Normark, and A. Richter-Dahlfors Toll-like Receptor 4 Resides in the Golgi Apparatus and Colocalizes with Internalized Lipopolysaccharide in Intestinal Epithelial Cells J. Exp. Med., February 25, 2002; 195(5): 559 - 570. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
