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*
Division of Immunology, Aichi Cancer Center Research Institute, Nagoya, Japan; and
Second Department of Pathology, Aichi Medical University, Nagakute, Japan
Thymus leukemia (TL) Ags belong to the family of nonclassical MHC
class I Ags and can be recognized by both TCR
and TCR
CTL
with TL, but not H-2 restriction. We previously reported that the CTL
epitope is TAP independent, but the antigenic molecule(s) presented by
TL has yet to be determined. In the present study, TL tetramers were
prepared with T3b-TL and murine
2-microglobulin, not including antigenic peptides, and
binding specificity was studied. CTL clones against TL Ags were stained
with the T3b-TL tetramer, and the binding shown to be CD3
and CD8 dependent. Normal lymphocytes from various origins were also
studied. Surprisingly, most CD8+ intraepithelial
lymphocytes derived from the small intestines (iIEL), as well as
CD8+ and CD4+CD8+ thymocytes, were
stained, while only very minor populations of CD8+ cells
derived from other peripheral lymphoid tissues, such as spleen and
lymph nodes, were positive. The binding of T3b-TL tetramers
to CD8+ iIEL and thymocytes was CD8 dependent, but CD3
independent, in contrast to that to TL-restricted CTL. These results
altogether showed that TL-restricted CTL can be monitored by
CD3-dependent binding of T3b-TL tetramers. In addition,
CD3-independent T3b-TL tetramer binding to iIEL and
thymocytes may imply that TL expressed on intestinal epithelium and
cortical thymocytes has a physiological function interacting with these
tetramer+CD8+ T
lymphocytes.
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