| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1
Departments of
*
Physiology and
Clinical Hematology, Osaka City University Medical School, Osaka, Japan
We investigated activation of mitogen-activated protein kinase
(MAPK) subtype cascades in human neutrophils stimulated by IL-1
.
IL-1 induced phosphorylation and activation of p38 MAPK and
phosphorylation of MAPK kinase-3/6 (MKK3/6). Maximal activation of p38
MAPK was obtained by stimulation of cells with 300 U/ml IL-1 for 10
min. Extracellular signal-regulated kinase (ERK) was faintly
phosphorylated and c-Jun N-terminal kinase (JNK) was not phosphorylated
by IL-1. IL-1 primed neutrophils for enhanced release of
superoxide (O2-) stimulated by FMLP in
parallel with increased phosphorylation of p38 MAPK. IL-1 also
induced O2- release and up-regulation of CD11b
and CD15, and both responses were inhibited by SB203580 (p38 MAPK
inhibitor), suggesting that p38 MAPK activation mediates
IL-1-induced O2- release and up-regulation
of CD11b and CD15. Combined stimulation of neutrophils with IL-1 and
G-CSF, a selective activator of the ERK cascade, resulted in the
additive effects when the priming effect and phosphorylation of p38
MAPK and ERK were assessed. IL-1 induced phosphorylation of ERK and
JNK as well as p38 MAPK in human endothelial cells. These findings
suggest that 1) in human neutrophils the MKK3/6-p38 MAPK cascade is
selectively activated by IL-1 and activation of this cascade
mediates IL-1-induced O2- release and
up-regulation of CD11b and CD15, and 2) the IL-1R-p38 MAPK pathway and
the G-CSF receptor-ERK pathway work independently for activation of
neutrophils.
This article has been cited by other articles:
|
|
 |
|
  P. Ehrenfeld, C. E. Matus, F. Pavicic, C. Toledo, F. Nualart, C. B. Gonzalez, R. A. Burgos, K. D. Bhoola, and C. D. Figueroa Kinin B1 receptor activation turns on exocytosis of matrix metalloprotease-9 and myeloperoxidase in human neutrophils: involvement of mitogen-activated protein kinase family J. Leukoc. Biol., November 1, 2009; 86(5): 1179 - 1189. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Gereben, A. M. Zavacki, S. Ribich, B. W. Kim, S. A. Huang, W. S. Simonides, A. Zeold, and A. C. Bianco Cellular and Molecular Basis of Deiodinase-Regulated Thyroid Hormone Signaling Endocr. Rev., December 1, 2008; 29(7): 898 - 938. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Katsube, T. Kato, M. Kitagawa, H. Noma, H. Fujita, and S. Kitagawa Calpain-mediated regulation of the distinct signaling pathways and cell migration in human neutrophils J. Leukoc. Biol., July 1, 2008; 84(1): 255 - 263. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Maeda, K. Soejima, K. Bandow, K. Kuroe, K. Kakimoto, S. Miyawaki, A. Okamoto, and T. Matsuguchi Force-induced IL-8 from Periodontal Ligament Cells Requires IL-1{beta} Journal of Dental Research, July 1, 2007; 86(7): 629 - 634. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cloutier, T. Ear, E. Blais-Charron, C. M. Dubois, and P. P. McDonald Differential involvement of NF-{kappa}B and MAP kinase pathways in the generation of inflammatory cytokines by human neutrophils J. Leukoc. Biol., February 1, 2007; 81(2): 567 - 577. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Alvarez, J. I. F. Bass, J. R. Geffner, P. X. F. Calotti, M. Costas, O. A. Coso, R. Gamberale, M. E. Vermeulen, G. Salamone, D. Martinez, et al. Neutrophil Signaling Pathways Activated by Bacterial DNA Stimulation J. Immunol., September 15, 2006; 177(6): 4037 - 4046. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Jinnouchi, Y. Aida, K. Nozoe, K. Maeda, and M. J. Pabst Local anesthetics inhibit priming of neutrophils by lipopolysaccharide for enhanced release of superoxide: suppression of cytochrome b558 expression by disparate mechanisms J. Leukoc. Biol., December 1, 2005; 78(6): 1356 - 1365. