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*
Department of Immunology, School of Life Science, Faculty of Medicine, Tottori University, Yonago, Japan;
Laboratory of Pluripotent Cell Studies, RIKEN Center for Developmental Biology, Kobe, Japan; and
Center for Molecular and Developmental Biology, Graduate School of Science, Kyoto University, Kyoto, Japan
Hemopoietic cells develop in a complex milieu that is made up of
diverse components, including stromal cells. Wnt genes,
which are known to regulate the fate of the cells in a variety of
tissues, are expressed in hemopoietic organs. However, their roles in
hemopoiesis are not well characterized. In this study, we examined the
roles of Wnt proteins in hemopoiesis using conditioned medium
containing Wnt-3a. This conditioned medium dramatically reduced the
production of B lineage cells and myeloid lineage cells, except for
macrophages in the long-term bone marrow cultures grown on stromal
cells, although the sensitivity to the conditioned medium differed,
depending on the hemopoietic lineage. In contrast, the same conditioned
medium did not affect the generation of B lineage or myeloid lineage
cells in stromal cell-free conditions. These results suggested that Wnt
proteins exert their effects through stromal cells. Indeed, these
effects were mimicked by the expression of a stabilized form of
-catenin in stromal cells. In this study, we demonstrated that Wnt
signaling regulates hemopoiesis through stromal cells with selectivity
and different degrees of the effect, depending on the hemopoietic
lineage in the hemopoietic microenvironment.
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