The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Forsgren, A.
Right arrow Articles by Riesbeck, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Forsgren, A.
Right arrow Articles by Riesbeck, K.
The Journal of Immunology, 2001, 167: 2112-2120.
Copyright © 2001 by The American Association of Immunologists

Isolation and Characterization of a Novel IgD-Binding Protein from Moraxella catarrhalis1

Arne Forsgren, Marta Brant, Andrea Möllenkvist, Anthony Muyombwe, Håkan Janson, Nicolas Woin and Kristian Riesbeck2

Department of Medical Microbiology, Malmö University Hospital, Lund University, Malmö, Sweden

A novel surface protein of the bacterial species Moraxella catarrhalis that displays a high affinity for IgD (MID) was solubilized in Empigen and isolated by ion exchange chromatography and gel filtration. The apparent molecular mass of monomeric MID was estimated to ~200 kDa by SDS-PAGE. The mid gene was cloned and expressed in Escherichia coli. The complete mid nucleotide gene sequence was determined, and the deduced amino acid sequence consists of 2123 residues. The sequence of MID has no similarity to other Ig-binding proteins and differs from all previously described outer membrane proteins of M. catarrhalis. MID was found to exhibit unique Ig-binding properties. Thus, in ELISA, dot blots, and Western blots, MID bound two purified IgD myeloma proteins, four IgD myeloma sera, and finally one IgD standard serum. No binding of MID was detected to IgG, IgM, IgA, or IgE myeloma proteins. MID also bound to the surface-expressed B cell receptor IgD, but not to other membrane molecules on human PBLs. This novel Ig-binding reagent promises to be of theoretical and practical interest in immunological research.




This article has been cited by other articles:


