The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Monleón, I.
Right arrow Articles by Anel, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Monleón, I.
Right arrow Articles by Anel, A.
The Journal of Immunology, 2001, 167: 6736-6744.
Copyright © 2001 by The American Association of Immunologists

Differential Secretion of Fas Ligand- or APO2 Ligand/TNF-Related Apoptosis-Inducing Ligand-Carrying Microvesicles During Activation-Induced Death of Human T Cells1

Inmaculada Monleón2,*, María José Martínez-Lorenzo2,{dagger}, Luis Monteagudo{ddagger}, Pilar Lasierra{dagger}, Marta Taulés§, María Iturralde*, Andrés Piñeiro*, Luis Larrad{dagger}, María Angeles Alava*, Javier Naval* and Alberto Anel3,*

* Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, {dagger} Servicio de Inmunología, Hospital Clínico Universitario, and {ddagger} Departamento de Anatomía, Embriología y Genética, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain; and § Serveis Científico-Tècnics, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain

Preformed Fas ligand (FasL) and APO2 ligand (APO2L)/TNF-related apoptosis-inducing ligand (TRAIL) are stored in the cytoplasm of the human Jurkat T cell line and of normal human T cell blasts. The rapid release of these molecules in their bioactive form is involved in activation-induced cell death. In this study, we show by confocal microscopy that FasL and APO2L/TRAIL are mainly localized in lysosomal-like compartments in these cells. We show also by immunoelectron microscopy that FasL and APO2L/TRAIL are stored inside cytoplasmic compartments ~500 nm in diameter, with characteristics of multivesicular bodies. Most of these compartments share FasL and APO2L/TRAIL, although exclusive APO2L/TRAIL labeling can be also observed in separate compartments. Upon PHA activation, the mobilization of these compartments toward the plasma membrane is evident, resulting in the secretion of the internal microvesicles loaded with FasL and APO2L/TRAIL. In the case of activation with anti-CD59 mAb, the secretion of microvesicles labeled preferentially with APO2L/TRAIL predominates. These data provide the basis of a new and efficient mechanism for the rapid induction of autocrine or paracrine cell death during immune regulation and could modify the interpretation of the role of FasL and APO2L/TRAIL as effector mechanisms in physiological and pathological situations.




