| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Protein Chemistry Laboratory, Department of Pathology and Laboratory Medicine, and
Department of Medicine, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104; and
National Centre for Cell Science, Pune University Campus, Ganeshkhind, Pune, India
The molecular mechanisms involved in the interaction of complement receptor 2 (CR2) with its natural ligands iC3b and C3d are still not well understood. In addition, studies regarding the binding site(s) of the receptor on C3 as well as the affinities of the C3 fragments for CR2 have produced contradictory results. In the present study, we have used surface plasmon resonance technology to study the interaction of CR2 with its ligands C3d, iC3b, and the EBV surface glycoprotein gp350/220. We measured the kinetics of binding of the receptor to its ligands, examined the influence of ionic contacts on these interactions, and assessed whether immobilized and soluble iC3b bound with similar kinetics to CR2. Our results indicate that 1) gp350 binding to CR2 follows a simple 1:1 interaction, whereas that of the C3 fragments is more complex and involves more than one intramolecular component; 2) kinetic differences exist between the binding of C3d and iC3b to CR2, which may be due to an additional binding site found on the C3c region of iC3b; and 3) iC3b binds to CR2 with different kinetics, depending on whether the iC3b is in solution or immobilized on the surface. These findings suggest that binding of CR2 to iC3b and C3d is more complex than previously thought.
This article has been cited by other articles:
|
|
 |
|
  J. M. Kovacs, J. P. Hannan, E. Z. Eisenmesser, and V. M. Holers Mapping of the C3d Ligand Binding Site on Complement Receptor 2 (CR2/CD21) Using Nuclear Magnetic Resonance and Chemical Shift Analysis J. Biol. Chem., April 3, 2009; 284(14): 9513 - 9520. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Ricklin, S. K. Ricklin-Lichtsteiner, M. M. Markiewski, B. V. Geisbrecht, and J. D. Lambris Cutting Edge: Members of the Staphylococcus aureus Extracellular Fibrinogen-Binding Protein Family Inhibit the Interaction of C3d with Complement Receptor 2 J. Immunol., December 1, 2008; 181(11): 7463 - 7467. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Young, A. P. Herbert, P. N. Barlow, V. M. Holers, and J. P. Hannan Molecular Basis of the Interaction between Complement Receptor Type 2 (CR2/CD21) and Epstein-Barr Virus Glycoprotein gp350 J. Virol., November 15, 2008; 82(22): 11217 - 11227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Young, X. S. Chen, V. M. Holers, and J. P. Hannan Isolating the Epstein-Barr Virus gp350/220 Binding Site on Complement Receptor Type 2 (CR2/CD21) J. Biol. Chem., December 14, 2007; 282(50): 36614 - 36625. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Asokan, J. Hua, K. A. Young, H. J. Gould, J. P. Hannan, D. M. Kraus, G. Szakonyi, G. J. Grundy, X. S. Chen, M. K. Crow, et al. Characterization of Human Complement Receptor Type 2 (CR2/CD21) as a Receptor for IFN-{alpha}: A Potential Role in Systemic Lupus Erythematosus J. Immunol., July 1, 2006; 177(1): 383 - 394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Urquiza, R. Lopez, H. Patino, J. E. Rosas, and M. E. Patarroyo Identification of Three gp350/220 Regions Involved in Epstein-Barr Virus Invasion of Host Cells J. Biol. Chem., October 21, 2005; 280(42): 35598 - 35605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Sfyroera, M. Katragadda, D. Morikis, S. N. Isaacs, and J. D. Lambris Electrostatic Modeling Predicts the Activities of Orthopoxvirus Complement Control Proteins J. Immunol., February 15, 2005; 174(4): 2143 - 2151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bernet, J. Mullick, Y. Panse, P. B. Parab, and A. Sahu Kinetic Analysis of the Interactions between Vaccinia Virus Complement Control Protein and Human Complement Proteins C3b and C4b J. Virol., September 1, 2004; 78(17): 9446 - 9457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Morikis and J. D. Lambris The Electrostatic Nature of C3d-Complement Receptor 2 Association J. Immunol., June 15, 2004; 172(12): 7537 - 7547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. V. Barrault and A. M. Knight Distinct Sequences in the Cytoplasmic Domain of Complement Receptor 2 Are Involved in Antigen Internalization and Presentation J. Immunol., March 15, 2004; 172(6): 3509 - 3517. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
