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CUTTING EDGE |
Expression Is Critical for Export of IL-2 from T Cells1
Departments of
*
Biochemistry and
Pharmacology, Royal College of Surgeons in Ireland, and
Department of Clinical Medicine, Trinity Centre for Health Sciences, St. James’s Hospital, Dublin, Ireland.
Protein kinase C (PKC) plays an integral part in T cell
activation and IL-2 secretion. We investigated the role of a particular
PKC isoform, PKC
, in IL-2 production and secretion. The T cell
lymphoma line HuT 78 secretes IL-2 in response to the phorbol ester
PMA. A PKC-deficient clone of HuT 78, K-4, did not secrete IL-2 in
response to PMA stimulation. As assessed by RT-PCR, K-4 expressed mRNA
for IL-2 following PMA activation, and intracellular IL-2 protein was
detected by immunofluorescence. An enhanced green fluorescent
protein-linked PKC construct was microinjected into K-4 cells, which
were then stimulated with PMA; those cells that expressed PKC could
secrete IL-2, as determined by an in situ immunofluorescent assay. This
study demonstrates that PKC is not necessary for transcription of
the IL-2 gene or translation of mRNA to protein, but that expression of
this PKC isoform is critical to the export of IL-2 molecules from T
cells.
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