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Boelen, J. Kwakkel, A. Alkemade, R. Renckens, E. Kaptein, G. Kuiper, W. M. Wiersinga, and T. J. Visser Induction of Type 3 Deiodinase Activity in Inflammatory Cells of Mice with Chronic Local Inflammation Endocrinology, December 1, 2005; 146(12): 5128 - 5134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Harfi, F. Corazza, S. D'Hondt, and E. Sariban Differential Calcium Regulation of Proinflammatory Activities in Human Neutrophils Exposed to the Neuropeptide Pituitary Adenylate Cyclase-Activating Protein J. Immunol., September 15, 2005; 175(6): 4091 - 4102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Lokuta and A. Huttenlocher TNF-{alpha} promotes a stop signal that inhibits neutrophil polarization and migration via a p38 MAPK pathway J. Leukoc. Biol., July 1, 2005; 78(1): 210 - 219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Falasca, V. Iadevaia, F. Ciccosanti, G. Melino, A. Serafino, and M. Piacentini Transglutaminase Type II Is a Key Element in the Regulation of the Anti-Inflammatory Response Elicited by Apoptotic Cell Engulfment J. Immunol., June 1, 2005; 174(11): 7330 - 7340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Luo, D.-Q. Li, A. Doshi, W. Farley, R. M. Corrales, and S. C. Pflugfelder Experimental Dry Eye Stimulates Production of Inflammatory Cytokines and MMP-9 and Activates MAPK Signaling Pathways on the Ocular Surface Invest. Ophthalmol. Vis. Sci., December 1, 2004; 45(12): 4293 - 4301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.-Q. Li, Z. Chen, X. J. Song, L. Luo, and S. C. Pflugfelder Stimulation of Matrix Metalloproteinases by Hyperosmolarity via a JNK Pathway in Human Corneal Epithelial Cells Invest. Ophthalmol. Vis. Sci., December 1, 2004; 45(12): 4302 - 4311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Albelda, K. C. Lau, P. Chien, Z.-Y. Huang, E. Arguiris, A. Bohen, J. Sun, J. A. Billet, M. Christofidou-Solomidou, Z. K. Indik, et al. Role for Platelet-Endothelial Cell Adhesion Molecule-1 in Macrophage Fc{gamma} Receptor Function Am. J. Respir. Cell Mol. Biol., August 1, 2004; 31(2): 246 - 255. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nishiki, F. Hato, N. Kamata, E. Sakamoto, T. Hasegawa, A. Kimura-Eto, M. Hino, and S. Kitagawa Selective activation of STAT3 in human monocytes stimulated by G-CSF: implication in inhibition of LPS-induced TNF-{alpha} production Am J Physiol Cell Physiol, June 1, 2004; 286(6): C1302 - C1311. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kutsuna, K. Suzuki, N. Kamata, T. Kato, F. Hato, K. Mizuno, H. Kobayashi, M. Ishii, and S. Kitagawa Actin reorganization and morphological changes in human neutrophils stimulated by TNF, GM-CSF, and G-CSF: the role of MAP kinases Am J Physiol Cell Physiol, January 1, 2004; 286(1): C55 - C64. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cloutier, T. Ear, O. Borissevitch, P. Larivee, and P. P. McDonald Inflammatory Cytokine Expression Is Independent of the c-Jun N-Terminal Kinase/AP-1 Signaling Cascade in Human Neutrophils J. Immunol., October 1, 2003; 171(7): 3751 - 3761. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hasegawa, K. Suzuki, C. Sakamoto, K. Ohta, S. Nishiki, M. Hino, N. Tatsumi, and S. Kitagawa Expression of the inhibitor of apoptosis (IAP) family members in human neutrophils: up-regulation of cIAP2 by granulocyte colony-stimulating factor and overexpression of cIAP2 in chronic neutrophilic leukemia Blood, February 1, 2003; 101(3): 1164 - 1171. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