This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
A. Bosque, J. I. Aguilo, M. del Rey, E. Paz-Artal, L. M. Allende, J. Naval, and A. Anel
Cell cycle regulation by FasL and Apo2L/TRAIL in human T-cell blasts. Implications for autoimmune lymphoproliferative syndromes
J. Leukoc. Biol., August 1, 2008; 84(2): 488 - 498.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
N. Gasper-Smith, D. M. Crossman, J. F. Whitesides, N. Mensali, J. S. Ottinger, S. G. Plonk, M. A. Moody, G. Ferrari, K. J. Weinhold, S. E. Miller, et al.
Induction of Plasma (TRAIL), TNFR-2, Fas Ligand, and Plasma Microparticles after Human Immunodeficiency Virus Type 1 (HIV-1) Transmission: Implications for HIV-1 Vaccine Design
J. Virol., August 1, 2008; 82(15): 7700 - 7710.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. M. Keller, T. A. Groothuis, E. A. M. Veraar, M. Marsman, L. M. de Buy Wenniger, H. Janssen, J. Neefjes, and J. Borst
Costimulatory ligand CD70 is delivered to the immunological synapse by shared intracellular trafficking with MHC class II molecules
PNAS, April 3, 2007; 104(14): 5989 - 5994.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
R. Valenti, V. Huber, M. Iero, P. Filipazzi, G. Parmiani, and L. Rivoltini
Tumor-Released Microvesicles as Vehicles of Immunosuppression
Cancer Res., April 1, 2007; 67(7): 2912 - 2915.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. Bosque, J. I. Aguilo, M. A. Alava, E. Paz-Artal, J. Naval, L. M. Allende, and A. Anel
The induction of Bim expression in human T-cell blasts is dependent on nonapoptotic Fas/CD95 signaling
Blood, February 15, 2007; 109(4): 1627 - 1635.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E. Zuccato, E. J. Blott, O. Holt, S. Sigismund, M. Shaw, G. Bossi, and G. M. Griffiths
Sorting of Fas ligand to secretory lysosomes is regulated by mono-ubiquitylation and phosphorylation
J. Cell Sci., January 1, 2007; 120(1): 191 - 199.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Del-Rey, J. Ruiz-Contreras, A. Bosque, S. Calleja, J. Gomez-Rial, E. Roldan, P. Morales, A. Serrano, A. Anel, E. Paz-Artal, et al.
A homozygous Fas ligand gene mutation in a patient causes a new type of autoimmune lymphoproliferative syndrome
Blood, August 15, 2006; 108(4): 1306 - 1312.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
S. J. Blossom and K. M. Gilbert
Exposure to a Metabolite of the Environmental Toxicant, Trichloroethylene, Attenuates CD4+ T Cell Activation-Induced Cell Death by Metalloproteinase-Dependent FasL Shedding
Toxicol. Sci., July 1, 2006; 92(1): 103 - 114.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. D. Taylor, S. Akyol, and C. Gercel-Taylor
Pregnancy-Associated Exosomes and Their Modulation of T Cell Signaling
J. Immunol., February 1, 2006; 176(3): 1534 - 1542.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Alonso, M. C. Rodriguez, J. Pindado, E. Merino, I. Merida, and M. Izquierdo
Diacylglycerol Kinase {alpha} Regulates the Secretion of Lethal Exosomes Bearing Fas Ligand during Activation-induced Cell Death of T Lymphocytes
J. Biol. Chem., August 5, 2005; 280(31): 28439 - 28450.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
M N Lub-de Hooge, E G E de Vries, S de Jong, and M Bijl
Soluble TRAIL concentrations are raised in patients with systemic lupus erythematosus
Ann Rheum Dis, June 1, 2005; 64(6): 854 - 858.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
J. M. Martin-Fernandez, J. A. Cabanillas, M. Rivero-Carmena, E. Lacasa, J. Pardo, A. Anel, P. R. Ramirez-Duque, F. Merino, C. Rodriguez-Gallego, and J. R. Regueiro
Herpesvirus saimiri-transformed CD8+ T cells as a tool to study Chediak-Higashi syndrome cytolytic lymphocytes
J. Leukoc. Biol., May 1, 2005; 77(5): 661 - 668.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
A. Bosque, J. Pardo, M{a} J. Martinez-Lorenzo, M. Iturralde, I. Marzo, A. Pineiro, M{a} A. Alava, J. Naval, and A. Anel
Down-regulation of normal human T cell blast activation: roles of APO2L/TRAIL, FasL, and c- FLIP, Bim, or Bcl-x isoform expression
J. Leukoc. Biol., April 1, 2005; 77(4): 568 - 578.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
S. Okuyama, A. Komatsuda, H. Wakui, N. Aiba, N. Fujishima, K. Iwamoto, H. Ohtani, and K.-i. Sawada
Up-regulation of TRAIL mRNA expression in peripheral blood mononuclear cells from patients with minimal-change nephrotic syndrome
Nephrol. Dial. Transplant., March 1, 2005; 20(3): 539 - 544.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
L. Frangsmyr, V. Baranov, O. Nagaeva, U. Stendahl, L. Kjellberg, and L. Mincheva-Nilsson
Cytoplasmic microvesicular form of Fas ligand in human early placenta: switching the tissue immune privilege hypothesis from cellular to vesicular level
Mol. Hum. Reprod., January 1, 2005; 11(1): 35 - 41.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
C. Tecchio, V. Huber, P. Scapini, F. Calzetti, D. Margotto, G. Todeschini, L. Pilla, G. Martinelli, G. Pizzolo, L. Rivoltini, et al.
IFN{alpha}-stimulated neutrophils and monocytes release a soluble form of TNF-related apoptosis-inducing ligand (TRAIL/Apo-2 ligand) displaying apoptotic activity on leukemic cells
Blood, May 15, 2004; 103(10): 3837 - 3844.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
Y. Vera, M. Diaz-Romero, S. Rodriguez, Y. Lue, C. Wang, R. S. Swerdloff, and A. P. Sinha Hikim
Mitochondria-Dependent Pathway Is Involved in Heat-Induced Male Germ Cell Death: Lessons from Mutant Mice
Biol Reprod, May 1, 2004; 70(5): 1534 - 1540.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
V. M. Abrahams, S. L. Straszewski-Chavez, S. Guller, and G. Mor
First trimester trophoblast cells secrete Fas ligand which induces immune cell apoptosis
Mol. Hum. Reprod., January 1, 2004; 10(1): 55 - 63.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
Y. Yang, I. Tikhonov, T. J. Ruckwardt, M. Djavani, J. C. Zapata, C. D. Pauza, and M. S. Salvato
Monocytes Treated with Human Immunodeficiency Virus Tat Kill Uninfected CD4+ Cells by a Tumor Necrosis Factor-Related Apoptosis-Induced Ligand-Mediated Mechanism
J. Virol., June 15, 2003; 77(12): 6700 - 6708.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. Fritzsching, B. Schwer, J. Kartenbeck, A. Pedal, V. Horejsi, and M. Ott
Release and Intercellular Transfer of Cell Surface CD81 Via Microparticles
J. Immunol., November 15, 2002; 169(10): 5531 - 5537.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. Dorothee, I. Vergnon, J. Menez, H. Echchakir, D. Grunenwald, M. Kubin, S. Chouaib, and F. Mami-Chouaib
Tumor-Infiltrating CD4+ T Lymphocytes Express APO2 Ligand (APO2L)/TRAIL upon Specific Stimulation with Autologous Lung Carcinoma Cells: Role of IFN-{alpha} on APO2L/TRAIL Expression and -Mediated Cytotoxicity
J. Immunol., July 15, 2002; 169(2): 809 - 817.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.