| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Medical Research Council Centre for Inflammation Research, University of Edinburgh Medical School, Edinburgh, United Kingdom; and
School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, United Kingdom
Phagocytic clearance of apoptotic granulocytes has a pivotal role in determining an inflammatory outcome, resolution or progression to a chronic state associated with development of fibrotic repair mechanisms, and/or autoimmune responses. In this study, we describe reprogramming of monocyte to macrophage differentiation by glucocorticoids, resulting in a marked augmentation of their capacity for phagocytosis of apoptotic neutrophils. This monocyte/macrophage phenotype was characterized by decreased phosphorylation, and therefore recruitment of paxillin and pyk2 to focal contacts and a down-regulation of p130Cas, a key adaptor molecule in integrin adhesion signaling. Glucocorticoid-treated cells also displayed higher levels of active Rac and cytoskeletal activity, which were mirrored by increases in phagocytic capability for apoptotic neutrophils. We propose that changes in the capacity for reorganization of cytoskeletal elements induced by glucocorticoids are essential for efficient phagocytic uptake of apoptotic cells.
This article has been cited by other articles:
|
|
 |
|
  C.-Y. Bai, M. Ohsugi, Y. Abe, and T. Yamamoto ZRP-1 controls Rho GTPase-mediated actin reorganization by localizing at cell-matrix and cell-cell adhesions J. Cell Sci., August 15, 2007; 120(16): 2828 - 2837. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mitchell, S. J. O'Meara, A. Gaffney, J. K. G. Crean, B. T. Kinsella, and C. Godson The Lipoxin A4 Receptor Is Coupled to SHP-2 Activation: IMPLICATIONS FOR REGULATION OF RECEPTOR TYROSINE KINASES J. Biol. Chem., May 25, 2007; 282(21): 15606 - 15618. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. N. Serhan, S. D. Brain, C. D. Buckley, D. W. Gilroy, C. Haslett, L. A. J. O'Neill, M. Perretti, A. G. Rossi, and J. L. Wallace Resolution of inflammation: state of the art, definitions and terms FASEB J, February 1, 2007; 21(2): 325 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ehrchen, L. Steinmuller, K. Barczyk, K. Tenbrock, W. Nacken, M. Eisenacher, U. Nordhues, C. Sorg, C. Sunderkotter, and J. Roth Glucocorticoids induce differentiation of a specifically activated, anti-inflammatory subtype of human monocytes Blood, February 1, 2007; 109(3): 1265 - 1274. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Gilmour, A. E. Coutinho, J.-F. Cailhier, T. Y. Man, M. Clay, G. Thomas, H. J. Harris, J. J. Mullins, J. R. Seckl, J. S. Savill, et al. Local amplification of glucocorticoids by 11beta-hydroxysteroid dehydrogenase type 1 promotes macrophage phagocytosis of apoptotic leukocytes. J. Immunol., June 15, 2006; 176(12): 7605 - 7611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. W. Vandivier, P. M. Henson, and I. S. Douglas Burying the Dead: The Impact of Failed Apoptotic Cell Removal (Efferocytosis) on Chronic Inflammatory Lung Disease Chest, June 1, 2006; 129(6): 1673 - 1682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. A. Patel, A. Longacre, K. Hsiao, H. Fan, F. Meng, J. E. Mitchell, J. Rauch, D. S. Ucker, and J. S. Levine Apoptotic Cells, at All Stages of the Death Process, Trigger Characteristic Signaling Events That Are Divergent from and Dominant over Those Triggered by Necrotic Cells: IMPLICATIONS FOR THE DELAYED CLEARANCE MODEL OF AUTOIMMUNITY J. Biol. Chem., February 24, 2006; 281(8): 4663 - 4670. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S W Tas, P Quartier, M Botto, and L Fossati-Jimack Macrophages from patients with SLE and rheumatoid arthritis have defective adhesion in vitro, while only SLE macrophages have impaired uptake of apoptotic cells Ann Rheum Dis, February 1, 2006; 65(2): 216 - 221. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-L. N. Huynh, K. C. Malcolm, C. Kotaru, J. A. Tilstra, J. Y. Westcott, V. A. Fadok, and S. E. Wenzel Defective Apoptotic Cell Phagocytosis Attenuates Prostaglandin E2 and 15-Hydroxyeicosatetraenoic Acid in Severe Asthma Alveolar Macrophages Am. J. Respir. Crit. Care Med., October 15, 2005; 172(8): 972 - 979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Maderna, S. Yona, M. Perretti, and C. Godson Modulation of Phagocytosis of Apoptotic Neutrophils by Supernatant from Dexamethasone-Treated Macrophages and Annexin-Derived Peptide Ac2-26 J. Immunol., March 15, 2005; 174(6): 3727 - 3733. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. McHugh, S. A. Krause, B. Yu, A.-M. Deans, S. Heasman, P. McLaughlin, and M. M.S. Heck Invadolysin: a novel, conserved metalloprotease links mitotic structural rearrangements with cell migration J. Cell Biol., November 22, 2004; 167(4): 673 - 686. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Jiang and D. S. Pisetsky The Effect of Dexamethasone on the Generation of Plasma DNA from Dead and Dying Cells Am. J. Pathol., May 1, 2004; 164(5): 1751 - 1759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Mitchell, G. Thomas, K. Harvey, D. Cottell, K. Reville, G. Berlasconi, N. A. Petasis, L. Erwig, A. J. Rees, J. Savill, et al. Lipoxins, Aspirin-Triggered Epi-Lipoxins, Lipoxin Stable Analogues, and the Resolution of Inflammation: Stimulation of Macrophage Phagocytosis of Apoptotic Neutrophils In Vivo J. Am. Soc. Nephrol., October 1, 2002; 13(10): 2497 - 2507. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