Home page
 Microbiol. Mol. Biol. Rev.Home page
S. P. W. de Vries, H. J. Bootsma, J. P. Hays, and P. W. M. Hermans
Molecular Aspects of Moraxella catarrhalis Pathogenesis
Microbiol. Mol. Biol. Rev., September 1, 2009; 73(3): 389 - 406.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Hallstrom, A. M. Blom, P. F. Zipfel, and K. Riesbeck
Nontypeable Haemophilus influenzae Protein E Binds Vitronectin and Is Important for Serum Resistance
J. Immunol., August 15, 2009; 183(4): 2593 - 2601.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
E. R. LaFontaine, L. E. Snipes, B. Bullard, A. L. Brauer, S. Sethi, and T. F. Murphy
Identification of Domains of the Hag/MID Surface Protein Recognized by Systemic and Mucosal Antibodies in Adults with Chronic Obstructive Pulmonary Disease following Clearance of Moraxella catarrhalis
Clin. Vaccine Immunol., May 1, 2009; 16(5): 653 - 659.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Jendholm, M. Morgelin, M. L. A. Perez Vidakovics, M. Carlsson, H. Leffler, L.-O. Cardell, and K. Riesbeck
Superantigen- and TLR-Dependent Activation of Tonsillar B Cells after Receptor-Mediated Endocytosis
J. Immunol., April 15, 2009; 182(8): 4713 - 4720.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
J. Jendholm, M. Samuelsson, L.-O. Cardell, A. Forsgren, and K. Riesbeck
Moraxella catarrhalis-dependent tonsillar B cell activation does not lead to apoptosis but to vigorous proliferation resulting in nonspecific IgM production
J. Leukoc. Biol., June 1, 2008; 83(6): 1370 - 1378.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
T. C. Hoopman, W. Wang, C. A. Brautigam, J. L. Sedillo, T. J. Reilly, and E. J. Hansen
Moraxella catarrhalis Synthesizes an Autotransporter That Is an Acid Phosphatase
J. Bacteriol., February 15, 2008; 190(4): 1459 - 1472.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
R. Balder, J. Hassel, S. Lipski, and E. R. Lafontaine
Moraxella catarrhalis Strain O35E Expresses Two Filamentous Hemagglutinin-Like Proteins That Mediate Adherence to Human Epithelial Cells
Infect. Immun., June 1, 2007; 75(6): 2765 - 2775.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Samuelsson, T. Hallstrom, A. Forsgren, and K. Riesbeck
Characterization of the IgD Binding Site of Encapsulated Haemophilus influenzae Serotype b
J. Immunol., May 15, 2007; 178(10): 6316 - 6319.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
W. Wang, M. M. Pearson, A. S. Attia, R. J. Blick, and E. J. Hansen
A UspA2H-Negative Variant of Moraxella catarrhalis Strain O46E Has a Deletion in a Homopolymeric Nucleotide Repeat Common to uspA2H Genes
Infect. Immun., April 1, 2007; 75(4): 2035 - 2045.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
A. S. Attia and E. J. Hansen
A Conserved Tetranucleotide Repeat Is Necessary for Wild-Type Expression of the Moraxella catarrhalis UspA2 Protein
J. Bacteriol., November 15, 2006; 188(22): 7840 - 7852.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. M. Pearson, C. A. Laurence, S. E. Guinn, and E. J. Hansen
Biofilm Formation by Moraxella catarrhalis In Vitro: Roles of the UspA1 Adhesin and the Hag Hemagglutinin
Infect. Immun., March 1, 2006; 74(3): 1588 - 1596.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
T. Nordstrom, J. Jendholm, M. Samuelsson, A. Forsgren, and K. Riesbeck
The IgD-binding domain of the Moraxella IgD-binding protein MID (MID962-1200) activates human B cells in the presence of T cell cytokines
J. Leukoc. Biol., February 1, 2006; 79(2): 319 - 329.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Nordstrom, A. M. Blom, T. T. Tan, A. Forsgren, and K. Riesbeck
Ionic Binding of C3 to the Human Pathogen Moraxella catarrhalis Is a Unique Mechanism for Combating Innate Immunity
J. Immunol., September 15, 2005; 175(6): 3628 - 3636.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
B. Bullard, S. L. Lipski, and E. R. Lafontaine
Hag Directly Mediates the Adherence of Moraxella catarrhalis to Human Middle Ear Cells
Infect. Immun., August 1, 2005; 73(8): 5127 - 5136.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Nordstrom, A. M. Blom, A. Forsgren, and K. Riesbeck
The Emerging Pathogen Moraxella catarrhalis Interacts with Complement Inhibitor C4b Binding Protein through Ubiquitous Surface Proteins A1 and A2
J. Immunol., October 1, 2004; 173(7): 4598 - 4606.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. M. Holm, S. L. Vanlerberg, I. M. Foley, D. D. Sledjeski, and E. R. Lafontaine
The Moraxella catarrhalis Porin-Like Outer Membrane Protein CD Is an Adhesin for Human Lung Cells
Infect. Immun., April 1, 2004; 72(4): 1906 - 1913.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
P. Stutzmann Meier, N. Heiniger, R. Troller, and C. Aebi
Salivary Antibodies Directed against Outer Membrane Proteins of Moraxella catarrhalis in Healthy Adults
Infect. Immun., December 1, 2003; 71(12): 6793 - 6798.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. M. Holm, S. L. Vanlerberg, D. D. Sledjeski, and E. R. Lafontaine
The Hag Protein of Moraxella catarrhalis Strain O35E Is Associated with Adherence to Human Lung and Middle Ear Cells
Infect. Immun., September 1, 2003; 71(9): 4977 - 4984.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. M. Timpe, M. M. Holm, S. L. Vanlerberg, V. Basrur, and E. R. Lafontaine
Identification of a Moraxella catarrhalis Outer Membrane Protein Exhibiting Both Adhesin and Lipolytic Activities
Infect. Immun., August 1, 2003; 71(8): 4341 - 4350.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. Forsgren, M. Brant, M. Karamehmedovic, and K. Riesbeck
The Immunoglobulin D-Binding Protein MID from Moraxella catarrhalis Is Also an Adhesin
Infect. Immun., June 1, 2003; 71(6): 3302 - 3309.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
A. Mollenkvist, T. Nordstrom, C. Hallden, J. J. Christensen, A. Forsgren, and K. Riesbeck
The Moraxella catarrhalis Immunoglobulin D-Binding Protein MID Has Conserved Sequences and Is Regulated by a Mechanism Corresponding to Phase Variation
J. Bacteriol., April 1, 2003; 185(7): 2285 - 2295.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Nordstrom, A. Forsgren, and K. Riesbeck
The Immunoglobulin D-binding Part of the Outer Membrane Protein MID from Moraxella catarrhalis Comprises 238 Amino Acids and a Tetrameric Structure
J. Biol. Chem., September 13, 2002; 277(38): 34692 - 34699.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. M. Pearson, E. R. Lafontaine, N. J. Wagner, J. W. St. Geme III, and E. J. Hansen
A hag Mutant of Moraxella catarrhalis Strain O35E Is Deficient in Hemagglutination, Autoagglutination, and Immunoglobulin D-Binding Activities
Infect. Immun., August 1, 2002; 70(8): 4523 - 4533.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. G. Wingren, R. Hadzic, A. Forsgren, and K. Riesbeck
The Novel IgD Binding Protein from Moraxella catarrhalis Induces Human B Lymphocyte Activation and Ig Secretion in the Presence of Th2 Cytokines
J. Immunol., June 1, 2002; 168(11): 5582 - 5588.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.