The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kollet, J.
Right arrow Articles by Petro, T. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kollet, J.
Right arrow Articles by Petro, T. M.
The Journal of Immunology, 2001, 167: 5653-5663.
Copyright © 2001 by The American Association of Immunologists

Deletional Analysis of the Murine IL-12 p35 Promoter Comparing IFN-{gamma} and Lipopolysaccharide Stimulation1

Jutta Kollet*, Christian Witek*, John D. Gentry*, Xiaojuan Liu*, Steven D. Schwartzbach{dagger} and Thomas M. Petro2,*

* Department of Oral Biology, University of Nebraska Medical Center, and {dagger} School of Biological Sciences, University of Nebraska, Lincoln, NE 68583

IL-12, pivotal to the development of Th1 cells and formed by association of p35 and p40 subunits, is made by macrophages and the macrophage cell line RAW264.7. In this study, the promoter for p35 was cloned and analyzed. The murine IL-12 p35 gene has promoters upstream from each of the first two exons. The exon 1 and exon 2 promoters, cloned into a reporter vector, were responsive to LPS or IFN-{gamma}/CD40 ligation in transfected RAW264.7 cells. The exon 2 promoter containing bp -809 to +1 has significant homology to the human p35 promoter. Thus, deletion analysis was performed to determine the regions required for responsiveness to LPS, CD40, and/or IFN-{gamma}. Base pairs -809 to -740 influenced responsiveness to LPS. In contrast, bp -740to -444 and bp -122 to -100 were required for responses to IFN-{gamma}, IFN-{gamma}/LPS, or IFN-{gamma}/CD40 ligation. Removal of bp -444 to -392 increased the response of the exon 2 promoter to each stimulant. IFN regulatory factor (IRF)-1 is involved in the activity of this promoter at bp -108 to -103 because levels of nuclear IRF-1 correlated with exon 2 promoter activity in response to IFN-{gamma} and IRF-1 overexpression stimulated and enhanced exon 2 promoter activity. Also, site or deletion mutation of the IRF-1 element at bp -108 to -103 reduced the responsiveness of the promoter and IRF-1 bound to an oligonucleotide containing bp -108 to -103. The data suggest that the response of the p35 promoter to IFN-{gamma} requires a distinct IRF-1 positive regulatory element at bp -108 to -103.




This article has been cited by other articles:


Home page
 Infect. Immun.Home page
A. Jayakumar, M. J. Donovan, V. Tripathi, M. Ramalho-Ortigao, and M. A. McDowell
Leishmania major Infection Activates NF-{kappa}B and Interferon Regulatory Factors 1 and 8 in Human Dendritic Cells
Infect. Immun., May 1, 2008; 76(5): 2138 - 2148.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. K. Polumuri, V. Y. Toshchakov, and S. N. Vogel
Role of Phosphatidylinositol-3 Kinase in Transcriptional Regulation of TLR-Induced IL-12 and IL-10 by Fc{gamma} Receptor Ligation in Murine Macrophages
J. Immunol., July 1, 2007; 179(1): 236 - 246.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
N. Nakano, C. Nishiyama, S. Kanada, Y. Niwa, N. Shimokawa, H. Ushio, M. Nishiyama, K. Okumura, and H. Ogawa
Involvement of mast cells in IL-12/23 p40 production is essential for survival from polymicrobial infections
Blood, June 1, 2007; 109(11): 4846 - 4855.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Liu, Z. Xiang, and X. Ma
Role of IFN Regulatory Factor-1 and IL-12 in Immunological Resistance to Pathogenesis of N-Methyl-N-Nitrosourea-Induced T Lymphoma
J. Immunol., July 15, 2004; 173(2): 1184 - 1193.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
J. Liu, S. Cao, L. M. Herman, and X. Ma
Differential Regulation of Interleukin (IL)-12 p35 and p40 Gene Expression and Interferon (IFN)-{gamma}-primed IL-12 Production by IFN Regulatory Factor 1
J. Exp. Med., October 20, 2003; 198(8): 1265 - 1276.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. Goriely, D. Demonte, S. Nizet, D. De Wit, F. Willems, M. Goldman, and C. Van Lint
Human IL-12(p35) gene activation involves selective remodeling of a single nucleosome within a region of the promoter containing critical Sp1-binding sites
Blood, June 15, 2003; 101(12): 4894 - 4902.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Cuesta, C. A. Salkowski, K. E. Thomas, and S. N. Vogel
Regulation of Lipopolysaccharide Sensitivity by IFN Regulatory Factor-2
J. Immunol., June 1, 2003; 170(11): 5739 - 5747.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Mirani, I. Elenkov, S. Volpi, N. Hiroi, G. P. Chrousos, and T. Kino
HIV-1 Protein Vpr Suppresses IL-12 Production from Human Monocytes by Enhancing Glucocorticoid Action: Potential Implications of Vpr Coactivator Activity for the Innate and Cellular Immunity Deficits Observed in HIV-1 Infection
J. Immunol., December 1, 2002; 169(11): 6361 - 6368.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2001 by The American Association of Immunologists, Inc. All rights reserved.