| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
,
,
*
Molecular Biology Graduate Program and Departments of
Microbiology and
Internal Medicine, University of Iowa, Iowa City, IA 52242; and
Veterans Affairs Medical Center, Iowa City, IA 52242
Latent membrane protein 1 (LMP1) is an EBV-encoded transforming
protein that strongly mimics the B cell-activating properties of a
normal cellular membrane protein, CD40. LMP1 and CD40 both associate
with the cytoplasmic adapter proteins called TNFR-associated factors
(TRAFs). TRAFs 1, 2, and 3 bind to a region of LMP1 that is essential
for EBV to transform B lymphocytes, carboxyl-terminal activating region
(CTAR) 1. However, studies of transiently overexpressed LMP1 molecules,
primarily in epithelial cells, indicated that a second region, CTAR2,
is largely responsible for LMP1-mediated activation of NF-
B and
c-Jun N-terminal kinase. To better understand LMP1 signaling in
B lymphocytes, we performed a structure-function analysis of the LMP1
C-terminal cytoplasmic domain stably expressed in B cell lines. Our
results demonstrate that LMP1-stimulated Ig production, surface
molecule up-regulation, and NF-B and c-Jun N-terminal kinase
activation require both CTAR1 and CTAR2, and that these two regions may
interact to mediate LMP1 signaling. Furthermore, we find that the
function of CTAR1, but not CTAR2, correlates with TRAF binding and
present evidence that as yet unidentified cytoplasmic proteins may
associate with LMP1 to mediate some of its signaling
activities.
This article has been cited by other articles:
|
|
 |
|
  J. P. Graham, C. R. Moore, and G. A. Bishop Roles of the TRAF2/3 Binding Site in Differential B Cell Signaling by CD40 and Its Viral Oncogenic Mimic, LMP1 J. Immunol., September 1, 2009; 183(5): 2966 - 2973. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Ikeda, Y. Sekine, T. Yasui, K. Oritani, K. Sugiyma, R. Muromoto, N. Ohbayashi, A. Yoshimura, and T. Matsuda STAP-2 Negatively Regulates both Canonical and Noncanonical NF-{kappa}B Activation Induced by Epstein-Barr Virus-Derived Latent Membrane Protein 1 Mol. Cell. Biol., August 15, 2008; 28(16): 5027 - 5042. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Rowland, M. M. Tremblay, J. M. Ellison, L. L. Stunz, G. A. Bishop, and B. S. Hostager A Novel Mechanism for TNFR-Associated Factor 6-Dependent CD40 Signaling J. Immunol., October 1, 2007; 179(7): 4645 - 4653. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Mainou, D. N. Everly Jr., and N. Raab-Traub Unique Signaling Properties of CTAR1 in LMP1-Mediated Transformation J. Virol., September 15, 2007; 81(18): 9680 - 9692. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wu, P. Xie, K. Welsh, C. Li, C.-Z. Ni, X. Zhu, J. C. Reed, A. C. Satterthwait, G. A. Bishop, and K. R. Ely LMP1 Protein from the Epstein-Barr Virus Is a Structural CD40 Decoy in B Lymphocytes for Binding to TRAF3 J. Biol. Chem., September 30, 2005; 280(39): 33620 - 33626. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. T. Lu, R. L. Dryer, C. Song, and L. R. Covey Maintenance of the CD40-related immunodeficient response in hyper-IgM B cells immortalized with a LMP1-regulated mini-EBV J. Leukoc. Biol., September 1, 2005; 78(3): 620 - 629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Xie and G. A. Bishop Roles of TNF Receptor-Associated Factor 3 in Signaling to B Lymphocytes by Carboxyl-Terminal Activating Regions 1 and 2 of the EBV-Encoded Oncoprotein Latent Membrane Protein 1 J. Immunol., November 1, 2004; 173(9): 5546 - 5555. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Xie, B. S. Hostager, and G. A. Bishop Requirement for TRAF3 in Signaling by LMP1 But Not CD40 in B Lymphocytes J. Exp. Med., March 1, 2004; 199(5): 661 - 671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. S. Hostager, S. A. Haxhinasto, S. L. Rowland, and G. A. Bishop Tumor Necrosis Factor Receptor-associated Factor 2 (TRAF2)-deficient B Lymphocytes Reveal Novel Roles for TRAF2 in CD40 Signaling J. Biol. Chem., November 14, 2003; 278(46): 45382 - 45390. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ning, A. M. Hahn, L. E. Huye, and J. S. Pagano Interferon Regulatory Factor 7 Regulates Expression of Epstein-Barr Virus Latent Membrane Protein 1: a Regulatory Circuit J. Virol., September 1, 2003; 77(17): 9359 - 9368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Bishop, B. S. Hostager, and K. D. Brown Mechanisms of TNF receptor-associated factor (TRAF) regulation in B lymphocytes J. Leukoc. Biol., July 1, 2002; 72(1): 19 - 23. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. D. Brown, B. S. Hostager, and G. A. Bishop Regulation of TRAF2 Signaling by Self-induced Degradation J. Biol. Chem., May 24, 2002; 277(22): 19433 - 19438. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
