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/CD8 Interaction on the Surface of T Cells
Department of Immunology, Mayo Graduate and Medical Schools, Mayo Clinic, Rochester, MN 55905
Both CD8 and the TCR bind to MHC class I molecules during
physiologic T cell activation. It has been shown that for optimal T
cell activation to occur, CD8 must be able to bind the same class I
molecule that is bound by the TCR. However, no direct evidence for the
class I-dependent association of CD8 and the TCR has been demonstrated.
Using fluorescence resonance energy transfer, we show directly that a
single class I molecule causes TCR/CD8 interaction by serving as a
docking molecule for both CD8 and the TCR. Furthermore, we show that
CD3
is brought into close proximity with CD8 upon TCR/CD8
association. These interactions are not dependent on the
phosphorylation events characteristic of T cell activation. Thus, MHC
class I molecules, by binding to both CD8 and the TCR, mediate the
reorganization of T cell membrane components to promote cellular
activation.
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