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Cutting Edge |
2 Integrin, Very Late Antigen-2)1
*
Department of Microbiology and Immunology and Cancer Research Institute, University of California, San Francisco, CA 94143;
Department of Molecular Genetics, Chiba University Graduate School of Medicine, Chiba, Japan; and
Department of Immunology, DNAX Research Institute, Palo Alto, CA 94304
DX5 mAb is a useful reagent because it stains NK cells from all
mouse strains examined. We have identified the molecule recognized by
DX5 mAb by using a retrovirus-mediated expression cloning system. A
5-kb cDNA encoding a protein that is reactive with the DX5 mAb was
isolated from a NK cell cDNA library, and this molecule was identical
with CD49b (very late Ag-2, 
2 integrin). The DX5 mAb
reacted with transfectants expressing CD49b, and binding of DX5 to the
NK cells and CD49b transfectants was blocked in the presence of other
anti-CD49b mAbs. When NK1.1+ NK cells were cultured
with IL-2, they progressively lost reactivity with DX5 mAb as a
consequence of cellular proliferation. Cytotoxicity mediated by the
DX5+ NK cells was dramatically higher as compared with
DX5- NK cells. Therefore, DX5 mAb recognizes CD49b and can
be used to define functionally distinct subsets of NK
cells.
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