The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Capowski, E. E.
Right arrow Articles by Malter, J. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Capowski, E. E.
Right arrow Articles by Malter, J. S.
The Journal of Immunology, 2001, 167: 5970-5976.
Copyright © 2001 by The American Association of Immunologists

Y Box-Binding Factor Promotes Eosinophil Survival by Stabilizing Granulocyte-Macrophage Colony-Stimulating Factor mRNA1

Elizabeth E. Capowski2, Stéphane Esnault2, Saswati Bhattacharya and James S. Malter3

Department of Pathology and Laboratory Medicine, University of Wisconsin Medical School, Madison, WI 53792

Short-lived peripheral blood eosinophils are recruited to the lungs of asthmatics after allergen challenge, where they become long-lived effector cells central to disease pathophysiology. GM-CSF is an important cytokine which promotes eosinophil differentiation, function, and survival after transit into the lung. In human eosinophils, GM-CSF production is controlled by regulated mRNA stability mediated by the 3' untranslated region, AU-rich elements (ARE). We identified human Y box-binding factor 1 (YB-1) as a GM-CSF mRNA ARE-specific binding protein that is capable of enhancing GM-CSF-dependent survival of eosinophils. Using a transfection system that mimics GM-CSF metabolism in eosinophils, we have shown that transduced YB-1 stabilized GM-CSF mRNA in an ARE-dependent mechanism, causing increased GM-CSF production and enhanced in vitro survival. RNA EMSAs indicate that YB-1 interacts with the GM-CSF mRNA through its 3' untranslated region ARE. In addition, endogenous GM-CSF mRNA coimmunoprecipitates with endogenous YB-1 protein in activated eosinophils but not resting cells. Thus, we propose a model whereby activation of eosinophils leads to YB-1 binding to and stabilization of GM-CSF mRNA, ultimately resulting in GM-CSF release and prolonged eosinophil survival.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
T. Rauen, U. Raffetseder, B. C. Frye, S. Djudjaj, P. J. T. Muhlenberg, F. Eitner, U. Lendahl, J. Bernhagen, S. Dooley, and P. R. Mertens
YB-1 Acts as a Ligand for Notch-3 Receptors and Modulates Receptor Activation
J. Biol. Chem., September 25, 2009; 284(39): 26928 - 26940.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
R. Krohn, U. Raffetseder, I. Bot, A. Zernecke, E. Shagdarsuren, E. A. Liehn, P. J. van Santbrink, P. J. Nelson, E. A. Biessen, P. R. Mertens, et al.
Y-Box Binding Protein-1 Controls CC Chemokine Ligand-5 (CCL5) Expression in Smooth Muscle Cells and Contributes to Neointima Formation in Atherosclerosis-Prone Mice
Circulation, October 16, 2007; 116(16): 1812 - 1820.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Esnault, Z.-J. Shen, E. Whitesel, and J. S. Malter
The Peptidyl-Prolyl Isomerase Pin1 Regulates Granulocyte-Macrophage Colony-Stimulating Factor mRNA Stability in T Lymphocytes
J. Immunol., November 15, 2006; 177(10): 6999 - 7006.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Dooley, H. M. Said, A. M. Gressner, J. Floege, A. En-Nia, and P. R. Mertens
Y-box Protein-1 Is the Crucial Mediator of Antifibrotic Interferon-{gamma} Effects
J. Biol. Chem., January 20, 2006; 281(3): 1784 - 1795.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
V. Evdokimova, P. Ruzanov, M. S. Anglesio, A. V. Sorokin, L. P. Ovchinnikov, J. Buckley, T. J. Triche, N. Sonenberg, and P. H. B. Sorensen
Akt-Mediated YB-1 Phosphorylation Activates Translation of Silent mRNA Species
Mol. Cell. Biol., January 1, 2006; 26(1): 277 - 292.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
C. R.C. van Roeyen, F. Eitner, S. Martinkus, S. R. Thieltges, T. Ostendorf, D. Bokemeyer, B. Luscher, J. M. Luscher-Firzlaff, J. Floege, and P. R. Mertens
Y-Box Protein 1 Mediates PDGF-B Effects in Mesangioproliferative Glomerular Disease
J. Am. Soc. Nephrol., October 1, 2005; 16(10): 2985 - 2996.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Zhang, X. Liu, J. G. Cogan, M. D. Fuerst, J. A. Polikandriotis, R. J. Kelm Jr., and A. R. Strauch
YB-1 Coordinates Vascular Smooth Muscle {alpha}-Actin Gene Activation by Transforming Growth Factor {beta}1 and Thrombin during Differentiation of Human Pulmonary Myofibroblasts
Mol. Biol. Cell, October 1, 2005; 16(10): 4931 - 4940.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Cornelis, S. A. Tinton, B. Schepens, Y. Bruynooghe, and R. Beyaert
UNR translation can be driven by an IRES element that is negatively regulated by polypyrimidine tract binding protein
Nucleic Acids Res., May 31, 2005; 33(10): 3095 - 3108.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
C. Stellato
Post-transcriptional and Nongenomic Effects of Glucocorticoids
Proceedings of the ATS, November 1, 2004; 1(3): 255 - 263.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. E. Bates, L. Y. Liu, S. Esnault, B. A. Stout, E. Fonkem, V. Kung, J. B. Sedgwick, E. A. B. Kelly, D. M. Bates, J. S. Malter, et al.
Expression of Interleukin-5- and Granulocyte Macrophage-Colony-Stimulating Factor-Responsive Genes in Blood and Airway Eosinophils
Am. J. Respir. Cell Mol. Biol., May 1, 2004; 30(5): 736 - 743.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Esnault and J. S. Malter
Hyaluronic Acid or TNF-{alpha} Plus Fibronectin Triggers Granulocyte Macrophage-Colony-Stimulating Factor mRNA Stabilization in Eosinophils Yet Engages Differential Intracellular Pathways and mRNA Binding Proteins
J. Immunol., December 15, 2003; 171(12): 6780 - 6787.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
U. Raffetseder, B. Frye, T. Rauen, K. Jurchott, H.-D. Royer, P. L. Jansen, and P. R. Mertens
Splicing Factor SRp30c Interaction with Y-box Protein-1 Confers Nuclear YB-1 Shuttling and Alternative Splice Site Selection
J. Biol. Chem., May 9, 2003; 278(20): 18241 - 18248.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
A. Skalweit, A. Doller, A. Huth, T. Kahne, P. B. Persson, and B.-J. Thiele
Posttranscriptional Control of Renin Synthesis: Identification of Proteins Interacting With Renin mRNA 3'-Untranslated Region
Circ. Res., March 7, 2003; 92(4): 419 - 427.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
H. Han, M. Fuortes, and C. Nathan
Critical Role of the Carboxyl Terminus of Proline-rich Tyrosine Kinase (Pyk2) in the Activation of Human Neutrophils by Tumor Necrosis Factor: Separation of Signals for the Respiratory Burst and Degranulation
J. Exp. Med., January 6, 2003; 197(1): 63 - 75.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. Esnault and J. S. Malter
Extracellular signal-regulated kinase mediates granulocyte-macrophage colony-stimulating factor messenger RNA stabilization in tumor necrosis factor-alpha plus fibronectin-activated peripheral blood eosinophils
Blood, May 13, 2002; 99(11): 4048 - 4052.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.