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1.2 Segment to the Recognition of Nonpeptide Antigens by Human 
T Cells1
*
Department of Immunology and Cell Biology, Graduate School of Medicine, and
Graduate School of Biostudies, Kyoto University, Kyoto, Japan;
Department of Dermatology, Shiga University of Medical Science, Ohtsu, Shiga, Japan; and
Department of New Food Design, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto, Japan
Human 
T cells display unique repertoires of Ag specificities
largely imposed by selective usages of distinct V and V genes.
Among them, V2/V2+ T cells predominate in the
circulation of healthy adults and respond to various microbial small
molecular mass nonpeptide Ags. The present results indicate that the
primary V2/V2+ T cells stimulated with the distinct
groups of nonpeptide Ags, including monoethyl pyrophosphate, isobutyl
amine, and aminobisphosphonate, invariably exhibit J1.2 in the
V2+ TCR- chains. Gene transfer studies revealed that
most of the randomly cloned V2/J1.2+ TCR- genes
bearing diverse V/J junctional sequences could confer the
responsiveness to all these nonpeptide Ags, while none of the
V2/J1.1+ or V2/J1.3+ TCR- genes
could do so. Furthermore, mutation of the lysine residues encoded by
the J1.2 gene, which are unique in human J1.2 and absent in other
human or mouse J segments, completely abrogated the responsiveness
to all the nonpeptide Ags without affecting the response to
anti-CD3 mAb. These results strongly suggested that the positively
charged lysine residues in the TCR- chain CDR3 region encoded by the
germline J1.2 gene play a key role in the recognition of diverse
small molecular mass nonpeptide Ags.
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