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koberne*
*
Institut für Medizinische Mikrobiologie und Hygiene, Fakultät für Klinische Medizin Mannheim der Universität Heidelberg, Mannheim, Germany; and
Institut für Virologie, Universität Mainz, Mainz, Germany
Little information exists regarding the presentation of antigenic
peptides in infected tissues. In this study the in vivo presentation of
four different CD8 T cell epitopes of Listeria
monocytogenes was monitored. Peptide presentation was measured
by a new, highly sensitive, ex vivo Ag presentation assay that was
based on the testing of freshly isolated cells from infected spleens
with peptide-specific CD8 T cell lines in an IFN-
-specific ELISPOT
assay. Remarkably, the peptide presentation pattern of splenocytes and
that of macrophages purified from spleens of L.
monocytogenes-infected mice were different from those of in
vitro infected macrophage-like cell lines. The in vivo Ag presentation
pattern of splenocytes also exhibited dynamic changes during the first
48 h of infection. In vivo peptide presentation at later time
points postinfection was biased toward immunodominant CD8 T cell
epitopes, while at an early time point, 6 h postinfection,
subdominant and dominant CD8 T cell epitopes were presented with
similar strength. In summary, our studies show that Ag presentation
during an infection is a highly dynamic process that only can be fully
appreciated by the study of cells infected in their physiological
environment.
This article has been cited by other articles:
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J. Janda, P. Schoneberger, M. Skoberne, M. Messerle, H. Russmann, and G. Geginat Cross-Presentation of Listeria-Derived CD8 T Cell Epitopes Requires Unstable Bacterial Translation Products J. Immunol., November 1, 2004; 173(9): 5644 - 5651. [Abstract] [Full Text] [PDF]
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G. A. Corbin and J. T. Harty Duration of Infection and Antigen Display Have Minimal Influence on the Kinetics of the CD4+ T Cell Response to Listeria monocytogenes Infection J. Immunol., November 1, 2004; 173(9): 5679 - 5687. [Abstract] [Full Text] [PDF]
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L. A. Zenewicz, K. E. Foulds, J. Jiang, X. Fan, and H. Shen Nonsecreted Bacterial Proteins Induce Recall CD8 T Cell Responses But Do Not Serve as Protective Antigens J. Immunol., November 15, 2002; 169(10): 5805 - 5812. [Abstract] [Full Text] [PDF]
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M. Skoberne, S. Schenk, H. Hof, and G. Geginat Cross-Presentation of Listeria monocytogenes-Derived CD4 T Cell Epitopes J. Immunol., August 1, 2002; 169(3): 1410 - 1418. [Abstract] [Full Text] [PDF]
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R. J. DiPaolo and E. R. Unanue Cutting Edge: Chemical Dominance Does Not Relate to Immunodominance: Studies of the CD4+ T Cell Response to a Model Antigen J. Immunol., July 1, 2002; 169(1): 1 - 4. [Abstract] [Full Text] [PDF]
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M. Skoberne and G. Geginat Efficient In Vivo Presentation of Listeria monocytogenes- Derived CD4 and CD8 T Cell Epitopes in the Absence of IFN-{gamma} J. Immunol., February 15, 2002; 168(4): 1854 - 1860. [Abstract] [Full Text] [PDF]
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