HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Amanna, I. J. Articles by Hayes, C. E. Search for Related Content PubMed PubMed Citation Articles by Amanna, I. J. Articles by Hayes, C. E.

Cutting Edge: A/WySnJ Transitional B Cells Overexpress the Chromosome 15 Proapoptotic Blk Gene and Succumb to Premature Apoptosis¹

Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, WI 53706

Better knowledge of peripheral B lymphocyte homeostasis is needed to address the human hypogammaglobulinemia diseases. A defect in the Bcmd gene shortens the B cell life span and causes B cell deficiency in A/WySnJ mice. Previous genetic mapping placed Bcmd near Srebf2 on chromosome 15. Inspection of the human chromosome 22 syntenic region identified the proapoptotic Bik gene as a candidate. Two mapping methods placed the homologous mouse gene, Blk, near Srebf2. The Blk genomic structure was highly homologous to Bik. Sequence analysis ruled out coding region mutations, but Blk transcripts were overly abundant in sorted A/WySnJ T1 B cells. Moreover, enriched transitional B cells showed a cell-autonomous defect leading to excessive apoptosis. Thus, Bcmd may be a direct mutation in Blk, or in a gene involved in Blk regulation, such that excess expression pushes the A/WySnJ transitional B cells past the apoptosis checkpoint to cell death.

This article has been cited by other articles:

				E. Di Carlo, T. D'Antuono, P. Pompa, R. Giuliani, S. Rosini, L. Stuppia, P. Musiani, and C. Sorrentino The Lack of Epithelial Interleukin-7 and BAFF/BLyS Gene Expression in Prostate Cancer as a Possible Mechanism of Tumor Escape from Immunosurveillance Clin. Cancer Res., May 1, 2009; 15(9): 2979 - 2987. [Abstract] [Full Text] [PDF]

				E. C. Bridgeford, J. G. Fox, P. R. Nambiar, and A. B. Rogers Agammaglobulinemia and Staphylococcus aureus Botryomycosis in a Cohort of Related Sentinel Swiss Webster Mice J. Clin. Microbiol., May 1, 2008; 46(5): 1881 - 1884. [Abstract] [Full Text] [PDF]

				A. Craxton, K. E. Draves, A. Gruppi, and E. A. Clark BAFF regulates B cell survival by downregulating the BH3-only family member Bim via the ERK pathway J. Exp. Med., November 21, 2005; 202(10): 1363 - 1374. [Abstract] [Full Text] [PDF]

				J. P. Mathai, M. Germain, and G. C. Shore BH3-only BIK Regulates BAX,BAK-dependent Release of Ca2+ from Endoplasmic Reticulum Stores and Mitochondrial Apoptosis during Stress-induced Cell Death J. Biol. Chem., June 24, 2005; 280(25): 23829 - 23836. [Abstract] [Full Text] [PDF]

				L. Coultas, P. Bouillet, E. G. Stanley, T. C. Brodnicki, J. M. Adams, and A. Strasser Proapoptotic BH3-Only Bcl-2 Family Member Bik/Blk/Nbk Is Expressed in Hemopoietic and Endothelial Cells but Is Redundant for Their Programmed Death Mol. Cell. Biol., February 15, 2004; 24(4): 1570 - 1581. [Abstract] [Full Text] [PDF]

				I. J. Amanna, J. P. Dingwall, and C. E. Hayes Enforced bcl-xL Gene Expression Restored Splenic B Lymphocyte Development in BAFF-R Mutant Mice J. Immunol., May 1, 2003; 170(9): 4593 - 4600. [Abstract] [Full Text] [PDF]